LOCUS       sequence-413876-       12598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1352
FEATURES             Location/Qualifiers
     source          1..12598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8455..9043
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8508..8528)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8926..8937)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9115..9348
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9426..9547
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9463..9482)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9517..9536
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9587..9722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9742..9761)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9743..9761)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9771..9788)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9771..9787)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9780..9802)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9929..10384
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10016..10035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10226..10247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10410..10514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10515..10583,10584..11375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10733..10752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11546..12134
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12035..12054
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12288..12305
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12422..12443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12458..12463,12464..12481,12482..12488)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12496..12512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12501..12523
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12520..12536
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12520..12536
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12555..12575
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12557..12575
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gacaccgacc tgttcatgga
     4141 atgtgaggag gaggagttgg agccatggca gaaaatcagt gatgtcattg aggactctgt
     4201 agttgaagat tataattcag tggataaaac taccacagtt tctgtgagcc agcagccagt
     4261 ctcggctcca gtgcccatcg ctgcccatgc ttctgttgct gggcacctct ctacatccac
     4321 caccgttagt agcagcgggg cacagaacag cgacagtaca aagaagactc ttgtcacact
     4381 aattgccaac aacaatgctg gcaatccttt ggtccagcaa ggtggacagc cactcatcct
     4441 gacccagaat ccagccccag gtctgggcac aatggttact caaccagtat tgaggcctgt
     4501 tcaggtcatg cagaatgcca atcatgtgac tagttcccct gtggcctcac aaccaatatt
     4561 tatcactacg cagggatttc ctgtaaggaa tgtccggcct gtacaaaatg caatgaatca
     4621 ggttgggatt gtgctgaacg tacagcaagg ccaaacggtt agaccaatta cactagttcc
     4681 agccccaggt acccagtttg ttaagccgac agttggagtt ccacaagtgt tctcccagat
     4741 gacccctgtg aggccaggct ccacaatgcc tgtgaggccc accaccaaca ccttcaccac
     4801 cgtcatcccg gccactctta ccattcgaag caccgtccca cagtcccagt cccagcagac
     4861 caagtccact cccagcactt ctaccactcc cactgccaca cagccaacct cactggggca
     4921 actagctgtt cagtctccag gccagtcaaa ccagaccacg aatcccaagc tagctccctc
     4981 cttcccctct ccacctgcag tgagcattgc cagctttgtc actgtgaagc gacctggtgt
     5041 tacaggcgaa aatagcaatg aagtggccaa attggtgaat acccttaaca ccatcccttc
     5101 cctgggccag agtcctgggc cagtggtggt gtccaacaac agctctgctc atggctctca
     5161 aagaaccagc ggacctgagt cttcaatgaa agtgacctct tccatcccag tatttgacct
     5221 ccaggatggt ggacggaaaa tatgtccacg atgtaatgct caatttcgtg ttactgaagc
     5281 tttgagaggt cacatgtgtt actgttgccc agaaatggtt gaataccaga agaaaggaaa
     5341 gtccctggat tcagaaccca gtgtcccatc agcagcaaag cccccatccc ctgagaaaac
     5401 agctcctgtt gcttccacac cctcttctac acctattcct gctctgtcac cgcctaccaa
     5461 agtaccagaa ccaaatgaga acgtgggcga tgccgtccag accaaactca ttatgcttgt
     5521 agatgacttc tactatggac gggatggtgg caaagtagcc cagctcacaa atttccctaa
     5581 ggtcgccaca tctttccgat gcccacattg taccaaaagg ctaaaaaaca atattcgatt
     5641 catgaaccat atgaaacacc acgtagaact cgatcagcag aacggtgagg tagatggtca
     5701 cactatctgc cagcactgtt accgccagtt ttccactccc ttccagcttc agtgccactt
     5761 ggaaaatgtt catagtccct atgaatctac taccaagtgc aagatctgtg aatgggcgtt
     5821 tgaaagtgag ccactatttc tccagcatat gaaggatact cataagcctg gagagatgcc
     5881 ttatgtttgc caggtgtgtc aatatcgctc ctcactctac tctgaggtag atgtccattt
     5941 tcggatgatc catgaggata cccggcatct gctctgccct tattgcctga aggtcttcaa
     6001 aaatggcaat gcattccaac agcattacat gaggcaccag aagagaaatg tttatcactg
     6061 caacaaatgc cggctgcagt ttctctttgc caaggacaaa attgaacaca agcttcaaca
     6121 ccataaaacc ttccgtaaac ccaagcagct ggagggcttg aaaccaggca ccaaggtgac
     6181 aatccgggct tcccgagggc agccacgaac tgttcctgta tcctctaatg atacacctcc
     6241 cagcgccttg caggaggcag caccgctgac ctcctcaatg gaccctctgc ctgtcttcct
     6301 ttatccccct gtccagcgca gcatccagaa gagagctgtt aggaaaatga gtgtcatggg
     6361 ccggcagaca tgcctggagt gcagcttcga gatcccagac ttccctaatc atttccctac
     6421 ttacgtacac tgctctctgt gtcgctatag cacctgctgt tctcgagctt atgccaacca
     6481 catgatcaac aatcatgttc cacggaagag ccccaagtat ttggctttgt ttaaaaattc
     6541 tgtgagtgga atcaagctgg cctgcacttc atgtaccttt gttacctctg tgggcgatgc
     6601 tatggccaag catttggtat tcaacccctc tcacagatcc agcagcatcc tgccacgggg
     6661 actcacttgg atagctcact caaggcatgg ccagactcgt gaccgagtgc atgaccggaa
     6721 cgtgaagaat atgtaccctc ctccttcctt ccccactaac aaagctgcca ctgtgaaatc
     6781 tgcgggggcc accccagctg agcctgaaga gctactaact cccttagccc cagcactccc
     6841 atcaccagcc tcaactgcaa ccccaccacc aacccccact cacccgcagg ctttagccct
     6901 tccaccgctg gctacagagg gagccgaatg tctgaatgtt gatgatcagg atgaagggag
     6961 cccagtcacc caagaacctg agctagcatc aggtggtggt ggtagtggtg gagttggcaa
