LOCUS       sequence-413719-       11846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prig-3 FRT let858 (stop) FRT twk-40(gf)::GFP unc-54 3' UTR C.elegans
            AVA and other neurons expression of twk-40 (gf ) GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11846)
  AUTHORS   Meng J, Ahamed T, Yu B, Hung W, Ei Mouridi S, Wang Z, Zhang Y, Wen 
            Q, Boulin T, Gao S, Zhen M
  TITLE     A tonically active master neuron modulates mutually exclusive motor 
            states at two timescales.
  JOURNAL   Sci Adv. 2024 Apr 12;10(15):eadk0002. doi: 10.1126/sciadv.adk0002. 
            Epub 2024 Apr 10.
  PUBMED    38598630
REFERENCE   2  (bases 1 to 11846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-04-12"; volume: "10(15):eadk0002. doi"; pages: " 
            10.1126/sciadv.adk0002. Epub 2024 Apr 10"
COMMENT     Sequence Label: pJH4203
FEATURES             Location/Qualifiers
     source          1..11846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(4865..4898)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(5380..5413)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7523..7551)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     8299..8319
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(9286..9308)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9408..9427
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9505..9523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9591..9695
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9696..9764,9765..10556)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9914..9933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10727..11315
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11216..11235
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11469..11486
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11603..11624
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11639..11644,11645..11662,11663..11669)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11677..11693
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11682..11704
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11701..11717
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11701..11717
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagcatgc ggccgctcgt ctttaccgct ttgtttttta ttgcttactt
       61 tacctctagt gattatctta ttggaattta tgcacatgac gtgttgtact agtttaacaa
      121 tttcaattga cagttttttc gattaggctt ttgaacatat ttatatattt ctttatttac
      181 gaatgtgaac ttaaaagtgg attctcgaaa tcttttatcg ggttttttga gcctaaaatg
      241 acccatttat caagatttat caaaaaagct atgacggttt aaaggttttt gattaaaggc
      301 gtgttttttt tagctaaaat ctcaaactct gctaaactat cagtgcccca acgtgaaaaa
      361 acaacatgaa aaaccccaaa atcaaaaagc atcttaatca gccaaaaaca cttcataaat
      421 tgcaaaaata tgcattaaca aatgacaact aaatggaaaa gtactcccga cttttcaaga
      481 tggaaaaatg tgagatcttc gctgaaaata gagttttttt tcaaatgaaa aatttggaat
      541 aaagctcaaa tgtttgtctc aaagttggta attgttgaaa aaaattttga taatttggcg
      601 aatatttgtg aattgaaaca taactatttc ggttttacgc gtacttgtaa aattcaaaat
      661 tttacaagaa attataaaac tttatgagct ttagatggtc aatacgcaaa cattcatcta
      721 gtttatagcc ttacattaaa acattttacc tattcaggtt aattgaacag tatctttaaa
      781 ggtggtgtag tcgaattttt taaaatttct ttattagact caaaattgtc tgaaaacacc
      841 aaatttcata agtaaacttc ttgaaaactt ttcggctcga aaaaatgacc taaaattagt
      901 gaaaatttga aatgtggcca atttatcaag cggctgcaaa caattttttt ttgaaattgt
      961 tgtctgattt tggatataga tgtaaggtat ttaaaaaaaa gatggacgaa gaaaattggt
     1021 aaaaaaattt gacaaatctc ttcgtccatc gactttgaaa gaccttaatc aagtttaaaa
     1081 cgcaagatag tcaatttata tacccaaaat cagatgataa ttcaaaaaaa attgtttcca
     1141 gccgctgcga caatgttaag ttaatttaag gtcatttttc gagcagccat aacttctttt
     1201 ttttaagttt caacaagttt cattataaaa ttcggtgttt tcagacaatt ttgaatataa
     1261 taaagaaata aagaaaaatt cgactacatc acctttaaac taaatttcaa atgtaatttt
