LOCUS       sequence-413712-       10726 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing the nCas9-AID fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10726)
  AUTHORS   Sharma A, Omer Aden R, Puhar A, Cisneros DA
  TITLE     CRISPR-Cas-Guided Mutagenesis of Chromosome and Virulence Plasmid in
            Shigella flexneri by Cytosine Base Editing.
  JOURNAL   mSystems. 2022 Dec 21:e0104522. doi: 10.1128/msystems.01045-22.
  PUBMED    36541764
REFERENCE   2  (bases 1 to 10726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSystems. 
            2022 Dec 21:e0104522. doi: 10.1128/msystems.01045-22."
COMMENT     Sequence Label: pnCas9-AID
FEATURES             Location/Qualifiers
     source          1..10726
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        199..644
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(645..731,732..2066)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFVKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     primer_bind     complement(2314..2331)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2448..2993)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2528..2547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3054..3713)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3628..3647
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3714..3816)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     4234..4251
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(4352..4975)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(4982..5047)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(5255..5275)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(5276..9349,9350..9352,9353..9379))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(9413..9435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9424..9440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9448..9454,9455..9470,9471..9476))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        9706..9783
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(9803..9822)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg ccggggatca
       61 gccccggatg ctttggatac ggtctatgag ctggcagcgt atttgaccga tccggacacc
      121 tgggataatg tgtggatttt gtcggatcag cttgattagc ggccgcgtat ccgctcaccg
      181 cgatcctttt taacccatca catatacctg ccgttcacta ttatttagtg aaatgagata
      241 ttatgatatt ttctgaattg tgattaaaaa ggcaacttta tgcccatgca acagaaacta
      301 taaaaaatac agagaatgaa aagaaacaga tagatttttt agttctttag gcccgtagtc
      361 tgcaaatcct tttatgattt tctatcaaac aaaagaggaa aatagaccag ttgcaatcca
      421 aacgagagtc taatagaatg aggtcgaaaa gtaaatcgcg cgggtttgtt actgataaag
      481 caggcaagac ctaaaatgtg taaagggcaa agtgtatact ttggcgtcac cccttacata
      541 ttttaggtct ttttttattg tgcgtaacta acttgccatc ttcaaacagg agggctggaa
      601 gaagcagacc gctaacacag tacataaaaa aggagacatg aacgatgaac atcaaaaagt
      661 ttgtaaaaca agcaacagta ttaaccttta ctaccgcact gctggcagga ggcgcaactc
      721 aagcgtttgc gaaagaaacg aaccaaaagc catataagga aacatacggc atttcccata
      781 ttacacgcca tgatatgctg caaatccctg aacagcaaaa aaatgaaaaa tatcaagttc
      841 ctgagttcga ttcgtccaca attaaaaata tctcttctgc aaaaggcctg gacgtttggg
      901 acagctggcc attacaaaac gctgacggca ctgtcgcaaa ctatcacggc taccacatcg
      961 tctttgcatt agccggagat cctaaaaatg cggatgacac atcgatttac atgttctatc
     1021 aaaaagtcgg cgaaacttct attgacagct ggaaaaacgc tggccgcgtc tttaaagaca
     1081 gcgacaaatt cgatgcaaat gattctatcc taaaagacca aacacaagaa tggtcaggtt
     1141 cagccacatt tacatctgac ggaaaaatcc gtttattcta cactgatttc tccggtaaac
     1201 attacggcaa acaaacactg acaactgcac aagttaacgt atcagcatca gacagctctt
     1261 tgaacatcaa cggtgtagag gattataaat caatctttga cggtgacgga aaaacgtatc
     1321 aaaatgtaca gcagttcatc gatgaaggca actacagctc aggcgacaac catacgctga
