LOCUS       sequence-413889-        2955 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 promoter part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-P_luxR-PLuxB
FEATURES             Location/Qualifiers
     source          1..2955
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(183..277)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(298..957)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     872..891
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1119..1137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1152..1191)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      2115..2172
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2196..2213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2367..2955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2447..2466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
      181 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      241 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      301 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      361 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      421 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      481 gtttaaatca aaactggtga aactcaccca gggattggct gacacgaaaa acatattctc
      541 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      601 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      661 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      721 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      781 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      841 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      901 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      961 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1021 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     1081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     1141 ggtctcacta ttccaaaaaa aaaggctcca aaaggagcct ttaattgtat ctcaattttt
     1201 aaagtatggg caatcaattg ctcctgttaa aattgcttta gaaatacttt ggcagcggtt
     1261 tgttgtattg agtttcattt gcgcattggt taaatggaaa gtgacagtac gctcactgca
     1321 acctaatatt tttgaaatat cccaagagct ttttccttcg catgcccacg ctaaacattc
     1381 tttttctctt ttggttaaat cgttgtttga tttattattt gctatattta tttttcgata
     1441 attatcaact agagaaggaa caattaatgg tatgttcata cacgcatgta aaaataaact
     1501 atctatatag ttgtcttttt ctgaatgtgc aaaactaagc attccgaagc cattgttagc
     1561 cgtatgaata gggaaactaa acccagtgat aagacctgat gttttcgctt ctttaattac
     1621 atttggagat tttttattta cagcattgtt ttcaaatata ttccaattaa ttggtgaatg
     1681 attggagtta gaataatcta ctataggatc atattttatc aaattagcgt catcataata
     1741 ttgcctccat tttttagggt aattatctag gattgaaata tcagatttaa ccatagaatg
     1801 aggataaatg atcgcgagta aataatattc acaatgtacc attttagtca tatcagataa
     1861 gcattgatta atatcattat tgcttctaca agctttaatt ttattaatta ttctgtatgt
     1921 gtcgtcggca tttatgtttt tcatattcac caccctgaat tgactctctt ccgggcgcta
     1981 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggcg cgccgcacct gtaggatcgt
     2041 acaggtttac gcaagaaaat ggtttgttac agtcgaataa agtacagaga cccatgccgt
     2101 ctcattagct gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta
     2161 aaaccgaaaa gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg
     2221 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2281 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2341 cgtaaaaagg ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac
     2401 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2461 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2521 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2581 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2641 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2701 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2761 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2821 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2881 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2941 aaaaaaggat ctcaa
//
