LOCUS       sequence-41395-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS
  TITLE     An improved reverse genetics system for mammalian orthoreoviruses.
  JOURNAL   Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29.
  PUBMED    20042210
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2010-03-15"; volume: "398(2)"; pages: "194-200. Epub 2009 Dec
            29"
COMMENT     Sequence Label: pT7-M2-S2-S3-S4T3D
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(66..113)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     109..127
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gccggccacg atgcgtccgg cgtagaggat ctggctagcg atccggatat agttcctcct
       61 ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tagttattgc
      121 tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc cggatccttt
      181 tttttgagct ctcccttagc catccgagtg gacgacgtcc tccttcggat gcccaggtcg
      241 gaccgcgagg aggtggagat gccatgccga cccgatgatt tgcctgcatc ccttaacccc
      301 gcgtcaccag ggcgagcggc ctaacgtgtg tacccacgtt tgaccactcc tgcgtatgag
      361 tcgcgcttca ctcggcgatt agacgattca ccactatctc cgctagtcgg acaatcgggc
      421 tttggtgctg gaaaatgcgc tgacacttta tccaagaatc cttgagtcca attctggata
      481 gactcagatg agctcagctt actcactgca gtcttcactc ccgctgatag acttttagta
      541 cgcagactag ttttcactcc ctgtgccaat gcaatccaat tattcttgtc tgataacact
      601 ggcgccgcct gcgtgatgat tgtgctcggg tcaccaatga aagcctgcat tgccctagat
      661 gcaattttag aaaggat
//