     1381 gagatcctca ctacgtagaa gataaaggcc acaaatactt agtatttgaa gcaaacactg
     1441 gaactgaaga tggctaccaa ggcgaagaat ctttatttaa caaagcatac tatggcaaaa
     1501 gcacatcatt cttccgtcaa gaaagtcaaa aacttctgca aagcgataaa aaacgcacgg
     1561 ctgagttagc aaacggcgct ctcggtatga ttgagctaaa cgatgattac acactgaaaa
     1621 aagtgatgaa accgctgatt gcatctaaca cagtaacaga tgaaattgaa cgcgcgaacg
     1681 tctttaaaat gaacggcaaa tggtacctgt tcactgactc ccgcggatca aaaatgacga
     1741 ttgacggcat tacgtctaac gatatttaca tgcttggtta tgtttctaat tctttaactg
     1801 gcccatacaa gccgctgaac aaaactggcc ttgtgttaaa aatggatctt gatcctaacg
     1861 atgtaacctt tacttactca cacttcgctg tacctcaagc gaaaggaaac aatgtcgtga
     1921 ttacaagcta tatgacaaac agaggattct acgcagacaa acaatcaacg tttgcgccaa
     1981 gcttcctgct gaacatcaaa ggcaagaaaa catctgttgt caaagacagc atccttgaac
     2041 aaggacaatt aacagttaac aaataaagcg gccgctaatt gcgttgcgct cactgcccgc
     2101 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2161 aggcggtttg cgtattgggc gctagcggag tgtatatact ggcttactat gttggcactg
     2221 atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca
     2281 gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt
     2341 cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc
     2401 caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc
     2461 cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca
     2521 ggactataaa gataccaggc gtttccccct ggcggctccc tcgtgcgctc tcctgttcct
     2581 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttgtctca ttccacgcct
     2641 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
     2701 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
     2761 tgcaaaagca ccactggcag cagccactgg taattgattt agaggagtta gtcttgaagt
     2821 catgcgccgg ttaaggctaa actgaaagga caagttttgg tgactgcgct cctccaagcc
     2881 agttacctcg gttcaaagag ttggtagctc agagaacctt cgaaaaaccg ccctgcaagg
     2941 cggttttttc gttttcagag caagagatta cgcgcagacc aaaacgatct caagaagatc
     3001 atcttattaa tcagataaaa tatttctagg caccaataac tgccttaaaa aaattacgcc
     3061 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     3121 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     3181 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     3241 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     3301 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     3361 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     3421 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     3481 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     3541 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     3601 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta
     3661 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
     3721 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca
     3781 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg
     3841 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct
     3901 tccgtcacag gtatttattc ggcgcaaagt gcgtcgggtg atgctgccaa cttactgatt
     3961 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
     4021 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc acctaggatg cgcttaattt
     4081 gatgcctggc agttccctac tctcgcatgg ggagacccca cactaccatc ggcgctacgg
     4141 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc
     4201 aaattctgtt ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc
     4261 ttctctcatc cgccaaaaca gccaagctga attcgagctc ggtacccggg gatcctctag
     4321 agtcgacctg cagttaacca gagccgcggg acacggcagg ggatttggtg gtgtgcagga
     4381 tcttgacctg gatcatgatg gacagctcgg agcggcgttt ttcggcgcgt ttcagggtct
     4441 tttccagcca gcggttctcg ttgagctggt tgtgggagga ctggatgaag atcttgcggc
     4501 agcactggta gtgttcggac accatcacgt tgagaccgac gccgttatca cgcaggttcc
     4561 acaggccgat ctggttgcgg gcattcttct cgtagtacag cttgcaggcc cagatcttga
