LOCUS       sequence-413616-       13042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of TAOK1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13042)
  AUTHORS   Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, 
            Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
  TITLE     Comprehensive split TEV based protein-protein interaction screening 
            reveals TAOK2 as a key modulator of Hippo signalling to limit 
            growth.
  JOURNAL   Cell Signal. 2024 Jan;113:110917. doi: 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
  PUBMED    37813295
REFERENCE   2  (bases 1 to 13042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Signal."; date: "2024-01"; volume: "113:110917. doi"; pages: " 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7"
COMMENT     Sequence Label: pINDUCER21-puro_TAOK1
FEATURES             Location/Qualifiers
     source          1..13042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2230..2248,2249..2271,2272..2290,2291..2313,2314..2332,
                     2333..2355,2356..2374,2375..2397,2398..2416,2417..2439,
                     2440..2458,2459..2481,2482..2500)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2533..2571
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2568..2592
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2701..2725
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5291..5315)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5368..5433
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             5451..5477
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5697..6875
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5928..6866
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6823..6843
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    6945..6978
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(6985..7602,7603..7608,7609..7644,7645..7647,7648..7683,
                     7684..7686,7687..7722,7723..7731)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    join(7784..8358,8359..8361,8362..8370)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7951..7968)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8178..8197
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8390..8989
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8390..8409)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8886..8906
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     9125..9144
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9432..10020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9485..9505)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9903..9914)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10095..10328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10447..10465)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10533..10637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10638..10706,10707..11498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10856..10875)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11669..12257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12158..12177
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12411..12428
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12668..12689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12704..12709,12710..12727,12728..12734)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12742..12758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12747..12769
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12766..12782
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12766..12782
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12832..12851
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagctcgg
     2521 tacccgggtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
     2581 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
     2641 gaccgatcca gcctccgcgg ccccgaacta gtccagtgtg gtggaattct gcagatatca
     2701 acaagtttgt acaaaaaagc aggctccacc atgccatcaa ctaacagagc aggcagcctg
     2761 aaggaccctg aaattgcaga gctcttcttc aaagaagatc cagagaagct cttcacagat
     2821 ctcagagaaa ttggccatgg aagctttgga gcagtgtatt ttgcacgaga tgtgcgtacc
     2881 aatgaagtgg tggccatcaa gaaaatgtct tatagtggaa agcagtctac tgagaaatgg
     2941 caggatatta ttaaggaagt caagtttcta caaagaataa aacatcccaa cagtatagaa
     3001 tacaaaggct gttatttacg tgaacacaca gcatggcttg taatggaata ttgtttagga
     3061 tctgcttcgg atttactaga agttcacaaa aagccattac aagaagtgga aatagcagca
     3121 attacacatg gtgctcttca gggattagcc tacttacatt ctcatactat gattcataga
     3181 gatatcaaag caggaaatat ccttctgaca gaaccaggcc aggtgaaact tgctgacttt
     3241 ggctctgctt ccatggcatc acctgccaat tcctttgtgg gaacgccgta ttggatggcc
     3301 ccagaagtaa ttttagccat ggatgaagga caatatgatg gcaaagtaga tgtgtggtct
     3361 cttggaataa catgtattga actagcggaa aggaagcctc ctttatttaa tatgaatgca
     3421 atgagtgcct tatatcacat agcccaaaat gaatccccta cactacagtc taatgaatgg
     3481 tctgattatt ttcgcaactt tgtagattct tgcctccaga aaatccctca agatcgacct
     3541 acatcagagg aacttttaaa gcacatattt gttcttcggg agcgccctga aaccgtgtta
     3601 atagatctca ttcagaggac aaaggatgca gtaagagagc tggacaatct gcagtatcga
     3661 aagatgaaga aactcctttt ccaggaggca cataatggac cagcagtaga agcacaggaa
     3721 gaagaagagg aacaagatca tggtgttggc cggacaggaa cagttaatag tgttggaagt
     3781 aatcaatcca ttcccagcat gtccatcagt gccagcagcc aaagcagtag tgttaacagt
     3841 cttccagatg tctcagatga caagagtgag ctagacatga tggagggaga ccacacagtg
     3901 atgtctaaca gttctgttat ccatttaaaa ccagaggaag aaaattacag agaagaggga
     3961 gatcctagaa caagagcatc agatccacaa tctccacccc aagtatctcg tcacaaatca
     4021 cactatcgta atcgagaaca ctttgctact atacggacag catcactggt tacgaggcaa
     4081 atgcaagaac atgagcagga ctctgagctt agagaacaaa tgtctggcta taagcgaatg
     4141 aggcgacaac atcaaaagca actgatgact ctggaaaaca agctaaaggc tgagatggat
     4201 gaacatcgcc tcagattaga caaagatctt gaaactcagc gtaacaattt tgctgcagaa
     4261 atggagaaac ttatcaagaa acaccaggct gctatggaga aagaggctaa agtgatgtcc
     4321 aatgaagaga aaaaatttca gcaacatatt caggcccaac agaagaaaga actgaatagt
     4381 tttctcgagt cccagaaaag agagtataaa cttcgaaaag agcagcttaa agagtctaaa
     4441 gaactccaaa taaaaaagca gtttcaggat acctgcaaaa tccaaaccag acagtacaaa
     4501 gcattaagaa atcacctgct ggagactaca ccaaagagtg agcacaaagc tgttctgaaa
     4561 cggctcaagg aggaacagac ccggaaatta gctatcttgg ctgagcagta tgatcacagc
     4621 attaatgaaa tgctctccac acaagccctg cgtttggatg aagcacagga agcagagtgc
     4681 caggttttga agatgcagct gcagcaggaa ctggagctgt tgaatgcgta tcagagcaaa
     4741 atcaagatgc aagctgaggc acaacatgat cgagagcttc gcgagcttga acagagggtc
     4801 tccctccgga gggcactctt agaacaaaag attgaagaag agatgttggc tttgcagaat
     4861 gagcgcacag aacgaatacg aagcctgttg gaacgtcaag ccagagagat tgaagctttt
     4921 gactctgaaa gcatgagact aggttttagt aatatggtcc tttctaatct ctcccctgag
     4981 gcattcagcc acagctaccc gggagcttct ggttggtcac acaaccctac tgggggtcca
     5041 ggacctcact ggggtcatcc catgggtggc ccaccacaag cttggggcca tccaatgcaa
     5101 ggtggacccc agccatgggg tcacccttca gggccaatgc aaggggtacc tcgaggtagc
     5161 agtatgggag tccgcaatag cccccaggct ctgaggcgga cagcttctgg gggacggaca
     5221 gagcagggca tgagcagaag cacgagtgtc acttcacaaa tatccaatgg gtcacacatg
     5281 tcttatacag acccagcttt cttgtacaaa gtggttgata tccagcacag tggcggccgc
     5341 tcgagtctag agggcccgcg gttcgaagac tacaaagacc atgacggtga ttataaagat
     5401 catgacatcg attacaagga tgacgatgac aagtaggtaa gtgattcgaa tacccatacg
     5461 acgtcccaga ctacgcttag taatgattaa ttaaactaga aattctaccg ggtaggggag
     5521 gcgcttttcc caaggcagtc tggagtaacc cacccaagat ctggcctccg cgccgggttt
     5581 tggcgcctcc cgcgggcgcc cccctcctca cggcgagccg cgtaagatct ggcctccgcg
     5641 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcctag tcgtgaggct
