LOCUS       sequence-413978-       23697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning in complete guide expression cassettes, with Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23697)
  AUTHORS   Lawrenson T, Clarke M, Kirby R, Forner M, Steuernagel B, Harwood W
  TITLE     An Optimised CRISPR Cas9 and Cas12a Toolkit for Barley and Wheat
  JOURNAL   Research Square, 12 March 2024, PREPRINT
REFERENCE   2  (bases 1 to 23697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Research 
            Square, 12 March 2024, PREPRINT"
COMMENT     Sequence Label: EC64434
FEATURES             Location/Qualifiers
     source          1..23697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    32..56
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             365..994
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1423..2496
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2562..2756
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        2818..2922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2923..3717
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSLREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3888..4476
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4377..4396
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4612..4636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    4724..4898
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     intron          5620..5809
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(6239..6584)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6276..6296)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      complement(7600..7852)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7868..7888)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13621..13641)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(15672..15924)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(15940..15987)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     RBS             22597..22605
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    22718..22739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(22754..22759,22760..22777,22778..22784)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    22792..22808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             22826..22837
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             22844..23521
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      23566..23637
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      23653..23680
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 cacgaagtga tccgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
       61 gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg
      121 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggagtca gccgtgcggc
      181 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
      241 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
      301 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
      361 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
      421 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
      481 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
      541 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
      601 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
      661 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt
      721 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
      781 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
      841 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
      901 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
      961 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
     1021 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