     1321 tttttacttt tcaaaatgat tcgttctcgg ttttgttgtg aacatttttt tgctaatacg
     1381 cttacgaaat gttttgtgtg gcaatcattt tgattgaagt tcccggcagc tacaacatat
     1441 ttttagaact agaaaacaat agaacgcaca gaatatccac gttcccatgt tttcaatttc
     1501 tcttttcgaa atcctttaca aaatccgaac gccgttctga ttcagccttg agacattttt
     1561 caaaaaaaaa atcagtgtga gcattatagt tttcagtttt gaatattcac attttgcact
     1621 ttttgcctac tttccgtatt ctgcagtttc cacatcacct tgttacatct ctcaggaagt
     1681 ttctgaaagt gacaccacgc tcacacacga tgaaattcat acccaccaac ctcccttctc
     1741 tgcctccctc aacttcttcc acggaattct atctcctctt atccagttag ggacacatat
     1801 cgagtttcac caaaaataat gagaagtgtg gtcagagaca ccacattgaa ttaactatta
     1861 gttcaaaagt tgcaggtttc ctagtgcaaa aagtttatgt tcccatctac caaacgactc
     1921 accgccaatt gactttgcag tggcaacatt gggaaatgcg acccatcata ttgttcctat
     1981 tgtcatgtac aagctgtccc tgttttgctc gcttaatccg tcaacccctt ccccgttcta
     2041 attagagatg agtgaattac aatagagttg aaacggaaat ctgagtgtct gtagctttcc
     2101 gccgccgctt ttgttacgag atggtagacg tccctttctt ggtgtttatt gtctcacaaa
     2161 ctgtataata aagcaccgtt gtgtaaaagg catttggtat caggaagata aaatgaataa
     2221 aatgcaaatg cggaatcaaa aattgccaaa taattttgac caataaaata tcaaaaataa
     2281 tagaacagaa ataaacaaat aaaactacgg agaaaatgtg attattccat taaacaacgt
     2341 tttaaaccaa ctttattcgt cagtggggaa ggttttggac aaatatcata gtaaaacttg
     2401 ttcgttttaa ttatacataa tcaataatta taggtgttag atccaatggt cgaaatttaa
     2461 aaaaaaatca acattttaga aaatttcggc aatttaccga aatattgact gacaatttta
     2521 catatataag tgttttgaaa tagaatcccc attgacgatg cgccacctta aacagtagaa
     2581 ctgttccaat cagaaactaa ataaatacgg ttattatata tatatatata tatatataac
     2641 ttgtttttat tatgcatttc tcaaaactca ccaaactcaa tctctaattt acggttgttg
     2701 aggaaaatat atttatcaca tttagttgag actctaaaag aagtacaact tttttcttgc
     2761 aattcctcca ccaatccaat ttttcaagag tcaattgctt ggagtcctcg ttttctcgta
     2821 atccgatatt ccccctattt ctgtgatttt attcagtttc gtcctcttac accaaaaatc
     2881 ttcatgactt ttttggtttt ctgcttaatc ttttttttgt ttttaaagtc taccatccgg
     2941 caattttttt tgcaaatggt tgctcagaat ttgacctcag attaaaattt tcaggaactt
     3001 cctggtccca tttcgtcaca tttaataaca aaccaaatcc ttcagcttga gtcgtccaag
     3061 ccaacgcaac cctaattcga ttccacctcc aatttacctt tttgccgttt tcgatcaaat
     3121 tgtgggggct ctttttattt gaaagtcgta aaatggcgcg tccttgtcga aaattgagat
     3181 agctaatggt tccaaataaa atcaaatgca tgaacctagt aggaaaaatc gttttttttt
     3241 tcatcttcca aagaaaaagt ttaagataaa cccaccactc cagcaatcca cctacgcgtt
     3301 tttcaccgga aactgtcatt tgtttttgat gtatgacagt gccagcactg aactaaattc
     3361 ccatttgttt tgttgtggtg ttatctaacc acgtggctct ttctctaaat tttgcgtagg
     3421 ctgcaagatc cgcaatccca ccaaaaaatg gtttctaatg tacttcatac atatcccaca
     3481 tgactcagtg ctccgaaacg cccagcaacc cccgcgtttg tctttacaaa aatgaggcgg
     3541 aaatgggaaa ttttcctgct attgctatgg taaaatactt aagctgtatt gagatttggg
     3601 aaattgaaag ataagatgaa aaatccactc tgtataatta aaaaggttta ttttttttgt
     3661 aatttttgta ccgcaaattt tttcattaac tgatttttct ttattcattc gatggcccag
     3721 tttcagatct tcattatcat gtagtttatt cattcaaact atttgtgaca tcatgatggt
     3781 tgtttaaaaa taatttcgaa tgaagaaagc ttagacctat tataattttc ttttctataa
     3841 aaaatattga ttttgtaaag cgtttggcag tgacagtacc aaaaaatgtt ttaaaatgat
     3901 ttaagaaaat tttttatttt atttgtataa tacgcgtaaa atgagtatac tcgtcatttg
     3961 tcacgaatcg agttctcagt ttgctgtgaa tatcttgtaa tagaaatcga tctcatagtg
     4021 tcacttttga ggcataactt ttcaggttgt tacaagttca ttaacttgtc aagactgggt
     4081 catgttttaa agtgtaactg ttacccatgt accctaaact ttgagacaaa ctcctcaaaa
     4141 cttgtaacca gaatcctatg tagatgactc acaaaaccct gttttcaatt tgaacgaatt
     4201 tcgaaactgg ccaaaatgtt cccctcttgc tcacctaatg cttttaccat ttaaaaagga
     4261 tggtaacaac actctttcga aaatgtaggc ggtccatttt cggtttctca taaccgtctg
     4321 gctctatctc ctagtgcgcg aggagagggc gggcggagta ggaatcgggg gcagcgcact
     4381 catacactct gcccttctct tttgcacaca cagaaaaaga tagatacaga ccaacactta
