LOCUS       sequence-413909-        2607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 CDS part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-C_NanoLuc
FEATURES             Location/Qualifiers
     source          1..2607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(183..277)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(298..957)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     872..891
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1119..1137
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1141..1184)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1187..1208
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      1767..1824
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1848..1865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2019..2607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2099..2118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ctcgaggctt
      181 ggattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
      241 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      301 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      361 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      421 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      481 gtttaaatca aaactggtga aactcaccca gggattggct gacacgaaaa acatattctc
      541 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      601 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      661 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      721 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      781 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      841 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      901 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      961 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1021 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aactcgagtg ccacctgacg
     1081 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga
     1141 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc
     1201 ttctagaggt ctcacatatg gtttttactc tggaagattt tgttggcgat tggcgtcaga
     1261 ccgcgggtta taatttggat caagtcctgg aacagggtgg cgtaagctct ctgttccaga
     1321 acctgggtgt gagcgtgacg ccgattcagc gcatcgttct gtccggcgag aacggtctga
     1381 aaattgatat tcatgtgatc atcccgtacg aaggcctgag cggtgaccaa atgggtcaaa
     1441 tcgagaaaat ctttaaagtc gtctacccag ttgacgatca ccacttcaag gttatcttgc
     1501 attacggtac gctggtgatt gatggtgtga ccccgaatat gattgactat ttcggccgtc
     1561 cgtatgaagg cattgccgtt tttgacggta aaaagatcac cgtcaccggt accctgtgga
     1621 atggcaataa gattattgac gagcgtctga ttaacccgga cggcagcctg ctgttccgcg
     1681 tgaccatcaa cggtgtcacg ggttggcgtc tgtgcgagcg catcctggca taatcgaaga
     1741 gacccatgcc gtctcattag ctgcagtccg gcaaaaaagg gcaaggtgtc accaccctgc
     1801 cctttttctt taaaaccgaa aagattactt cgcgttatgc aggcttcctc gctcactgac
     1861 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     1921 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1981 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct ccgcccccct
     2041 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2101 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2161 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2221 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2281 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2341 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2401 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2461 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2521 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2581 attacgcgca gaaaaaaagg atctcaa
//
