LOCUS       sequence-413662-       11396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-inducible lentiviral plasmid expressing STAT4-P2A-mCherry, with 
            ORF specific barcode close to 3'LTR site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11396)
  AUTHORS   Obradovic A, Ager C, Turunen M, Nirschl T, Khosravi-Maharlooei M, 
            Iuga A, Jackson CM, Yegnasubramanian S, Tomassoni L, Fernandez EC, 
            McCann P, Rogava M, DeMarzo AM, Kochel CM, Allaf M, Bivalacqua T, 
            Lim M, Realubit R, Karan C, Drake CG, Califano A
  TITLE     Systematic elucidation and pharmacological targeting of 
            tumor-infiltrating regulatory T cell master regulators.
  JOURNAL   Cancer Cell. 2023 May 8;41(5):933-949.e11. doi: 
            10.1016/j.ccell.2023.04.003. Epub 2023 Apr 27.
  PUBMED    37116491
REFERENCE   2  (bases 1 to 11396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2023.04.003"; journalName: "Cancer Cell"; date: 
            "2023-05-8- 8"; volume: "41"; issue: "5"; pages: "933-949.e11"
COMMENT     Sequence Label: Tet-O-FUW-STAT4-P2A-mCherry
FEATURES             Location/Qualifiers
     source          1..11396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(81..100)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        272..651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        653..851
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             869..1049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1096..1221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1714..1947
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2132..2176
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2474..2591
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2648..2666
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2690..2708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2732..2750
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2774..2792
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2816..2834
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2867..2905
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2902..2926
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5280..5336
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5346..6053
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5488..5506)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5756..5775
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6076..6664
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6129..6149)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6547..6558)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6667..6683)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6668..6684)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7235..7415
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7441..7458)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7447..7671
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7717..8145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7804..7823)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8014..8035
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8154..8174)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8159..8488
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8339..8474
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8401..8420
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8536..8583
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8602..8976
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9106..9227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9143..9162)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9197..9216
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9276..9292)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9276..9292)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9289..9311)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9300..9316
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9324..9330,9331..9348,9349..9354))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9369..9390
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9507..9524)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9678..10266)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9758..9777)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10437..11228,11229..11297))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11060..11079
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11298..11396,1..6))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
       61 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
      121 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
      181 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      241 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      301 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      361 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      421 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      481 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      541 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      601 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      661 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      721 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      781 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      841 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
      901 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
      961 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1021 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1081 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1141 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1201 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1261 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1321 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1381 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1441 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1501 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1561 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1621 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1681 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1741 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1801 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1861 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1921 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     1981 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2041 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2101 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2161 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2221 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2281 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2341 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2401 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     2461 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2521 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2581 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta gatctcgagt
     2641 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     2701 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2761 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2821 atcagtgata gagaaaagtg gaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg
     2881 gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca
     2941 tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccccga
     3001 attccgccac catgtctcag tggaatcaag tccaacagtt agaaatcaag tttttggagc
     3061 aggtggatca attctatgat gacaactttc ccatggaaat tcggcatctg ttggcccaat
     3121 ggattgaaaa tcaagactgg gaggcagctt ctaacaatga aaccatggca acgattcttc
     3181 ttcaaaactt gttaatacaa ctggatgaac agttaggtcg tgtttccaaa gagaaaaacc
     3241 tactcttgat acacaatcta aaaagaatta ggaaggtcct tcagggaaaa tttcatggaa
     3301 atccaatgca tgtagctgtg gttatttcaa actgtttaag ggaagagagg agaatattgg
     3361 ctgcagccaa catgcctgtc caggggcctc tagagaaatc cttacaaagt tcttcagttt
     3421 cagaaagaca gaggaatgtg gagcacaaag tggctgccat taaaaacagt gtgcagatga
     3481 cagaacaaga taccaaatac ttagaagatc tgcaagacga atttgactac aggtataaaa
     3541 caattcagac aatggatcag agtgacaaga atagtgccat ggtgaatcag gaagttttga
     3601 cactgcagga aatgcttaac agcctcgatt tcaagagaaa ggaggctctc agtaaaatga