     1081 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
     1141 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
     1201 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
     1261 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
     1321 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
     1381 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgagcgg tcgcaaacca
     1441 tccggcccgg tacaaatcgg cgcggcgctg ggtgatgacc tggtggagaa gttgaaggcc
     1501 gcgcaggccg cccagcggca acgcatcgag gcagaagcac gccccggtga atcgtggcaa
     1561 gcggccgctg atcgaatccg caaagaatcc cggcaaccgc cggcagccgg tgcgtcgtcg
     1621 attaggaagc cgcccaaggg cgacgagcaa ccagattttt tcgttccgat gctctatgac
     1681 gtgggcaccc gcgatagtcg cagcatcatg gacgtggccg ttttccgtct gtcgaagcgt
     1741 gaccgacgag ctggcgaggt gatccgctac gagcttccag acgggcacgt agaggtttcc
     1801 gcagggccgg ccggcatggc cagtgtgtgg gattacgacc tggtactgat ggcggtttcc
     1861 catctaaccg aatccatgaa ccgataccgg gaagggaagg gagacaagcc cggccgcgtg
     1921 ttccgtccac acgttgcgga cgtactcaag ttctgccggc gagccgatgg cggaaagcag
     1981 aaagacgacc tggtagaaac ctgcattcgg ttaaacacca cgcacgttgc catgcagcgt
     2041 acgaagaagg ccaagaacgg ccgcctggtg acggtatccg agggtgaagc cttgattagc
     2101 cgctacaaga tcgtaaagag cgaaaccggg cggccggagt acatcgagat cgagctagct
     2161 gattggatgt accgcgagat tacagaaggc aagaacccgg acgtgctgac ggttcacccc
     2221 gattactttt tgatcgatcc cggcatcggc cgttttctct accgcctggc acgccgcgcc
     2281 gcaggcaagg cagaagccag atggttgttc aagacgatct acgaacgcag tggcagcgcc
     2341 ggagagttca agaagttctg tttcaccgtg cgcaagctga tcgggtcaaa tgacctgccg
     2401 gagtacgatt tgaaggagga ggcggggcag gctggcccga tcctagtcat gcgctaccgc
     2461 aacctgatcg agggcgaagc atccgccggt tcctaatgta cggagcagat gctagggcaa
     2521 attgccctag caggggaaaa aggtcgaaaa agcttctttc ctgtggatag cacgtacatt
     2581 gggaacccaa agccgtacat tgggaaccgg aacccgtaca ttgggaaccc aaagccgtac
     2641 attgggaacc ggtcacacat gtaagtgact gatataaaag agaaaaaagg cgatttttcc
     2701 gcctaaaact ctttaaaact tattaaaact cttaaaaccc gcctggcctg tgcataactg
     2761 tctggccagc gcacagccga acagctgcaa aaagcgccca catcaaggct ccgagtgcgc
     2821 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     2881 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatggctaa aatgagaata
     2941 tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg taaaagatac ggaaggaatg
     3001 tctcctgcta aggtatataa gctggtggga gaaaatgaaa acctatattt aaaaatgacg
     3061 gacagccggt ataaagggac cacctatgat gtggaacggg aaaaggacat gatgctatgg
     3121 ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg aacggcatga tggctggagc
     3181 aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg aagagtatga agatgaacaa
     3241 agccctgaaa agattatcga gctgtatgcg gagtgcatca ggctctttca ctccatcgac
     3301 atatcggatt gtccctatac gaatagctta gacagccgct tagccgaatt ggattactta
     3361 ctgaataacg atctggccga tgtggattgc gaaaactggg aagaggacac tccatttaaa
     3421 gatccgcgcg agctgtatga ttttttaaag acggaaaagc ccgaagagga acttgtcttt
     3481 tcccacggcg acctgggaga cagcaacatc tttgtgaaag atggcaaagt aagtggcttt
     3541 attgatcttg ggagaagcgg cagggcggac aagtggtatg acattgcctt ctgcgtccgg
     3601 tcgctcaggg aggatatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
     3661 atcaagcctg attgggagaa aataaaatat tatattttac tggatgaatt gttttagctg
     3721 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     3781 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     3841 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     3901 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     3961 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4021 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4081 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4141 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4201 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4261 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4321 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4381 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4441 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     4501 cggttcctgc tcggatctgt tggaccggac agtagtcatg gttgatgggc tgcctgtatc
