LOCUS       sequence-413964-         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For N-terminal tagging with mStayGold. c4 is the appendage that 
            facilitates fusions to the C-terminus of mStayGold..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano
            M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, 
            Miyawaki A
  TITLE     StayGold variants for molecular fusion and membrane-targeting 
            applications.
  JOURNAL   Nat Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6.
  PUBMED    38036853
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2023 Nov 30. doi: 10.1038/s41592-023-02085-6."
COMMENT     Sequence Label: pRSETB/mStayGold(c4)
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..678
                     /codon_start=1
                     /product="bright and highly photostable monomeric green 
                     fluorescent protein (Ando et al., 2023)"
                     /label=mStayGold(J)
                     /note="includes multiple mutations plus an N-terminal 
                     iinker sequence"
                     /translation="MVSTGEELFTGVVPFKFQLKGTINGKSFTVEGEGEGNSHEGSHKG
                     KYVCTSGKLPMSWAALGTSFGYGMKYYTKYPSGLKNWFHEVMPEGFTYDRHIQYKGDGS
                     IHAKHQHFMKNGTYHNIVEFTGQDFKENSPVLTGDMDVSLPNEVQHIPIDDGVECTVTL
                     QYPLLSDESKCVEAYQNTIIKPLHNQPAPDVPFHWIRKQYTQSKDDTEERDHIIQSETL
                     EAHL"
ORIGIN
        1 atggtgtcta caggcgagga gctgtttacc ggcgtggtgc ccttcaagtt ccagctgaag
       61 ggcaccatca acggcaagag cttcaccgtg gaaggcgagg gcgagggcaa tagccacgag
      121 ggcagccaca aaggcaagta cgtgtgcacc agcggcaaac tgccaatgtc ttgggccgcc
      181 ctgggaacta gcttcggcta tggcatgaag tactacacca agtaccccag cggcctgaag
      241 aactggttcc acgaggtgat gcccgagggc ttcacctacg acagacacat ccagtacaag
      301 ggcgacggca gcatccacgc caagcaccag cacttcatga agaacggcac ctaccacaac
      361 atcgtggagt tcaccggcca ggacttcaag gagaacagcc ccgtgctgac cggcgacatg
      421 gacgtgagcc tgcccaacga ggtgcagcac atccccatag atgacggcgt ggagtgcaca
      481 gtgaccctgc agtaccctct gctgagcgac gagagcaagt gcgtggaggc ctaccagaac
      541 accatcatca agcccctgca caatcagcca gcccccgatg tgccattcca ctggatcaga
      601 aagcagtaca cccagagcaa ggacgacacc gaggagagag accacatcat ccagagcgag
      661 accctggagg cccacctgcc atggcacgag ccttctgctt ctgccgtgta a
//