     4621 gggtgtggcc gttgccacgg agttcctggt tgtaccactc gaggatcttc tcggcgcagt
     4681 ctgcgcatgg agaccaggag gagtaccagt tgatggtgaa ctggcctggg ttatcgcgca
     4741 ggtattcttc cactttgcgg atggagaaga tctcggcgtg gatgccgcgt tcggtaccgg
     4801 attgtggctt gttcactgcg taaccccaga agcatgcgcg acgttcaccg cggcgcttca
     4861 gttcgaacag cacgtagcag cggtgggaca cggatttctt gttgttgaag aactgcttct
     4921 tgaaggtgta gatgtccagc ttttcgtgga tgcgcacgta ctcggcgtcg gtcatgcggg
     4981 atttatcgtc gtcgtcctta tagtcgatgt cgtgatcctt ataatcgcca tcatggtcct
     5041 tgtaatcgcc ggagtacttc tcgacgtatg gcacagggat catgccgcgc ttaccttcgg
     5101 agtcttcggc gttccaccac tgttcctcag gcttatcacg gatgcggagg atatcgccct
     5161 tcttgaatgg cagatcctcc tcatcgttgc cgttaaagtc gaacagggcg cgcacgtatt
     5221 ctgcagaacc gccaccgcca gagccaccac caccgacctt gcgtttcttc tttgggtcac
     5281 ctcctagctg actcaaatca atgcgtgttt cataaagacc agtgatggat tgatggataa
     5341 gagtggcatc taaaacttct tttgtagacg tatatcgttt acgatcaatt gttgtatcaa
     5401 aatatttaaa agcagcggga gctccaagat tcgtcaacgt aaataaatga ataatatttt
     5461 ctgcttgttc acgtattggt ttgtctctat gtttgttata tgcactaaga actttatcta
     5521 aattggcatc tgctaaaata acacgcttag aaaattcact gatttgctca ataatctcat
     5581 ctaaataatg cttatgctgc tccacaaaca attgtttttg ttcgttatct tctggactac
     5641 ccttcaactt ttcataatga ctagctaaat ataaaaaatt cacatatttg cttggcagag
     5701 ccagctcatt tcctttttgt aattctccgg cactagccag catccgttta cgaccgtttt
     5761 ctaactcaaa aagactatat ttaggtagtt taatgattaa gtctttttta acttccttat
     5821 atcctttagc ttctaaaaag tcaatcggat ttttttcaaa ggaacttctt tccataattg
     5881 tgatccctag taactcttta acggatttta acttcttcga tttccctttt tccaccttag
     5941 caaccactag gactgaataa gctaccgttg gactatcaaa accaccatat ttttttggat
     6001 cccagtcttt tttacgagca ataagcttgt ccgaatttct ttttggtaaa attgactcct
     6061 tggagaatcc gcctgtctgt acttctgttt tcttgacaat attgacttgg ggcatggaca
     6121 atactttgcg cactgtggca aaatctcgcc ctttatccca gacaatttct ccagtttccc
     6181 cattagtttc gattagaggg cgtttgcgaa tctctccatt tgcaagtgta atttctgttt
     6241 tgaagaagtt catgatatta gagtaaaaga aatattttgc ggttgctttg cctatttctt
     6301 gctcagactt agcaatcatt ttacgaacat cataaacttt ataatcacca tagacaaact
     6361 ccgattcaag ttttggatat ttcttaatca aagcagttcc aacgacggca tttagatacg
     6421 catcatgggc atgatggtaa ttgttaatct cacgtacttt atagaattgg aaatcttttc
     6481 ggaagtcaga aactaattta gattttaagg taatcacttt aacctctcga ataagtttat
     6541 cattttcatc gtatttagta ttcatgcgac tatccaaaat ttgtgccaca tgcttagtga
     6601 tttggcgagt ttcaaccaat tggcgtttga taaaaccagc tttatcaagt tcactcaaac
     6661 ctccacgttc agctttcgtt aaattatcaa acttacgttg agtgattaac ttggcgttta
     6721 gaagttgtct ccaatagttt ttcatctttt tgactacttc ttcacttgga acgttatccg
     6781 atttaccacg atttttatca gaacgcgtta agaccttatt gtctattgaa tcgtctttaa
     6841 ggaaactttg tggaacaata tgatcgacat cataatcact taaacgatta atatctaatt
     6901 cttggtccac atacatgtct cttccatttt ggagataata gagatagagc ttttcatttt
     6961 gcaattgagt attttcaaca ggatgctctt taagaatctg acttcctaat tctttgatac
     7021 cttcttcgat tcgtttcata cgctctcgcg aatttttctg gcccttttga gttgtctgat
     7081 tttcacgtgc catttcaata acgatatttt ctggcttatg ccgccccatt actttgacca
     7141 attcatcaac aacttttaca gtctgtaaaa tacctttttt aatagcaggg ctaccagcta
     7201 aatttgcaat atgttcatgt aaactatcgc cttgtccaga cacttgtgct ttttgaatgt
     7261 cttctttaaa tgtcaaacta tcatcatgga tcagctgcat aaaattgcga ttggcaaaac
     7321 catctgattt caaaaaatct aatattgttt tgccagattg cttatcccta ataccattaa
     7381 tcaattttcg agacaaacgt ccccaaccag tataacggcg acgtttaagc tgtttcatca
     7441 ccttatcatc aaagaggtga gcatatgttt taagtctttc ctcaatcatc tccctatctt
     7501 caaataaggt caatgttaaa acaatatcct ctaagatatc ttcattttct tcattatcca
     7561 aaaaatcttt atctttaata atttttagca aatcatggta ggtacctaat gaagcattaa