     4561 gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg gtggcaggat
     4621 atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc ggacgttttt
     4681 aatgtactgg ggttgaacac tctgtgccga attcggatcc agcgatctgg attttagtac
     4741 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
     4801 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
     4861 ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc gatcgactct
     4921 agctagagaa gcctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc
     4981 ggcgagtact tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt
     5041 acgcccgaca gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc
     5101 tgcatcatcg aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag
     5161 catatacgcc cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg
     5221 cgtctgctgc tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta
     5281 ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc
     5341 cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc
     5401 ctcggcccaa agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc
     5461 catcacagtt tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca
     5521 tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag
     5581 atcggccgca gcgatcgcat ccatggcctc cgcgaccggc tgcagttatc atcatcatca
     5641 tagacacacg aaataaagta atcagattat cagttaaagc tatgtaatat ttacaccata
     5701 accaatcaat taaaaaatag atcagtttaa agaaagatca aagctcaaaa aaataaaaag
     5761 agaaaagggt cctaaccaag aaaatgaagg agaaaaacta gaaatttacc tgcagaacag
     5821 cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc
     5881 aataggtcag gctctcgctg aattccccaa tgtcaagcac ttccggaatc gggagcgcgg
     5941 ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg cagctattta
     6001 cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat tcttcgccct
     6061 ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac ttctcgacag
     6121 acgtcgcggt gagttcaggc tttttcattg tatcgataat tgtaaatgta attgtaatgt
     6181 tgtttgttgt ttgttgttgt tggtaattgt tgtaaaaatg agctcttata ctcgagcgtg
     6241 tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg
     6301 attgtgcgtc atcccttacg tcagtggaga tgtcacatca atccacttgc tttgtagacg
     6361 tggttggaac ctcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     6421 cactgtcggc agagagatct tgaatgatag cctttccttt atcgcaatga tggcatttgt
     6481 aggagccacc ttccttttct actgtccttt cgatgaagtg acagatagct gggcaatgga
     6541 atccgaggag gtttcccgaa attatccttt gttgaaaagt ctcaatagcc ctttgatctt
     6601 ctgagactgt atctttgaca tttttggagt agaccagagt gtcgtgctcc accatgttga
     6661 cgaagatttt cttcttgtca ttgagtcgta aaagactctg tatgaactgt tcgccagtct
     6721 tgacggcgag ttctgttaga tcctcgattt gaatcttaga ctccatgcat ggccttagat
     6781 tcagtaggaa ctaccttttt agagactcca atctctatta cttgccttgg tttatgaagc
     6841 aagccttgaa tcgtccatac tggaatagta cttctgatct tgagaaatat gtctttctct
     6901 gtgttcttga tgcaattagt cctgaatctt ttgactgcat ctttaacctt cttgggaagg
     6961 tatttgatct cctggagatt gttactcggg tagatcgtct tgatgagact tgctgcgtag
     7021 gcctctctaa ccatctgtgg gtcagcattc tttctgaaat tgaagaggct aaccttctca
     7081 ttatcagtgg tgaacatagt gtcgtcacct tcaccttcga acttccttcc tagatcgtaa
     7141 agatagagga aatcgtccat tgtaatctcc ggggcaaagg agatctcttt tggggctgga
     7201 tcactgctgg gccttttggt tcctagcgtg agccagtggg ctttttgctt tggtgggctt
     7261 gttagggcct tagcaaagct cttgggcttg agttgagctt ctcctttggg gatgaagttc
