LOCUS       sequence-413652-       12554 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing GLI1-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12554)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 12554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-GLI1-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..12554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..816
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             834..1014
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1061..1186
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1679..1912
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2097..2141
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2439..2556
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2613..2631,2632..2654,2655..2673,2674..2696,2697..2715,
                     2716..2738,2739..2757,2758..2780,2781..2799,2800..2822,
                     2823..2841,2842..2864,2865..2883)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2916..2954
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2951..2975
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6403..6459
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6469..7176
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6611..6629)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6879..6898
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7199..7787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7252..7272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7670..7681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7790..7806)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7791..7807)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8358..8538
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8564..8581)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8570..8794
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8840..9268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8927..8946)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9137..9158
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9277..9297)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9282..9611
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9462..9597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9524..9543
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9659..9706
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9725..10099
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10229..10350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10266..10285)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10320..10339
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10399..10415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10399..10415)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10412..10434)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10423..10439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10447..10453,10454..10471,10472..10477))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10492..10513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10630..10647)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10801..11389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10881..10900)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11560..12351,12352..12420))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12183..12202
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12421..12525)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      841 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      901 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      961 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     1021 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     1081 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1141 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1201 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1261 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1321 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1381 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1441 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1501 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1561 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1621 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1681 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1741 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1801 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1861 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1921 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1981 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     2041 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     2101 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2161 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2221 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2281 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2341 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2401 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2461 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2521 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2581 gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     2761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     2821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2881 agaaaagtgg aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct
     2941 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     3001 ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa ttccgccacc
     3061 atgttcaact cgatgacccc accaccaatc agtagctatg gcgagccctg ctgtctccgg
     3121 cccctcccca gtcagggggc ccccagtgtg gggacagaag gactgtctgg cccgcccttc
     3181 tgccaccaag ctaacctcat gtccggcccc cacagttatg ggccagccag agagaccaac
     3241 agctgcaccg agggcccact cttttcttct ccccggagtg cagtcaagtt gaccaagaag
     3301 cgggcactgt ccatctcacc tctgtcggat gccagcctgg acctgcagac ggttatccgc
     3361 acctcaccca gctccctcgt agctttcatc aactcgcgat gcacatctcc aggaggctcc
     3421 tacggtcatc tctccattgg caccatgagc ccatctctgg gattcccagc ccagatgaat
     3481 caccaaaaag ggccctcgcc ttcctttggg gtccagcctt gtggtcccca tgactctgcc
     3541 cggggtggga tgatcccaca tcctcagtcc cggggaccct tcccaacttg ccagctgaag
     3601 tctgagctgg acatgctggt tggcaagtgc cgggaggaac ccttggaagg tgatatgtcc
     3661 agccccaact ccacaggcat acaggatccc ctgttgggga tgctggatgg gcgggaggac
     3721 ctcgagagag aggagaagcg tgagcctgaa tctgtgtatg aaactgactg ccgttgggat
     3781 ggctgcagcc aggaatttga ctcccaagag cagctggtgc accacatcaa cagcgagcac
     3841 atccacgggg agcggaagga gttcgtgtgc cactgggggg gctgctccag ggagctgagg
     3901 cccttcaaag cccagtacat gctggtggtt cacatgcgca gacacactgg cgagaagcca
     3961 cacaagtgca cgtttgaagg gtgccggaag tcatactcac gcctcgaaaa cctgaagacg
     4021 cacctgcggt cacacacggg tgagaagcca tacatgtgtg agcacgaggg ctgcagtaaa
     4081 gccttcagca atgccagtga ccgagccaag caccagaatc ggacccattc caatgagaag
     4141 ccgtatgtat gtaagctccc tggctgcacc aaacgctata cagatcctag ctcgctgcga
     4201 aaacatgtca agacagtgca tggtcctgac gcccatgtga ccaaacggca ccgtggggat
     4261 ggccccctgc ctcgggcacc atccatttct acagtggagc ccaagaggga gcgggaagga
