LOCUS       sequence-413890-        2794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 promoter part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-P_phlF-PPhlF
FEATURES             Location/Qualifiers
     source          1..2794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(183..277)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(298..957)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     872..891
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1119..1137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1152..1191)
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      1954..2011
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2035..2052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2206..2794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2286..2305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
      181 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      241 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      301 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      361 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      421 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      481 gtttaaatca aaactggtga aactcaccca gggattggct gacacgaaaa acatattctc
      541 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      601 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      661 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      721 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      781 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      841 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      901 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      961 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1021 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     1081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     1141 ggtctcacta ttccaaaaaa aaaggctcca aaaggagcct ttaattgtat ctcaacactg
     1201 tgtacccgga caaacaccat taatcagcag gaaggtaaat tcttcaatat cctgttcaac
     1261 ggtcaactgt tcggtcagca ggcgatacca acaaaaacca aaaatcatat ccagcagcag
     1321 ttcacgattg atatctttcg gcagttcacc attgctaatg gcatcttcaa ccagtttttt
     1381 cggtatctca cgacgacgtt ccataaactg atctttcagt tgggtcaggg ttacagggtc
     1441 caactgtgct tctgcaataa cacaacgaaa tgcttcacca caaatggttt cacgccaaac
     1501 tttccacaga ttatgcagca gaaaatccag atcggcttta aagctaccca aatccggaaa
     1561 tttacgtacc tgttcgattt cattttcata cacttcggca atcagtgctg ctttgttggt
     1621 ccaccaacga taaatggtcg gtttgcctgc accggcgcga cgtgccacgc tttcaatgct
     1681 cagaccgcta taaccacatt ctttcaggat ttcaatggtg ctggtcagaa ttgctttatg
     1741 ggtatgcgga ctacgcaggc taccaatgct gctacggctc ggggtacgtg ccatattcac
     1801 ccccatgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcacca
     1861 ttcgatggcg cgccgccgac gtacggtgga atctgattcg ttaccaattg acatgatacg
     1921 aaacgtaccg tatcgttaag gtgtacagag acctccggca aaaaagggca aggtgtcacc
     1981 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     2041 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2101 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2161 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg
     2221 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2281 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     2341 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     2401 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     2461 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     2521 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     2581 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     2641 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2701 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2761 gcagcagatt acgcgcagaa aaaaaggatc tcaa
//