     7321 aacctgtctg tttgctgact tgttgtgtac gcgtcagctg ctgctcttgc ctctgtaata
     7381 gtggcaaatt tcttgtgtgc aactccggga acgccgtttg ttgccgcctt tgtacaaccc
     7441 cagtcatcgt atataccggc atgtggaccg ttatacacaa cgtagtagtt gatatgaggg
     7501 tgttgaatac ccgattctgc tctgagagga gcaactgtgc tgttaagctc agatttttgt
     7561 gggattggaa ttcctccgca agaattcaag cttagcgtcg atctagtaac atagatgaca
     7621 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     7681 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     7741 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     7801 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tctgcttgac
     7861 aagctcagac tttacgcttc ttctttgggt cagccctaga gtcaccaccc aactgcgaca
     7921 gatctatcct tgtctcatag agccctgtaa tagattggtg aataagggta gcgtccaaca
     7981 cttccttggt tgatgtgtag cgctttctgt cgatagtggt atcgaagtac ttaaaggctg
     8041 ctggggctcc cagattggtc agtgtgaaca ggtggatgat gttttccgct tgttccctta
     8101 tcggcttatc tctgtgcttg ttgtacgcgg aaagcacctt taattgtaga aaaccagtaa
     8161 aacatggaca attaggaggc atcgattatc taaccaaaga ctttaacaac actaatctgc
     8221 cactaatatt catgagaagt ccttacctta tcgaggttag cgtctgcaag aatgacccgc
     8281 ttggaaaatt cgctgatctg ctctatgatc tcatcaagat agtgcttgtg ctgctcgaca
     8341 aacaactgct tctgctcgtt gtcctctggt gaccccttca gcttctcata gtgtgaggcc
     8401 aagtacagga agttgacgta cttactgggc aatgccagct cgttgccctt ctgcagttct
     8461 ccggcagatg ccagcatgcg ttttctgcca ttttccaatt cgaaaagcga atacttgggc
     8521 agtttgataa tgaggtcttt cttcacctct ttgtaacctg gtttgtataa ttttggttca
     8581 gttaattagg aaaattacat aatggctcca aaaaaaaatt atcgaccttt taccaaaaaa
     8641 aataaaattt atcgaccttg acaggaaaaa gttacaaatc aaaagaaaga atatcgtctg
     8701 gcatccaata ttttaccttt agcctcgagg aagtcgatcg gattcttctc gaacgaggac
     8761 ctttccatta tggtaattcc aagcagttcc ttgacgcttt tcagtttctt gctcttgccc
     8821 ttctccacct tggccactac cagtacgctg tatgccacgg tagggctatc aaagcctccg
     8881 tatttcttcg gatcccaatc cttcttcctc gcaatcagtt tgtcggagtt gcgcttaggg
     8941 agaatggact ccttggagaa accaccggtt tgaacctccg ttttcttgac tatgttgact
     9001 tggggcatgc tgagaacctt acgcacagtc gcaaaatccc tgcccttatc ccagacaatc
     9061 tcacctgcaa gaaacagcaa agagagagtt aagatataag tgaacaagaa agtcttggga
     9121 tcgacataca tacaatcaat tgatcaaatt cctaggatta aacatatgaa ataggaaaga
     9181 cagacctgtt tcgccatttg tctcgatcag aggcctcttc cggatttcac cattcgccag
     9241 agtgatctct gtcttgaaga agttcatgat attcgagtaa aagaagtatt tcgcggtagc
     9301 cttgccgatc tcttgttctg acttcgcgat catcttccta acgtcgtaca ccttgtaatc
     9361 cccgtacaca aactcagact ccagtttcgg atacttcttg atgagagcag tgccaacaac
     9421 ggcgttcagg taggcgtcat gggcatgatg gtagttgttg atctcgcgaa ccttgtaaaa
     9481 ctggaagtcc ttccggaagt cactaaccag tttgctcttg agggtgatca cctgcgtaac
     9541 catcaaaagt caatatggat tcatcacaaa ttaatcagga atagaaagat cgggtggcct
     9601 tgaagttgat caaataaaaa gtttcgaact ttagtttgat atggtaaagg aaagttggaa
     9661 actttacctt tacctccctg ataagtttgt cattctcatc atacttcgta ttcatacgcg
     9721 aatcgagtat ctgggccaca tgtttcgtaa tctgacgagt ttcaaccaac tggcgcttga
     9781 tgaagccggc tttgtcgagc tcagacagtc cgcccctttc tgccttcgtg agattgtcga
     9841 atttccgttg ggttatgagc ttagcattca aaagctgcct ccaatagttc ttcatcttct
     9901 tcacaacctc ctcgctgggt acattgtcgg attttccgcg gttcttgtct gagcgcgtaa
     9961 ggacctataa cacgggagaa accaaaaagc aaactcattt cacgccaaat actacagatt
    10021 tctcaaaaat taagttataa tccatatatc ctgacaagaa attgattaaa acacagttgc
    10081 tttaccttgt tgtcaatact atcgtccttc aggaaagatt gagggacaat gtgatccaca
    10141 tcatagtcgg agagtctgtt aatgtcgagc tcttggtcga cgtacatatc tctcccattc
    10201 tggaggtaat agaggtacag cttctcgttc tggagttgag tattttcgac tgggtgctct
    10261 ttcagaatct gactcccaag ctccttgatg ccttcctcta tccgcttcat gcgctcccgt
    10321 gagttcttct gccctttctg agtcgtctgg ttttccctcg ccatctcgat gacgatgttc
