LOCUS       sequence-41369-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived 
            from the human PCP2 gene and designed for expression in ON Bipolar 
            cells of the retina..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Korecki AJ, Cueva-Vargas JL, Fornes O, Agostinone J, Farkas RA, 
            Hickmott JW, Lam SL, Mathelier A, Zhou M, Wasserman WW, Di Polo A, 
            Simpson EM
  TITLE     Human MiniPromoters for ocular-rAAV expression in ON bipolar, cone, 
            corneal, endothelial, Muller glial, and PAX6 cells.
  JOURNAL   Gene Ther. 2021 Feb 2. pii: 10.1038/s41434-021-00227-z. doi: 
            10.1038/s41434-021-00227-z.
  PUBMED    33531684
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene Ther. 
            2021 Feb 2. pii: 10.1038/s41434-021-00227-z. doi: 
            10.1038/s41434-021-00227-z."
COMMENT     Sequence Label: pEMS1062
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(90..123)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atgtccatca ggttcttgcg aacctcatca ctcgttgcat cgaccggtaa tgcaggcaaa
       61 ttttggtgta cggtcagtaa attggacatg aagttcctat actttctaga gaataggaac
      121 ttcagatctg agtccggtag cgccacgtga cgcgttggac tccagtcact tttctgctgg
      181 cctctgcccc ggcccagtgc cagagagggc cttttaaacg tccaggacgt gggggtgtgg
      241 attcccccca tccccaaatc cccagctcat gccccatctg ctcccaccca agcttaagcc
      301 ccataaagat catccagata attgtttgcc cacaacgatg ctggatctgg catggtgggt
      361 agggggcagg gcgacctgag cttggacctc gctgtgccca gcagcaatga ctggacccca
      421 gggactctca ggtgtgatgt cacaaaaggc ctgagcagcg tccccacagt tggagaaggg
      481 aggccaacag tgtagggtgg tagctggctt agggaggact tgcaggtgtc cccacccagc
      541 cacccaggtt cccccactgg ctcctggaag ggcagctgcc acctgcagtc ggtaaagaaa
      601 aaggaggttg gagtcaaagg ccccttccct ccctccctct ctgcacctgc ctcagacacc
      661 acccgccacc ctagctccag ctctgtctcc ttggcagcaa gtcaagggg
//
