LOCUS       sequence-413659-       13245 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing TRPS1-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13245)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 13245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-TRPS1-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..13245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..85)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        257..636
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        638..836
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             854..1034
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1081..1206
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1699..1932
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2117..2161
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2459..2576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2633..2651,2652..2674,2675..2693,2694..2716,2717..2735,
                     2736..2758,2759..2777,2778..2800,2801..2819,2820..2842,
                     2843..2861,2862..2884,2885..2903)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2936..2974
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2971..2995
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6987..7043
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7053..7760
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(7195..7213)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7463..7482
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7783..8371
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7836..7856)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8254..8265)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8374..8390)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8375..8391)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9069..9249
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9275..9292)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9281..9505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9551..9979
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9638..9657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9848..9869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9988..10008)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9993..10322
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10173..10308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10235..10254
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10370..10417
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10436..10810
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10940..11061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10977..10996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11031..11050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11110..11126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11110..11126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11123..11145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11134..11150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11158..11164,11165..11182,11183..11188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11203..11224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11341..11358)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11512..12100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11592..11611)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12271..13062,13063..13131))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12894..12913
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13132..13236)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
       61 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
      121 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
      181 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
      241 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
      301 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      361 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      421 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      481 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      541 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      601 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      661 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      721 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      781 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
      841 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      901 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      961 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     1021 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     1081 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     1141 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     1201 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     1261 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     1321 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     1381 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     1441 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     1501 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     1561 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     1621 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     1681 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     1741 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     1801 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     1861 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     1921 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     1981 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     2041 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     2101 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     2161 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     2221 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     2281 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     2341 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     2401 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     2461 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     2521 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     2581 tttattacag ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc
     2641 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2701 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2761 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     2821 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2881 ccactcccta tcagtgatag agaaaagtgg aagtcgagct cggtacccgg gtcgaggtag
     2941 gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     3001 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     3061 cggccccgaa ttccgccacc atgccttatg aagtcaatgc tgggtatgat tttacaaata
     3121 tggtccggaa aaagaacccc cctctgagaa acgttgcaag tgaaggcgag ggccagatcc
     3181 tggagcctat aggtacagaa agcaaggtat ctggaaagaa caaagaattt tctgcagatc
     3241 agatgtcaga aaatacggat cagagtgatg ctgcagaact aaatcataag gaggaacata
     3301 gcttgcatgt tcaagatcca tcttctagca gtaagaagga cttgaaaagc gcagttctga
     3361 gtgagaaggc tggcttcaat tatgaaagcc ccagtaaggg aggaaacttt ccctcctttc
     3421 cgcatgatga ggtgacagac agaaatatgt tggctttctc atctccagct gctgggggag
     3481 tctgtgagcc cttgaagtct ccgcaaagag cagaggcaga tgaccctcaa gatatggcct
     3541 gcaccccctc aggggactca ctggagacaa aggaagatca gaagatgtca ccaaaggcta
     3601 cagaggaaac agggcaagca cagagtggtc aagccaattg tcaaggtttg agcccagttt
     3661 cagtggcctc aaaaaaccca caagtgcctt cagatggggg tgtaagactg aataaatcca
     3721 aaactgactt actggtgaat gacaacccag acccggcacc tctgtctcca gagcttcagg
     3781 actttaaatg caatatctgt ggatatggtt actacggcaa cgaccccaca gatctgatta
     3841 agcacttccg aaagtatcac ttaggactgc ataaccgcac caggcaagat gctgagctgg
     3901 acagcaaaat cttggccctt cataacatgg tgcagttcag ccattccaaa gacttccaga
     3961 aggtcaaccg ttctgtgttt tctggtgtgc tgcaggacat caattcttca aggcctgttt
     4021 tactaaatgg gacctatgat gtgcaggtga cttcaggtgg aacattcatt ggcattggac
     4081 ggaaaacacc agattgccaa gggaacacca agtatttccg ctgtaaattc tgcaatttca
     4141 cttatatggg caactcatcc accgaattag aacaacattt tcttcagact cacccaaaca
     4201 aaataaaagc ttctctcccc tcctctgagg ttgcaaaacc ttcagagaaa aactctaaca
     4261 agtccatccc tgcacttcaa tccagtgatt ctggagactt gggaaaatgg caggacaaga
     4321 taacagtcaa agcaggagat gacactcctg ttgggtactc agtgcccata aagcccctcg
     4381 attcctctag acaaaatggt acagaggcca ccagttacta ctggtgtaaa ttttgtagtt
     4441 tcagctgtga gtcatctagc tcacttaaac tgctagaaca ttatggcaag cagcacggag
     4501 cagtgcagtc aggcggcctt aatccagagt taaatgataa gctttccagg ggctctgtca
     4561 ttaatcagaa tgatctagcc aaaagttcag aaggagagac aatgaccaag acagacaaga
     4621 gctcgagtgg ggctaaaaag aaggacttct ccagcaaggg agccgaggat aatatggtaa
     4681 cgagctataa ttgtcagttc tgtgacttcc gatattccaa aagccatggc cctgatgtaa
     4741 ttgtagtggg gccacttctc cgtcattatc aacagctcca taacattcac aagtgtacca
     4801 ttaaacactg tccattctgt cccagaggac tttgcagccc agaaaagcac cttggagaaa
     4861 ttacttatcc gtttgcttgt agaaaaagta attgttccca ctgtgcactc ttgcttctgc