     4441 ttgttgtcca gtaagcttcc agaaaactat tttcagaatt acaaactaca tttttctaga
     4501 acagtcaatt gattttttac aagattcgag tattctaaca ttagtgaaag tatttagcca
     4561 ctgcaatttt caactgtagg tttcaacttc caaaaaaaat tcctggcatt tctaattgga
     4621 tgaaaaaagg ctatctagac cctccattat ttactcataa tttcatctcg tcctcatttt
     4681 taatcccctc attgatccca tcacaatccg ccaaaatgtt ttttaaacac ttttttcttc
     4741 accagccgta tgtggtcaac tgcctaaaaa tttcacagct atttgtcatt ccttgcagaa
     4801 gaacttcatt cttctttttc gaaaatcccg ggattggcca aggtaagttt aaacagttcg
     4861 gtacgaagtt cctatacttt ctagagaata ggaacttcta acataacata gaacattttc
     4921 agggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt
     4981 ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa aaaattaatt
     5041 caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat tccgctcctc
     5101 tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat taggacgtgg
     5161 ggggaattgg tggaaggggg aaacacacaa aaggatgatg gaaatgaaat aaggacacac
     5221 aatatgcaac aacattcaat tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat
     5281 atatagagga ggaaggaaaa ctagtaaaaa ataagcaaag aaattaggcg aacgatgaga
     5341 attgtcctcg cttggaggta agtttaaaca gttcggtacg aagttcctat actttctaga
     5401 gaataggaac ttctaactaa ccatacatat ttaaattttc agggctagca tggcaacatg
     5461 gaaaacctac gcccggataa tcctcgctca cgtcagtctt attgtcttgt cggttgtcta
     5521 tgttggattt ggagcgtttc tcttctatca gttggagcaa ccgaatgaag ttgaagttcg
     5581 agctaggaat atcgaaaggt tcaatattca caaacgacaa atgattgaac atttatggga
     5641 gatgagggaa agcggtattg gacaacacgt ggtggaggat ctggctgtaa aatacgtaga
     5701 caacgtcact cgcattcttt tcgaggcatt tgacacccat tgtataggag ctaagcatct
     5761 gagacccggc ggagaggatg aagacgtgag ttggagtctg aaatgtttat ctgaaattga
     5821 atttgcactg tgtttaagtt tcacaaactc aatttttaaa tttcagtaca attggaccta
     5881 catgacagct ttatttttca ctacaactct cctcacgact attggttacg gtaacttgac
     5941 cccagtaacc ggaagaggaa aattgctctg catcctatac gcacttttcg gtgtcccact
     6001 gatcaatata actgttgccg atatcggaaa gtttttatct gaaaatattg ttcaattgta
     6061 tacttggtga gacacttttt tttgaaaact tttttaaaaa tccgaaattc aggtaccgaa
     6121 agcttcggga aaagtgctca aaacaaaaat acagtgtcat ttccagtaag gacgataaga
     6181 ataaggaagg ggacctgaat ctggtaagag ggtctaaaaa tggtctagta gtggttaaaa
     6241 tgggcaaaaa caggcaaaca ttttttgaaa ttaataagta catttcgagt atacttttga
     6301 aatcagccaa tatcgggtac atttttaaaa ttcggaaaaa aaaacgagcg ataaaaccgc
     6361 ccgctaatca ccggcaactt gcccatcttt cagcttctac aaaaaattca atttctccag
     6421 taaaccaaaa aaaaaacttg aaaaaaaaac aaaacatttt gttttttttt cttttttggt
     6481 ctgaacaaaa aactaaaaaa aaacatccaa aaaattcatt tttgccaaaa acaccaaaat
     6541 aaccaaaaca atcgaccact tcaccctcga acactttcca attagaactt tttaaggatc
     6601 atttggaaaa ctacatctcg attcctatat tcctaattgt ggcgatacta ctcagctaca
     6661 taacatttgg agcagttgtt ctgagtatgt gggaaggatg ggatttcttc tctggattct
     6721 actttagctt tattacaatg acgactgtcg gatttggtga tatagtacca ttgaaacggg
     6781 aatattatat tttagactta tgctatatta ttattggatt atcgattacg taagtttgtg
     6841 attttggcta ttagtgcctc gagtctcaaa aaaactgtag tttgtagttg acaaatttca
     6901 aatcgttgaa aatttttgcc agttgcgtta ttttatatag gtagtaccaa caaaaaattt
     6961 ccagcacgat gtgtatcgat ttggttggaa ttcagtatat tcggaaaatg cattacttcg
     7021 gaagagctat taaggtaaga agctaaattt ttagtcctac taaaattttc taggacgccc
     7081 gatttgcttt ggtaaacgtc ggcgggaaga tggttcacgt gccggattta atgagatatg
     7141 cgagtgttct acaacaaaag tatggccaaa agaagaccca tgacacaata attgtcaagg
     7201 gagcttatgc tccaaaggat ctgagcaaaa tacggtgaga ggactcggtt tttttcttaa
     7261 taaaaagaaa aatcaattta aatttttgta tatctagatt cgtataacag ttcagttaca
     7321 aattaaatat ttcaaatatt catttggtat ttactctgat aagttttgat atctgaaata
     7381 ttttgaaaac tcgtaaattt ccagatttat cgattttgga gcattggcct caattgaaag
     7441 ttggcaatcg ctatggtcaa ttctcactgg aagaactcaa gaagttcatg ttatgagtaa
