LOCUS       sequence-413679-        6572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV vector plasmid expressing human RAB11 fused to eGFP under a 
            short variant of the CAG (sCAG) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6572)
  AUTHORS   Nieuwenhuis, Eva Labs
  TITLE     Plasmid DNA for mammalian expression and viral vectors
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 6572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: AAV-sCAG-eGFP-RAB11
FEATURES             Location/Qualifiers
     source          1..6572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        254..557
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        559..835
                     /label=chicken beta-actin promoter
     regulatory      980..989
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(986..988,989..991,992..1702)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(986..988,989..991,992..1702)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1031..1052)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1292..1311)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1639..1660
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2465..2586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2476..2495)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2530..2549
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2850..2979
                     /label=AAV2 ITR
     rep_origin      3350..3863
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3437..3456)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3647..3668
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4345..4364)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4464..4486
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4524..4542)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4610..4714
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4715..4783,4784..5575)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4933..4952)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5746..6334
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6235..6254
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcggcctc agtgagcgag
       61 cgagcgcgca gagagggagt ggccaactcc atcactaggg gttccttgta gttaatgatt
      121 aacccgccat gctacttatc tacgtagcca tgctctagac atggctcgac aratcgagct
      181 ccaccgggta ccctagttat taatagtaat caattacggg gtcattagtt catagcccat
      241 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      301 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      361 tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag
      421 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      481 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      541 tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc
      601 ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg
      661 gggcgggggg ggggggggnc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
      721 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
      781 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
      841 cgctgcgacg ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc
      901 ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg
      961 gctgtaatta gcgctgctag ccaccatggt gagcaagggc gaggagctgt tcaccggggt
     1021 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1081 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1141 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1201 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1261 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1321 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1381 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1441 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1501 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1561 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1621 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1681 cggcatggac gagctgtaca agtccggccg gactcagatc tcgagctcaa gcttgattca
     1741 tatgggcacc cgcgacgacg agtacgacta cctctttaaa gttgtcctta ttggagattc
     1801 tggtgttgga aagagtaatc tcctgtctcg atttactcga aatgagttta atctggaaag
     1861 caagagcacc attggagtag agtttgcaac aagaagcatc caggttgatg gaaaaacaat
     1921 aaaggcacag atatgggaca cagcagggca agagcgatat cgagctataa catcagcata
     1981 ttatcgtgga gctgtaggtg ccttattggt ttatgacatt gctaaacatc tcacatatga
     2041 aaatgtagag cgatggctga aagaactgag agatcatgct gatagtaaca ttgttatcat
     2101 gcttgtgggc aataagagtg atctacgtca tctcagggca gttcctacag atgaagcaag
     2161 agcttttgca gaaaagaatg gtttgtcatt cattgaaact tcggccctag actctacaaa
     2221 tgtagaagct gcttttcaga caattttaac agagatttac cgcattgttt ctcagaagca
     2281 aatgtcagac agacgcgaaa atgacatgtc tccaagcaac aatgtggttc ctattcatgt
     2341 tccaccaacc actgaaaaca agccaaaggt gcagtgctgt cagaacatct aaaccggtgg
     2401 atccgatatc aagcttgaat tcgagctagg tacagcttat cgataccgtc gacagcagac
     2461 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     2521 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     2581 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     2641 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgc tcgagggcat gcaacaacaa
     2701 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa
     2761 gtaaaacctc tacaaatgtg gtaaaatccg ataaggacta gagcatggct acgtagataa
     2821 gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc
     2881 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     2941 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tggcgtaata gcgaagaggc
     3001 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gattccgttg
     3061 caatggctgg cggtaatatt gttctggata ttaccagcaa ggccgatagt ttgagttctt
     3121 ctactcaggc aagtgatgtt attactaatc aaagaagtat tgcgacaacg gttaatttgc
     3181 gtgatggaca gactctttta ctcggtggcc tcactgatta taaaaacact tctcaggatt
     3241 ctggcgtacc gttcctgtct aaaatccctt taatcggcct cctgtttagc tcccgctctg
     3301 attctaacga ggaaagcacg ttatacgtgc tcgtcaaagc aaccatagta cgcgccctgt
     3361 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3421 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3481 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3541 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3601 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3661 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     3721 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     3781 aacaaaatat taacgcttac aatttaaata tttgcttata caatcttcct gtttttgggg
     3841 cttttctgat tatcaaccgg ggtacatatg attgacatgc tagttttacg attaccgttc
     3901 atcgattctc ttgtttgctc cagactctca ggcaatgacc tgatagcctt tgtagagacc
     3961 tctcaaaaat agctaccctc tccggcatga atttatcagc tagaacggtt gaatatcata
     4021 ttgatggtga tttgactgtc tccggccttt ctcacccgtt tgaatcttta cctacacatt
     4081 actcaggcat tgcatttaaa atatatgagg gttctaaaaa tttttatcct tgcgttgaaa
     4141 taaaggcttc tcccgcaaaa gtattacagg gtcataatgt ttttggtaca accgatttag
     4201 ctttatgctc tgaggcttta ttgcttaatt ttgctaattc tttgccttgc ctgtatgatt
     4261 tattggatgt tggaatcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4321 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4381 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4441 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4501 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4561 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4621 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4681 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4741 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4801 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4861 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4921 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     4981 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5041 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5101 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5161 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5221 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5281 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5341 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5401 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5461 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5521 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5581 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5641 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5701 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5761 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5821 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5881 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     5941 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6001 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6061 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6121 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6181 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6241 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6301 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6361 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6421 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6481 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6541 ccccgcgcgt tggccgattc attaatgcag ct
//