     4921 acttgtctcc tggggcggct ggaagctcgc gagtcaaaca tcagtgccat cagtgttcat
     4981 tcaccacccc tgacgtagat gtactcctct ttcactatga aagtgtgcat gagtcccaag
     5041 catcggatgt caaacaagaa gcaaatcacc tgcaaggatc ggatgggcag cagtctgtca
     5101 aggaaagcaa agaacactca tgtaccaaat gtgattttat tacccaagtg gaagaagaga
     5161 tttcccgaca ctacaggaga gcacacagct gctacaaatg ccgtcagtgc agttttacag
     5221 ctgccgatac tcagtcacta ctggagcact tcaacactgt tcactgccag gaacaggaca
     5281 tcactacagc caacggcgaa gaggacggtc atgccatatc caccatcaaa gaggagccca
     5341 aaattgactt cagggtctac aatctgctaa ctccagactc taaaatggga gagccagttt
     5401 ctgagagtgt ggtgaagaga gagaagctgg aagagaagga cgggctcaaa gagaaagttt
     5461 ggaccgagag ttccagtgat gaccttcgca atgtgacttg gagaggggca gacatcctgc
     5521 gggggagtcc gtcatacacc caagcaagcc tggggctgct gacgcctgtg tctggcaccc
     5581 aagagcagac aaagactcta agggatagtc ccaatgtgga ggccgcccat ctggcgcgac
     5641 ctatttatgg cttggctgtg gaaaccaagg gattcctgca gggggcgcca gctggcggag
     5701 agaagtctgg ggccctcccc cagcagtatc ctgcatcggg agaaaacaag tccaaggatg
     5761 aatcccagtc cctgttacgg aggcgtagag gctccggtgt tttttgtgcc aattgcctga
     5821 ccacaaagac ctctctctgg cgaaagaatg caaatggcgg atatgtatgc aacgcgtgtg
     5881 gcctctacca gaagcttcac tcgactccca ggcctttaaa catcattaaa caaaacaacg
     5941 gtgagcagat tattaggagg agaacaagaa agcgccttaa cccagaggca cttcaggctg
     6001 agcagctcaa caaacagcag aggggcagca atgaggagca agtcaatgga agcccgttag
     6061 agaggaggtc agaagatcat ctaactgaaa gtcaccagag agaaattcca ctccccagcc
     6121 taagtaaata cgaagcccag ggttcattga ctaaaagcca ttctgctcag cagccagtcc
     6181 tggtcagcca aactctggat attcacaaaa ggatgcaacc tttgcacatt cagataaaaa
     6241 gtcctcagga aagtactgga gatccaggaa atagttcatc cgtatctgaa gggaaaggaa
     6301 gttctgagag aggcagtcct atagaaaagt acatgagacc tgcgaaacac ccaaattatt
     6361 caccaccagg cagccctatt gaaaagtacc agtacccact ttttggactt ccctttgtac
     6421 ataatgactt ccagagtgaa gctgattggc tgcggttctg gagtaaatat aagctctccg
     6481 ttcctgggaa tccgcactac ttgagtcacg tgcctggcct accaaatcct tgccaaaact
     6541 atgtgcctta tcccaccttc aatctgcctc ctcatttttc agctgttgga tcagacaatg
     6601 acattcctct agatttggcg atcaagcatt ccagacctgg gccaactgca aacggtgcct
     6661 ccaaggagaa aacgaaggca ccaccaaatg taaaaaatga aggtcccttg aatgtagtaa
     6721 aaacagagaa agttgataga agtactcaag atgaactttc aacaaaatgt gtgcactgtg
     6781 gcattgtctt tctggatgaa gtgatgtatg ctttgcatat gagttgccat ggtgacagtg
     6841 gacctttcca gtgcagcata tgccagcatc tttgcacgga caaatatgac ttcacaacac
     6901 atatccagag gggcctgcat aggaacaatg cacaagtgga aaaaaatgga aaacctaaag
     6961 agacgcgtga attcgatgga tccggggcca ccaacttttc cctgctgaag caggctggcg
     7021 acgtcgagga gaaccctggc cctggctccg gagtgagcaa gggcgaggag gataacatgg
     7081 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     7141 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     7201 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     7261 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     7321 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     7381 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     7441 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     7501 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     7561 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     7621 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     7681 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     7741 tggacgagct gtacaagtga atctagaacg cgttaagtcg acaatcaacc tctggattac
     7801 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     7861 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     7921 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     7981 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     8041 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     8101 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     8161 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     8221 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     8281 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     8341 agtcggatct ccctttgggc cgcttccccg catcgatacc gtcgacctcg agacctagaa
     8401 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     8461 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     8521 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     8581 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     8641 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     8701 tttggatggt gctacaaggc tagcacttct ctagaggagg agtcgtcagt ccagactagc
     8761 agaactagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     8821 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     8881 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     8941 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     9001 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     9061 gctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     9121 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     9181 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     9241 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     9301 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     9361 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     9421 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     9481 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     9541 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     9601 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     9661 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     9721 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     9781 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9841 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9901 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9961 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
    10021 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
    10081 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    10141 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
    10201 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    10261 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    10321 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
    10381 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
    10441 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
    10501 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
    10561 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
    10621 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
    10681 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
    10741 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
    10801 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    10861 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    10921 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    10981 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    11041 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    11101 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    11161 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    11221 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    11281 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    11341 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    11401 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    11461 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11521 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11581 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11641 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11701 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11761 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11821 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11881 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    11941 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12001 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12061 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12121 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    12181 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12241 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    12301 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    12361 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    12421 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    12481 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    12541 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    12601 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    12661 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    12721 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    12781 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    12841 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    12901 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    12961 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13021 ctgatcttca gcatctttta cttttaccag cgtttctggg tgagcaaaaa caggaaggca
    13081 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13141 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13201 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttcc
//
