LOCUS       sequence-413910-        2141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Type 0 terminator part .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Shapiro M
  TITLE     EcoFlex MoClo Extension, Volume I
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: pBP-T_dummy-term
FEATURES             Location/Qualifiers
     source          1..2141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(161..255)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(276..935)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     850..869
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1097..1115
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1119..1162)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1165..1184
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      1279..1336
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1360..1377)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1531..2119)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1611..1630)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
       61 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
      121 gtatatatga gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg
      181 cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat
      241 ctatcaacag gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg
      301 cagtactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga
      361 tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg
      421 gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa
      481 ctcacccagg gattggctga cacgaaaaac atattctcaa taaacccttt agggaaatag
      541 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
      601 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
      661 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
      721 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
      781 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag
      841 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
      901 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
      961 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
     1021 cctcttacgt gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat
     1081 gacattaacc tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt
     1141 agctttcgct aaggatgatt tctggaattc gcggccgctt ctagaatcta gagacgcata
     1201 tgggtctcat cgaggcctgt ggagttgcat ggtaacactc ccagtgttag agaccgcatg
     1261 ccgtctcatt agctgcagtc cggcaaaaaa gggcaaggtg tcaccaccct gccctttttc
     1321 tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc tcgctcactg actcgctgcg
     1381 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1441 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1501 gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg ctccgccccc ctgacgagca
     1561 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1621 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1681 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1741 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1801 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1861 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1921 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1981 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2041 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2101 cagaaaaaaa ggatctcaag aagatccttt gatcttttct a
//
