LOCUS       sequence-41380-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1111
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(660..671)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctcctcgccc ttgctcacca tggtggcggc gaagttccta taccttttga agaataggaa
       61 cttcggcgcg cctgagggcg ggcatggctg gggtgagctc tctggtccca cctggccatt
      121 tatgccctgg tcctgaatgg ggtgggtcca ggcagcacgg gtaatcacat ttgtctagcg
      181 gggaatccaa ttttccctcc cacaagcttg cgccccaggc caagcactca gcctgctgcc
      241 ctcctctaat tggcactaat gacacatgga cgtcatcagt gggagttgaa gtagacaggc
      301 ggatgctgtc ccttctgaga tggctctgaa gagcccgttt gtacaatact agcaggtggg
      361 cccatttcaa accaaaaata agacaatctc caaatagtcc ctgtgtttag acactctctc
      421 cacggcctgc tcttcccact tttgtctcct ctcaattttt ccacagcctc cagctggtac
      481 cactgctgct ccagaaggcg cgagagcatc atagggagtg ggggacatgg gcgtggggtg
      541 cattgctggg gacagacgga gctggagctg cacaggggat aggcaggctg cagggtgcac
      601 tgctgggggt gacagccttt aaggaaaaga gggtgagtga caggcctgct cccctgtctc
      661 tgaagctccc aaacccacag cttcctcttg cccagagcag atctggcctg aatttgaagc
      721 ctctcagggc agctggccgc agccctgctt ctcccacacc tgccacagga cctttgccat
      781 catccacccg tgggcccgag ggtcagtctc accac
//