     7021 aaaggagcag ctgtctgtga agaagcttcg agtagtactg tttgctctat gctgcaatac
     7081 agaacaggca gctgaacact tccgaaatcc ccagcgacgt attcgccgtt ggcttcgacg
     7141 tttccaggcc tcccaggggg agaatctaga gggcaaatat ctgagctttg aggcagaaga
     7201 gaaactggct gagtgggtgc taacccagcg cgaacaacag ctacctgtaa atgaggagac
     7261 cttgttccag aaggccacca aaataggacg ttctttggaa ggggggttta agatctccta
     7321 tgagtgggct gtgcgtttca tgctgcggca ccacctgact ccccatgccc ggcgagctgt
     7381 ggcccacacc ctacctaagg atgtagcaga gaatgcagga ctcttcattg attttgtaca
     7441 acggcagatt cacaaccagg acttaccctt gtctatgatt gtggctattg atgagatctc
     7501 tttgttcctg gatacagagg tgctgagcag tgatgatcga aaggagaatg ccctgcagac
     7561 agtgggcaca ggggaacctt ggtgtgatgt agtcctagcc attctggcag atggcactgt
     7621 ccttcccacc ctggttttct acagagggca gatggatcag cctgctaaca tgccagactc
     7681 catattgcta gaggcaaagg agagtggcta cagtgatgac gagatcatgg agctgtggtc
     7741 aactcgagtg tggcagaagc acacagcttg ccagcgcagc aaaggcatgc ttgtgatgga
     7801 ctgtcatcgc actcacttgt cagaagaggt actggctatg cttagtgcct ctagcacttt
     7861 gcctgcagtg gtcccagcag gctgtagctc caaaattcag ccattagatg tatgcatcaa
     7921 aagaactgtc aagaacttcc tgcataaaaa atggaaggaa caggctcggg aaatggcaga
     7981 tactgcatgt gattctgatg tcctgcttca gctggtgctt gtctggctgg gtgaagtgct
     8041 aggtgtcatt ggggactgtc cagagctagt tcagcgctcc ttcctggtgg ctagtgttct
     8101 gcctggcccc gatggcaaca ttaactcacc tacaagaaat gctgacatgc aggaggagct
     8161 aattgcctcc ctagaggagc aactgaagct gagtggggaa cattctgagt cttccactcc
     8221 acgacccaga tcatctcctg aagagacaat tgagcctgaa agtcttcacc agctctttga
     8281 gggtgaaagt gagaccgagt ctttctatgg ctttgaagaa gctgacctag atctgatgga
     8341 gatttagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     8401 ttgtggaaag gacgagttgc caatattgat gatcgcttta cgcgttaagt cgacaatcaa
     8461 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     8521 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     8581 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     8641 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     8701 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     8761 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     8821 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     8881 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8941 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9001 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     9061 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     9121 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     9181 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     9241 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     9301 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     9361 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     9421 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     9481 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     9541 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     9601 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     9661 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     9721 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     9781 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9841 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9901 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9961 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10021 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10081 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10141 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10201 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10261 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10321 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    10381 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
    10441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
    10501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
    10561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
    10621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
    10681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
    10741 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
    10801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    11041 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    11101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    11161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    11221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    11281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    11341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    11401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    11461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    11521 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    11581 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    11641 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    11701 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    11761 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    11821 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11881 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11941 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    12001 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    12061 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    12121 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    12181 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    12241 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    12301 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    12361 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    12421 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    12481 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    12541 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
