LOCUS       sequence-413650-        5688 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter under the control of the Dlx promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Fisher J, Verhagen M, Long Z, Moissidis M, Yan Y, He C, Wang J, 
            Micoli E, Alastruey CM, Moors R, Marin O, Mi D, Lim L
  TITLE     Cortical somatostatin long-range projection neurons and interneurons
            exhibit divergent developmental trajectories.
  JOURNAL   Neuron. 2023 Dec 7:S0896-6273(23)00887-5. doi: 
            10.1016/j.neuron.2023.11.013.
  PUBMED    38086373
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2023 Dec 7:S0896-6273(23)00887-5. doi: 
            10.1016/j.neuron.2023.11.013."
COMMENT     Sequence Label: p164-pAAV-mDlx-FLEX-mScarlet
FEATURES             Location/Qualifiers
     source          1..5688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      678..1266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1167..1186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   1328..1457
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     intron          2176..2308
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2291..2310
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     protein_bind    complement(2365..2398)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(2409..2442)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(2462..3157)
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2737..2756)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3006..3024
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    3234..3267
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    3278..3311
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     misc_feature    3361..3949
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3414..3434)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3832..3843)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4118..4199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4155..4174)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4209..4228
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   4278..4418
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4278..4407
                     /label=AAV2 ITR
     rep_origin      4493..4948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4580..4599)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4790..4811
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4965..4984)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5084..5106
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5144..5162)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5230..5334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5335..5403,5404..5688,1..507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5553..5572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
       61 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      121 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      181 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      241 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      301 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      361 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      421 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      481 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
      541 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
      601 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      661 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      721 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
      781 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
      841 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
      901 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
      961 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1021 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1081 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1141 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1201 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1261 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1321 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
     1381 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
     1441 catcactagg ggttcctgcg gccgcacgcg tttaattaag aattctatac actcacagtg
     1501 gtttggcata tatttggtga aattttttaa ggaaaaatta gtgttggttt cgatatatgg
     1561 tagctttttc tctaacataa tttgaataat tcagcaaagc cctactacca gctgtacttc
     1621 tgcagcctct tccattcttt ccagcattat aattttggtt aattttcaat tttaggtcct
     1681 acgtctctgc aatttgtgta tgaataacag aataatttcc ctcttttgtt tcgcctttcc
     1741 tgttcctgaa tctaaataaa gatggctttt tagtattaaa agtggaagaa aattacaggt
     1801 aattatcttt gacggtaaaa acgctgtaat cagcgggcta catgaaaaat tactctaatt
     1861 atggctgcat ttaagagaat ggaaaaaaac cttcttgtgg ataaaaacct taaattgtcc
     1921 ccaatgtctg cttcaaattg gatggcactg cagctggagg ctttgttcag aattgatcct
     1981 ggggagctac gaacccaaag tttcacagta ggaagcttga tatcgaattc ctgcagcccg
     2041 ggctgggcat aaaagtcagg gcagagccat ctattgctta catttgcttc tagcctgcag
     2101 gtcgaggagc gcagccttcc agaagcagag cgcggcgcct taagctgcag aagttggtcg
     2161 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa
     2221 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact
     2281 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa
     2341 gaaactagta gccactcgac gtcgataact tcgtatagca tacattatac gaagttatcc
     2401 gaatcacaat aacttcgtat aggatacttt atacgaagtt atttggcgcg ccgctagctt
     2461 acttgtacag ctcgtccatg ccgccggtgg agtggcggcc ctcggagcgt tcgtactgtt
     2521 ccaccacggt gtagtcctcg ttgtgggagg tgatgtccaa cttgcggtcg acgttgtagg
     2581 cgccgggcat ctgcacgggc ttcttggcct tgtaggtggt cttgaagtcc gccaggtagc
     2641 ggccgccgtc cttcaggcgc agggccatct taatgtcgcc cttcagcacg ccgtcctcgg
     2701 ggtacaaccg ctcggtggac gcttcccagc ccattgtctt cttctgcatt acggggccgt
     2761 caggagggaa gttggtgccg cggagcttca ccttgtagat cagggtgccg tcctccaggg
     2821 aggtgtcctg ggtcacggtc acggcgccgc cgtcctcgaa gttcatcacg cgctcccact
     2881 tgaagccctc ggggaaggac tgcttatagt agtcggggat gtcggcgggg tgcttgatga
     2941 aggccctgga gccgtacatg aactgagggg acaggatgtc ccaggagaag ggcagggggc
     3001 cacccttggt caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct
     3061 cgccctcgat ctcgaactcg tggccgttca tggagccctc catgtgcacc ttgaaccgca
     3121 tgaactcctt gatcactgcc tcgcccttgc tcaccatgat cttttggtcc tcatcattct
     3181 tttcaacctg tttggttctt ctcatacagc acagcatggt ggggccggcc ttgataactt
     3241 cgtataatgt atgctatacg aagttatccg aatcgcaata acttcgtata aagtatccta
     3301 tacgaagtta tcgaagatca tcaccacccc tgcagggaat tcgatatcaa gcttatcgat
     3361 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3421 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3481 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3541 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3601 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3661 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3721 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     3781 gcctatgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     3841 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     3901 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccga
     3961 gcgctgctcg agagatctgt gatagcggcc atcaagctgg ccgcgactct agatcataat
     4021 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     4081 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     4141 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4201 ttctagttgt ggtttgtcca aactcatcaa tgtatcagct tatcgatacc gcatgcacgt
     4261 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     4321 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     4381 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     4441 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     4501 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4561 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4621 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4681 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     4741 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4801 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     4861 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4921 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     4981 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     5041 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     5101 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     5161 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     5221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaaga
//