     7501 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     7561 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     7621 ccttaaattt atttgcacta ctggaaaact acctgttcca tgggtaagtt taaacatata
     7681 tatactaact aaccctgatt atttaaattt tcagccaaca cttgtcacta ctttctgtta
     7741 tggtgttcaa tgcttctcga gatacccaga tcatatgaaa cggcatgact ttttcaagag
     7801 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     7861 caagacacgt aagtttaaac agttcggtac taactaacca tacatattta aattttcagg
     7921 tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga
     7981 ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa
     8041 tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttgtaagtt taaacatgat
     8101 tttactaact aactaatctg atttaaattt tcagaacttc aaaattagac acaacattga
     8161 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     8221 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
     8281 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     8341 catggatgaa ctatacaaat agcattcgta gaattccaac tgagcgccgg tcgctaccat
     8401 taccaacttg tctggtgtca aaaataatag gggccgctgt catcagagta agtttaaact
     8461 gagttctact aactaacgag taatatttaa attttcagca tctcgcgccc gtgcctctga
     8521 cttctaagtc caattactct tcaacatccc tacatgctct ttctccctgt gctcccaccc
     8581 cctatttttg ttattatcaa aaaacttctc ttaatttctt tgttttttag cttcttttaa
     8641 gtcacctcta acaatgaaat tgtgtagatt caaaaataga attaattcgt aataaaaagt
     8701 cgaaaaaaat tgtgctccct ccccccatta ataataattc tatcccaaaa tctacacaat
     8761 gttctgtgta cacttcttat gttttttact tctgataaat ttttttgaaa catcatagaa
     8821 aaaaccgcac acaaaatacc ttatcatatg ttacgtttca gtttatgacc gcaattttta
     8881 tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg ctcatcgtga aaaagttttg
     8941 gagtattttt ggaatttttc aatcaagtga aagtttatga aattaatttt cctgcttttg
     9001 ctttttgggg tttcccctat tgtttgtcaa gatttcgagg acggcgtttt tcttgctaaa
     9061 atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg gtttgaggct
     9121 cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat ggggtttttg
     9181 ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta ctagtcggcc
     9241 gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9301 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     9361 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     9421 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     9481 ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag gttaatgtca
     9541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
    10021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
    10081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
    10141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
    10201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
    10261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
    10321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
    10381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
    10441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
    10501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
    10561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    11041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    11101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    11161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    11221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    11281 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    11341 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    11401 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    11461 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    11521 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    11581 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11641 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11701 caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac
    11761 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
    11821 atacgctaac aacttggaaa tgaaat
//
