LOCUS       sequence-413617-       10569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRi for STK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Ma X, Mandausch FJ, Wu Y, Sahoo VK, Ma W, Leoni G, Hostiuc M, 
            Wintgens JP, Qiu J, Kannaiyan N, Rossner MJ, Wehr MC
  TITLE     Comprehensive split TEV based protein-protein interaction screening 
            reveals TAOK2 as a key modulator of Hippo signalling to limit 
            growth.
  JOURNAL   Cell Signal. 2024 Jan;113:110917. doi: 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7.
  PUBMED    37813295
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Signal."; date: "2024-01"; volume: "113:110917. doi"; pages: " 
            10.1016/j.cellsig.2023.110917. Epub 2023 Oct 7"
COMMENT     Sequence Label: pLenti7-3_EF1ap-mRuby2_hU6p_sgSTK4i_#1 (BlasR)
FEATURES             Location/Qualifiers
     source          1..10569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1955..1971
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1977..2201
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    2201..2790
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2308..2319
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2717..2737
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2802..3197)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(3198..3254)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(3264..3974)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     regulatory      3971..3980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3988..5166)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3997..4935
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(4020..4040)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5189..5437
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5189..5209
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5360..5379
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     polyA_signal    5576..5624
                     /note="synthetic polyadenylation signal"
     promoter        5658..5676
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(5726..5744)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(5788..6303)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     regulatory      6300..6309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6402..6423)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6689..6708
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6948..6969
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     LTR             7090..7323
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7395..7516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7432..7451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7486..7505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7556..7691
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(7712..7730)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7740..7756)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7749..7771)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7898..8353
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7985..8004)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8195..8216
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8379..8483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8484..8552,8553..9344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8702..8721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9515..10103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10004..10023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10257..10274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10391..10412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10427..10432,10433..10450,10451..10457)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10465..10481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10470..10492
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10489..10505
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10489..10505
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        10526..10544
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgaaccc ttcgatcaac tcgcgccgct ggtcgtaaaa cgacggccag ttcgaaccat
     1981 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     2041 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     2101 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     2161 ggaggggcaa acaacagatg gctggcaact agaaggcaca gcgggggagg cggcccaaag
     2221 ggagatccga ctcgtctgag ggcgaaggcg tagacgcgga agaggccgca gagccggcag
     2281 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
     2341 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
     2401 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
     2461 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
     2521 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
     2581 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
     2641 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
     2701 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
     2761 ttcacaaatt ttgtaatcca gaggttgatt atcgaacgcg tttagccctc ccacacataa
     2821 ccagagggca gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact
     2881 atggctttga tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc
     2941 aagatgcccc tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca
     3001 gcagcagcag tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca
     3061 ttgacaccag tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct
     3121 tcagagatgg ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct
     3181 tgagacaaag gcttggccgg tccaggattc tcttcgacat ctccggcttg tttcagcaga
     3241 gagaagtttg ttgcgcctga tcccttgtac agctcgtcca tcccaccacc aagcccggcg
     3301 aacttggcaa ctgcgtgttc gcgttgtact acgaacattt cattgtcact ttcctctaac
     3361 ctttccaggc ggtgatcaac ggcatggata ccgggcatct tgatgttccc gacggtcttt
     3421 tttgacctgt aagttgttac gaaagagcaa gacagatggc caccaccatc aactttcagt
     3481 gccatatgag tgtatcccct cagaccacca tctgctggat acatcatctc tgtattaggc
     3541 tcccaaccct tggtcttctt ctgcatcacg ggaccattgg agggaaagtt tacccctctg
     3601 acttggacgt ggtaaacgag acagccatcc tcaaggctgg tgtcctgcat gacggtgacg
     3661 actccaccat cttcgtatct cgtaactctt tcccaagtaa aaccctcagg aaaggactgt
     3721 ttaaagaaat caggaatgcc tttcgggtac ttgataaaag tacggctgcc atacatgaac
     3781 gacgtggcaa gaatgtcaaa ggcaaatggc aggggtcctc cctcgatgac tttgatcctc
     3841 atggtttgag ttcccatgta cggattgcct tctccttcac ctgtgcattt gaattggtgg
     3901 ccgttgaccg aaccttccat gaccaccttc atacgcatat tttccttgat cagctcttcg
     3961 cccttagaca ccatggtggc gtcgaattca cgacacctga aatggaagaa aaaaactttg
     4021 aaccactgtc tgaggcttga gaatgaacca agatccaaac tcaaaaaggg caaattccaa
     4081 ggagaattac atcaagtgcc aagctggcct aacttcagtc tccacccact cagtgtgggg
     4141 aaactccatc gcataaaacc cctcccccca acctaaagac gacgtactcc aaaagctcga
     4201 gaactaatcg aggtgcctgg acggcgcccg gtactccgtg gagtcacatg aagcgacggc