     3661 cccaaatcat ccatgagaca gacctgttaa tgaacaccat gctcatagaa gagctgcaag
     3721 actggaagcg gcggcagcaa atcgcctgca tcgggggtcc actccacaat gggctcgacc
     3781 agcttcagaa ctgctttaca ctattggcag aaagtctttt ccaactgaga aggcaattgg
     3841 agaaactaga ggagcaatct accaaaatga catatgaagg tgatcccatt ccaatgcaaa
     3901 gaactcacat gctagaaaga gtcaccttct tgatctacaa ccttttcaag aactcatttg
     3961 tggttgagcg acagccatgt atgccaaccc accctcagag gccgttggta cttaaaaccc
     4021 taattcagtt cactgtaaaa ctaaggctac taataaaatt gccagaacta aactatcagg
     4081 taaaggttaa ggcatcaatt gacaagaatg tttcaactct aagcaaccga agatttgtac
     4141 tttgtggaac taatgtcaaa gccatgtcta ttgaagaatc ttccaatggg agtctctcag
     4201 tagaatttcg acatttgcaa ccaaaggaaa tgaagtccag tgctggaggt aaaggaaatg
     4261 agggctgtca catggtgact gaagaacttc attccataac gtttgaaaca cagatctgcc
     4321 tctatggcct gaccatagat ttggagacca gctcattgcc tgtggtgatg atttccaatg
     4381 tcagtcagtt acctaatgct tgggcatcca tcatttggta caacgtgtca accaacgatt
     4441 cccagaactt ggttttcttt aataatcctc cacctgccac attgagtcaa ctactggagg
     4501 tgatgagctg gcagttttca tcgtacgttg gtcgtggtct taactcagat caactccata
     4561 tgctggcaga gaagcttaca gtccaatcta gctacagtga tggtcacctc acctgggcca
     4621 agttctgcaa ggaacattta cctggtaaat catttacctt ttggacatgg cttgaagcaa
     4681 tattggatct aattaagaaa cacattcttc ccctttggat tgatgggtat gtcatgggct
     4741 ttgttagcaa agagaaggaa cggctgttgc taaaggataa aatgcctggc acctttttat
     4801 taagattcag tgaaagccat ctcggaggaa taactttcac ctgggtggac cattctgaaa
     4861 gtggggaagt gagattccac tctgtagaac cctacaataa aggccggttg tctgctctgc
     4921 cattcgctga catcctgcga gactacaaag ttattatggc tgaaaacatt cctgaaaacc
     4981 ctctgaagta cctatatcct gacattccca aagacaaagc cttcggtaaa cactacagct
     5041 ctcagccttg cgaagtttca agaccaacag aaaggggtga caaaggttat gttccttctg
     5101 tttttatccc catctcaaca atccgaagtg attcaacaga gccacattct ccatcagacc
     5161 ttcttcccat gtctccaagt gtgtatgcgg tgttgagaga aaacctgagt cccacaacaa
     5221 ttgaaactgc aatgaagtct ccttattctg ctgaaacgcg tgaattcgat ggatccgggg
     5281 ccaccaactt ttccctgctg aagcaggctg gcgacgtcga ggagaaccct ggccctggct
     5341 ccggagtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     5401 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     5461 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     5521 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     5581 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     5641 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     5701 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     5761 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     5821 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     5881 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     5941 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     6001 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tgaatctaga
     6061 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     6121 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     6181 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     6241 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     6301 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     6361 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     6421 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     6481 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     6541 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     6601 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcttcc
     6661 ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     6721 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     6781 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     6841 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     6901 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     6961 acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa ggctagagaa
     7021 ggctcgttaa tgggaaattc gcgcccatct agcagaacta gtaccagttg agcaagagaa
     7081 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     7141 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     7201 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     7261 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7321 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7381 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     7441 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     7501 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     7561 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     7621 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     7681 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     7741 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7801 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7861 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7921 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7981 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8041 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     8101 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     8161 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8221 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     8281 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     8341 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8401 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     8461 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     8521 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     8581 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     8641 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     8701 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     8761 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     8821 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     8881 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     8941 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     9001 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9061 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9121 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9181 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     9241 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     9301 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     9361 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     9421 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     9481 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     9541 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     9601 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     9661 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     9721 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     9781 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     9841 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     9901 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     9961 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10021 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10081 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10141 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10201 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10261 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    10321 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    10381 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    10441 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    10501 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    10561 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    10621 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    10681 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    10741 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    10801 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    10861 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    10921 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    10981 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11041 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11101 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11161 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt taccagcgtt
    11221 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    11281 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    11341 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggt
//