     4321 ggtcccatca gggaggaaag cagactgact gtgccagagg gtgccatgaa gccacagcca
     4381 agccctgggg cccagtcatc ctgcagcagt gaccactccc cggcagggag tgcagccaat
     4441 acagacagtg gtgtggaaat gactggcaat gcagggggca gcactgaaga cctctccagc
     4501 ttggacgagg gaccttgcat tgctggcact ggtctgtcca ctcttcgccg ccttgagaac
     4561 ctcaggctgg accagctaca tcaactccgg ccaataggga cccggggtct caaactgccc
     4621 agcttgtccc acaccggtac cactgtgtcc cgccgcgtgg gccccccagt ctctcttgaa
     4681 cgccgcagca gcagctccag cagcatcagc tctgcctata ctgtcagccg ccgctcctcc
     4741 ctggcctctc ctttcccccc tggctcccca ccagagaatg gagcatcctc cctgcctggc
     4801 cttatgcctg cccagcacta cctgcttcgg gcaagatatg cttcagccag agggggtggt
     4861 acttcgccca ctgcagcatc cagcctggat cggataggtg gtcttcccat gcctccttgg
     4921 agaagccgag ccgagtatcc aggatacaac cccaatgcag gggtcacccg gagggccagt
     4981 gacccagccc aggctgctga ccgtcctgct ccagctagag tccagaggtt caagagcctg
     5041 ggctgtgtcc ataccccacc cactgtggca gggggaggac agaactttga tccttacctc
     5101 ccaacctctg tctactcacc acagcccccc agcatcactg agaatgctgc catggatgct
     5161 agagggctac aggaagagcc agaagttggg acctccatgg tgggcagtgg tctgaacccc
     5221 tatatggact tcccacctac tgatactctg ggatatgggg gacctgaagg ggcagcagct
     5281 gagccttatg gagcgagggg tccaggctct ctgcctcttg ggcctggtcc acccaccaac
     5341 tatggcccca acccctgtcc ccagcaggcc tcatatcctg accccaccca agaaacatgg
     5401 ggtgagttcc cttcccactc tgggctgtac ccaggcccca aggctctagg tggaacctac
     5461 agccagtgtc ctcgacttga acattatgga caagtgcaag tcaagccaga acaggggtgc
     5521 ccagtggggt ctgactccac aggactggca ccctgcctca atgcccaccc cagtgagggg
     5581 cccccacatc cacagcctct cttttcccat tacccccagc cctctcctcc ccaatatctc
     5641 cagtcaggcc cctataccca gccaccccct gattatcttc cttcagaacc caggccttgc
     5701 ctggactttg attcccccac ccattccaca gggcagctca aggctcagct tgtgtgtaat
     5761 tatgttcaat ctcaacagga gctactgtgg gagggtgggg gcagggaaga tgcccccgcc
     5821 caggaacctt cctaccagag tcccaagttt ctggggggtt cccaggttag cccaagccgt
     5881 gctaaagctc cagtgaacac atatggacct ggctttggac ccaacttgcc caatcacaag
     5941 tcaggttcct atcccacccc ttcaccatgc catgaaaatt ttgtagtggg ggcaaatagg
     6001 gcttcacata gggcagcagc accacctcga cttctgcccc cattgcccac ttgctatggg
     6061 cctctcaaag tgggaggcac aaaccccagc tgtggtcatc ctgaggtggg caggctagga
     6121 gggggtcctg ccttgtaccc tcctcccgaa ggacaggtat gtaaccccct ggactctctt
     6181 gatcttgaca acactcagct ggactttgtg gctattctgg atgagcccca ggggctgagt
     6241 cctcctcctt cccatgatca gcggggcagc tctggacata ccccacctcc ctctgggccc
     6301 cccaacatgg ctgtgggcaa catgagtgtc ttactgagat ccctacctgg ggaaacagaa
     6361 ttcctcaact ctagtgccac gcgtgaattc gatggatccg gggccaccaa cttttccctg
     6421 ctgaagcagg ctggcgacgt cgaggagaac cctggccctg gctccggagt gagcaagggc
     6481 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     6541 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     6601 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     6661 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     6721 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     6781 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     6841 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     6901 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     6961 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     7021 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     7081 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     7141 cactccaccg gcggcatgga cgagctgtac aagtgaatct agaacgcgtt aagtcgacaa
     7201 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7261 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7321 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7381 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7441 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7501 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7561 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     7621 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7681 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7741 ccttcgccct cagacgagtc ggatctccct ttgggccgct tccccgcatc gataccgtcg
     7801 acctcgagac ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     7861 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     7921 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     7981 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg
     8041 gatctaccac acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat
     8101 cagatatcca ctgacctttg gatggtgcta caaggctagg tgaagagtca cggacaacta
     8161 ggtatcctga gctagcagaa ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     8221 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     8281 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     8341 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     8401 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     8461 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     8521 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     8581 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     8641 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     8701 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     8761 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     8821 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     8881 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     8941 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     9001 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     9061 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9121 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9181 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     9241 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     9301 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     9361 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     9421 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     9481 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     9541 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     9601 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     9661 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     9721 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     9781 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     9841 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     9901 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     9961 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    10021 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    10081 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    10141 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    10201 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    10261 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    10321 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    10381 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10441 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10501 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11221 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12301 tgcacccaac tgatcttcag catcttttac ttttaccagc gtttctgggt gagcaaaaac
    12361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12541 agtgccacct gacg
//