     7621 atctatcttc aactcctgaa atttcaacac tatcaaaaca ttctattttt ttgaaataat
     7681 cttcttttaa ttgcttaacg gttacttttc gatttgtttt gaagagtaaa tcaacaatgg
     7741 ctttcttctg ttcacctgaa agaaatgctg gttttcgcat tccttcagta acatatttga
     7801 cctttgtcaa ttcgttataa accgtaaaat actcataaag caaactatgt tttggtagta
     7861 ctttttcatt tggaagattt ttatcaaagt ttgtcatgcg ttcaataaat gattgagctg
     7921 aagcaccttt atcgacaact tcttcaaaat tccatggggt aattgtttct tcagacttcc
     7981 gagtcatcca tgcaaaacga ctattgccac gcgccaatgg accaacataa taaggaattc
     8041 gaaaagtcaa gattttttca atcttctcac gattgtcttt taaaaatgga taaaagtctt
     8101 cttgtcttct caaaatagca tgcagctcac ccaagtgaat ttgatgggga atagagccgt
     8161 tgtcaaaggt ccgttgcttg cgcagcaaat cttcacgatt tagtttcacc aataattcct
     8221 cagtaccatc cattttttct aaaattggtt tgataaattt ataaaattct tcttggctag
     8281 ctcccccatc aatataacct gcatatccgt tttttgattg atcaaaaaag atttctttat
     8341 acttttctgg aagttgttgt cgaactaaag cttttaaaag agtcaagtct tgatgatgtt
     8401 catcgtagcg tttaatcatt gaagctgata ggggagcctt agttatttca gtatttactc
     8461 ttaggatatc tgaaagtaaa atagcatctg ataaattctt agctgccaaa aacaaatcag
     8521 catattgatc tccaatttgc gccaataaat tatctaaatc atcatcgtaa gtatcttttg
     8581 aaagctgtaa tttagcatct tctgccaaat caaaatttga tttaaaatta ggggtcaaac
     8641 ccaatgacaa agcaatgaga ttcccaaata agccattttt cttctcaccg gggagctgag
     8701 caatgagatt ttctaatcgt cttgatttac tcaatcgtgc agaaagaatc gctttagcat
     8761 ctactccact tgcgttaata gggttttctt caaataattg attgtaggtt tgtaccaact
     8821 ggataaatag tttgtccaca tcactattat caggatttaa atctccctca atcaaaaaat
     8881 gaccacgaaa cttaatcata tgcgctaagg ccaaatagat taagcgcaaa tccgctttat
     8941 cagtagaatc taccaatttt tttcgcagat gatagatagt tggatatttc tcatgataag
     9001 caacttcatc tactatattt ccaaaaatag gatgacgttc atgcttcttg tcttcttcca
     9061 ccaaaaaaga ctcttcaagt cgatgaaaga aactatcatc tactttcgcc atctcatttg
     9121 aaaaaatctc ctgtagataa caaatacgat tcttccgacg tgtatacctt ctacgagctg
     9181 tccgtttgag acgagtcgct tccgctgtct ctccactgtc aaataaaaga gcccctataa
     9241 gatttttttt gatactgtgg cggtctgtat ttcccagaac cttgaacttt ttagacggaa
     9301 ccttatattc atcagtgatc accgcccatc cgacgctatt tgtgccgata gctaagccta
     9361 ttgagtattt cttatccatt ctcaactcct ttaagcttaa ttaattctgt ttcctgtgtg
     9421 aaattgttat ccgctcacaa ttccacacat tatacgagcc gatgattaat tgtcaacagc
     9481 tcatttcaga atatttgcca gaaccgttat gatgtcggcg caaaaaacat tatccagaac
     9541 gggagtgcgc cttgagcgac acgaattatg cagtgattta cgacctgcac agccatacca
     9601 cagcttccga tggctgcctg acgccagaag cattggtgca ccgtgcagtc gataagcccg
     9661 gatcagcttg caattcgcgc gcgaaggcga agcggcattt acgttgacac catcgaatgg
     9721 tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg
     9781 aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
     9841 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
     9901 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
     9961 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
    10021 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
    10081 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc
    10141 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
    10201 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
    10261 attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
    10321 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
    10381 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac
    10441 tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc
    10501 actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag
    10561 tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc
    10621 tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc
    10681 agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagg
//