     5701 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     5761 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     5821 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     5881 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     5941 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     6001 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     6061 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     6121 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     6181 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     6241 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     6301 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     6361 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     6421 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     6481 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     6541 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     6601 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     6661 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     6721 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     6781 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     6841 ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgccatgct agtaaattgt
     6901 ccgctaaatt ctggccgttt ttggcttttt tgttagacgg atccataact tcgtataatg
     6961 tatgctatac gaagttatgc caccatgtct agactggaca agagcaaagt cataaacgga
     7021 gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     7081 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     7141 gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc
     7201 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     7261 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     7321 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     7381 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     7441 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa
     7501 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     7561 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     7621 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     7681 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta actaagtaag
     7741 gatccgcggc cgcactagag gaattccgcc cctctccccc ccccccctct ccctcccccc
     7801 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     7861 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     7921 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     7981 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     8041 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     8101 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     8161 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     8221 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg
     8281 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     8341 gaaaaacacg atgataagct tgccacaacc cacaaggaga cgaccttcca tgaccgagta
     8401 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc
     8461 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga
     8521 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     8581 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     8641 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     8701 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     8761 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     8821 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     8881 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     8941 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgag catgcccgac
     9001 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     9061 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     9121 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     9181 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     9241 gacgagttct tctgatgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
     9301 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     9361 acataaaatg aatgcaattg ttgttgttta gtccctccca attcgatatc aagcttatcg
     9421 cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     9481 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     9541 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     9601 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     9661 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     9721 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     9781 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     9841 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     9901 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     9961 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    10021 ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
    10081 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
    10141 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
    10201 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
    10261 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
    10321 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
    10381 aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat tcttgaagac
    10441 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
    10501 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    10561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    10621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    10681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    10741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    10801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    10861 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
    10921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    10981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    11041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    11101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    11161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    11221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    11281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    11341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    11401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    11461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    11521 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    11581 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    11641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    11701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    11761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    11821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    11881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    11941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    12001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    12061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    12121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    12181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    12241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    12301 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    12361 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    12421 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    12481 ttcattaatg cagcaagctc atggctgact aatttttttt atttatgcag aggccgaggc
    12541 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    12601 ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
    12661 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
    12721 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
    12781 acatgattac gaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    12841 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    12901 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    12961 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    13021 tatgtactac aaacttagta gt
//
