LOCUS       sequence-413672-        5029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible entry vector, with insert of the TAF5 isoform 
            lacking the alternative exon-8 (∆ex8) mutated to be resistant to 
            TAF5 siRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Xiao MS, Damodaran AP, Kumari B, Dickson E, Xing K, On TA, Parab N, 
            King HE, Perez AR, Guiblet WM, Duncan G, Che A, Chari R, Andresson 
            T, Vidigal JA, Weatheritt RJ, Aregger M, Gonatopoulos-Pournatzis T
  TITLE     Genome-scale exon perturbation screens uncover exons critical for 
            cell fitness.
  JOURNAL   Mol Cell. 2024 Jul 11;84(13):2553-2572.e19. doi: 
            10.1016/j.molcel.2024.05.024. Epub 2024 Jun 24.
  PUBMED    38917794
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2024.05.024"; journalName: "Mol Cell"; date: 
            "2024-07-11- 11"; volume: "84"; issue: "13"; pages: "2553-2572.e19"
COMMENT     Sequence Label: pDONR223-TAF5-∆ex8_WobbleMut
FEATURES             Location/Qualifiers
     source          1..5029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2908..3007)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3024..3043)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3025..3043)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3048..3064)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3048..3064)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3495..4286
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      4379..4967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4868..4887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc gcggcgctgg cggaggagca gacggaggtg gcggtcaagc tagagcctga
      721 gggaccgcca acgctgctac ctccgcaggc gggggacggc gcaggcgagg gtagcggcgg
      781 cactaccaac aacggcccca acggcggcgg cgggaacgtt gcggcgtcgt cgtccactgg
      841 cggggatggc gggaccccca agcccacggt ggctgtctcc gccgctgccc cggcgggggc
      901 ggccccggtg cccgccgctg ctccggacgc cggcgctccg catgaccgac agactctact
      961 ggccgtgctg cagttcctac ggcagagcaa actccgcgag gccgaagagg cgctgcgccg
     1021 tgaggccggg ctgctggagg aggcagtggc gggctccgga gccccgggag aggtggacag
     1081 cgccggcgct gaggtgacca gcgcgcttct cagccgggtg accgcctcgg cccctggccc
     1141 tgcggccccc gaccctccgg gcactggcgc ttcgggggcc acggtcgtct caggttcagc
     1201 ctcaggtcct gcggctccgg gtaaagttgg aagtgttgct gtggaagacc agccagatgt
     1261 cagtgccgtg ttgtcagcct acaaccaaca aggagatccc acaatgtatg aagaatacta
     1321 tagtggactg aaacacttca ttgaatgttc cctggactgc catcgggcag agttgtccca
     1381 acttttttat cctctgtttg tgcacatgta cttggagcta gtctacaatc aacatgagaa
     1441 tgaagcaaag tcattctttg agaagttcca tggagatcag gaatgttatt accaggatga
     1501 cctacgagta ttatctagtc ttaccaaaaa ggaacacatg aaagggaatg agaccatgtt
     1561 ggattttcga acaagtaaat ttgttctgcg tatttcccgt gactcgtacc aactcttgaa
     1621 gaggcatctt caggagaaac agaacaatca gatatggaac atagttcagg agcacctcta
     1681 cattgacatc tttgatggga tgccgcgtag taagcaacag atagatgcga tggtgggaag
     1741 tttggcagga gaggctaaac gagaggcaaa caaatcaaag gtattttttg gtttattaaa
     1801 agaaccagaa attgaggtac ctttggatga cgaggatgaa gagggagaaa atgaagaagg
     1861 aaaacctaaa aagaagaagc ctaaaaaaga tagtattgga tccaaaagca aaaaacaaga
     1921 tcccaatgct ccacctcaga acagaatccc tcttcctgag ttgaaagatt cagataagtt
     1981 ggataagata atgaatatga aagaaaccac caaacgagtg cgccttgggc cggactgctt
     2041 accctccatt tgtttctata catttctcaa tgcttaccag ggtctcactg cagtggatgt
     2101 cactgatgat tctagtctga ttgctggagg ttttgcagat tcaactgtca gagtgtggtc
     2161 ggtaacaccc aaaaagcttc gtagtgtcaa acaagcatca gatcttagtc ttatagacaa
     2221 agaatcagat gatgtcttag aaagaatcat ggatgagaaa acagcaagtg agttgaagat
     2281 tttgtatggt cacagtgggc ctgtctacgg agccagcttc agtccggata ggctctgggc
     2341 tacagaccac tatcagcctt taagaatatt tgccggccat cttgctgatg tgaattgtac
     2401 cagattccat ccaaattcta attatgttgc tacgggctct gcagacagaa ctgtgcggct
     2461 ctgggacgtc ctgaatggta actgtgtaag gatcttcact ggacacaagg gaccaattca
     2521 ttccttgaca ttttctccca atgggagatt cctggctaca ggagcaacag atggcagagt
     2581 gcttctttgg gatattggac atggtttgat ggttggagaa ttaaaaggcc acactgatac
     2641 agtctgttca cttaggttta gtagagatgg tgaaattttg gccagcggca gcatggacaa
     2701 cacagttcga ttatgggatg ctatcaaagc ctttgaagat ttagagaccg atgactttac
     2761 tacagccact gggcatataa atttacctga gaattcacag gagttattgt tgggaacata
     2821 tatgaccaaa tcaacaccag ttgtacacct tcattttact cgaagaaacc tggttctagc
     2881 tgcaggagct tatagtccac aatgataacc cagctttctt gtacaaagtt ggcattataa
     2941 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     3001 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     3061 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     3121 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     3181 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     3241 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     3301 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     3361 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     3421 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     3481 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     3541 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     3601 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     3661 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     3721 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     3781 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     3841 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     3901 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     3961 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     4021 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     4081 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     4141 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     4201 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     4261 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     4321 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4381 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4441 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4501 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     4561 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4621 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4681 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4741 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4801 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4861 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4921 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4981 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgtt
//