     4261 tgaggacgga aaggcccttt tcctttgtgt gggtgactca cccgcccgct ctcccgagcg
     4321 ccgcgtcctc cattttgagc tccctgcagc agggccggga agcggccatc tttccgctca
     4381 cgcaactggt gccgaccggg ccagccttgc cgcccagggc ggggcgatac acggcggcgc
     4441 gaggccaggc accagagcag gccggccagc ttgagactac ccccgtccga ttctcggtgg
     4501 ccgcgctcgc aggccccgcc tcgccgaaca tgtgcgctgg gacgcacggg ccccgtcgcc
     4561 gcccgcggcc ccaaaaaccg aaataccagt gtgcagatct tggcccgcat ttacaagact
     4621 atcttgccag aaaaaaagcg tcgcagcagg tcatcaaaaa ttttaaatgg ctagagactt
     4681 atcgaaagca gcgagacagg cgcgaaggtg ccaccagatt cgcacgcggc ggccccagcg
     4741 cccaggccag gcctcaactc aagcacgagg cgaaggggct ccttaagcgc aaggcctcga
     4801 actctcccac ccacttccaa cccgaagctc gggatcaaga atcacgtact gcagccaggt
     4861 ggaagtaatt caaggcacgc aagggccata acccgtaaag aggccaggcc cgcgggaacc
     4921 acacacggca cttacctgtg ttctggcggc aaacccgttg cgaaaaagaa cgttcacggc
     4981 gactactgca cttatatacg gttctccccc accctcggga aaaaggcgga gccagtacac
     5041 gacatcactt tcccagttta ccccgcgcca ccttctctag gcaccggttc aattgccgac
     5101 ccctcccccc aacttctcgg ggactgtggg cgatgtgcgc tctgcccact gacgggcacc
     5161 ggagccatcg atggccgccc ccttcaccga gggcctattt cccatgattc cttcatattt
     5221 gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa
     5281 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt
     5341 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt
     5401 cttggcttta tatatcttgt ggaaaggacg aaacaccggg gaggatggag cagtgagcgt
     5461 ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt
     5521 gaaaaagtgg caccgagtcg gtgcctgaat gcctgcgagc atctttttta ctagtcacac
     5581 aaaaaaccaa cacacagatc taatgaaaat aaagatcttt tattgtctag atcgtacatg
     5641 cattgactcg cgtctactaa tacgactcac tataggactg atgcatcgac gtagtctgac
     5701 agtcgtacta gatagtgtca cctacccttt agtgagggtt aattctactc ggcagactta
     5761 gcaactagct ggatccgata tcttaagtta cgccagaatg cgttcgcaca gccgccagcc
     5821 ggtcactccg ttgatggtta ctcggaacag cagggagccg tcggggttga tcaggcgctc
     5881 gtcgataatt ttgttgccgt tccacagggt ccctgttaca gtgatctttt tgccgtcgaa
     5941 cacggcgatg ccttcatacg gccgtccgaa atagtcgatc atgttcggcg taaccccgtc
     6001 gattaccagt gtgccatagt gcaggatcac cttaaagtga tgatcatcca cagggtacac
     6061 caccttaaaa attttttcga tctggcccat ttggtcgccg ctcagacctt catacgggat
     6121 gatgacatgg atgtcgatct tcagcccatt ttcaccgctc aggacaatcc tttggatcgg
     6181 agttacggac accccgagat tctgaaacaa actggacaca cctccctgtt caaggacttg
     6241 gtccaggttg tagccggctg tctgtcgcca gtccccaacg aaatcttcga gtgtgaacac
     6301 catggtggcg tcaagcttgg ccgccgaggc cagatcaatt gatatcctcg aggctagcgc
     6361 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
     6421 atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct caggtagtgg
     6481 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
     6541 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga atgtactcca gcttgtgccc
     6601 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     6661 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     6721 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     6781 gtggtcgggg tagcgggcga agcactgcac gccccaggtc agggtggtca cgagggtggg
     6841 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     6901 ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag
     6961 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg ccccctgcag gcagctcagg
     7021 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7081 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     7141 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7201 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7261 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7321 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7381 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7441 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7501 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7561 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     7621 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     7681 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     7741 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7801 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7861 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     7921 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7981 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8041 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8101 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8161 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8221 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8281 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8341 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     8401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     8461 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     8521 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     8581 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     8641 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     8701 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     8761 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     8821 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     8881 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     8941 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     9001 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     9061 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     9121 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     9181 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     9241 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     9301 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     9361 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     9421 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     9481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     9541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     9601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     9661 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     9721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     9781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     9841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     9901 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     9961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    10021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    10081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    10141 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    10201 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    10261 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    10321 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    10381 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    10441 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    10501 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    10561 tgcaagctt
//