    10381 tccggcttat ggcggcccat gacctgtagc aataattaaa acaaaaatac cacaaacagt
    10441 tagaactgag gcaagaagaa tgaatgcaga gaaaaaaaat cttagctatt catggaaatg
    10501 gaagtgcgac gaacacctaa aatgcaagga gcataaccaa atgcagaact taccttcacc
    10561 agctcatcca caaccttcac tgtctgcagt atgcccttct ttatagctgg cgatccagcc
    10621 aggttggcta tgtgttcgtg aaggctatcg ccctgacctg acacttgggc cttctggata
    10681 tcttctttga aggtcagtga gtcatcgtga ataagctgca tgaagttccg attcgcaaaa
    10741 ccgtcggatt tgagaaagtc cagtatagtc ttgccggatt gcttgtcgcg gatgccgttg
    10801 atgagcttcc tggagagcct tccccaacct aaaacataga acaaacattt gtctcatcac
    10861 aaatatgcat caaggaacac tcagaagtta gtcatatacc caaattcttg aaactagaga
    10921 aacaaaaaaa gagggatcat tgcggaaacc ttttgacctc ttacctgtat atctcctcct
    10981 cttcaactgc ttcatgacct tgtcatcgaa cagatgcgca taagtcttaa ggcgctcctc
    11041 gatcatttct ctgtcctcaa acagcgtgag agtcagcacg atgtcctcga ggatgtcctc
    11101 gttctcctcg ttgtcaagga agtccttgtc cttgatgatt ttcaacaggt catggtatgt
    11161 gccaagagag gcattgaacc tatcctcaac gccgctgatt tccacagaat caaagcattc
    11221 gatcttctta aagtagtcct ctttaagctg tttcacagtg accttccgat tggttttgaa
    11281 caagagatcg acaatggcct tcttctgctc acctgttata acagtaaaaa ttactttaga
    11341 aacaaaacaa aagtgtggtc ttttaggaat gattattgat cagttcatag tttaaaatct
    11401 tgtgttttta tcgtaaaagt gtggaccagg atttacctga caggaaggcc ggtttgcgca
    11461 tcccctccgt cacatatttc accttcgtca gctcgttgta gaccgtgaag tattcgtaca
    11521 ggagactatg cttgggcagg actttctcat ttgggagatt cttatcgaag ttggtcatcc
    11581 tttcgatgaa ggactgtgca ctcgcaccct tgtccacgac ctcttcgaag ttccaaggcg
    11641 tgatagtctc ctcggatttg cgtgtcatcc acgcaaaccg cgagttcccg cgtgcgagtg
    11701 gacctaatga attaatagaa aaatcaggca aattagaaaa gaaataattc tcaaaagaga
    11761 acaactgagg tcagaaaata gcattcatca actgaaacta ggtatttctt catatgatac
    11821 taacctacat agtagggaat gcggaacgtg aggatctttt caatcttctc tctgttgtct
    11881 ttcaggaacg gatagaagtc ctcttgcctc ctcaagatag catgcagctc accgaggtgg
    11941 atctggtgcg gtatgctgcc attgtcgaag gtacgctgct tccgaagcaa atcttcgcgg
    12001 ttcaatttaa cgagcagctc ctctgtaccg tccatctttt ccagaatagg cttgatgaat
    12061 ttgtagaatt cctcctgact tgcacctaat aatatcatca gaggaaaact caattcttat
    12121 atcttacaga aatgaacaac aaaatacatg tagcagagag gaaaacaatc ataagtaaca
    12181 acttacctcc atcaatgtac cctgcgtagc cattcttcga ttggtcaaag aaaatctcct
    12241 tgtacttctc ggggagttgc tggcgaacca aagccttcag aagggtcaga tcctggtgat
    12301 gctcgtcgta cctcttgatc atggaggcgc taagtggcgc tttggttatc tccgtgttga
    12361 cccgcagaat gtcactgagc aagatagcgt ctgacagatt cttggcagcc aagaacaggt
    12421 cggcatactg atcacctaca aaagtttaaa agactcaatt ttaactctac agcttcaaat
    12481 aaatttccgc aaagaaattg ataggtttag acaacaaaag aagagttcca atttcaagag
    12541 cacctatttg ggcgagcaga ttgtccagat catcatcgta tgtatccttt gacagctgca
    12601 gcttcgcgtc ctcggcgagg tcaaagttgc ttttgaagtt aggggtgagg cccagagaca
    12661 gtgcaatcag attcccgaac aagccgttct tcttctcgcc tggcaattga gcgatcagat
    12721 tctcaagccg tctggacttc gaaagtctgg cagacagtat tgccttggcg tcgacacctg
    12781 agattgtttg caaagaaaaa tctcaaagca tgtacaatct ataaacttat gatggaaaaa
    12841 gaaaagaaag gtaactaagg aatgttacct gatgcattga tggggttctc ctcgaacagc
    12901 tgattgtagg tttggacgag ctgaatgaac agtttatcca catcggagtt atccgggttg
    12961 aggtcccctt cgatcaagaa atggccacga aactttatca tgtgcgcgag agccaggtat
    13021 atgagccgaa ggtctgcctt atcggttgag tccaccagct tcttgcgaag gtggtatata
    13081 gtcggatact tttcatgata ggctacttcg tcaacaatat tcccaaagat ggggtgacgc
    13141 tcgtgtttct tatcttcctc taccaggaag gattcctcga ggcgatggaa gaacgagtcg
    13201 tcaacctgca aaatcaagtt tattgatcat caagtgagtt tacatgtagt taaatgcttt
    13261 cagtaatatc ccaatgaagc aggatatgct ttcagtacaa aatacataag catactatat
    13321 cataaaaatc cttaccttcg ccatctcgtt gctaaagatc tcttggagat aacagattct
    13381 gttcttcctg cgagtgtacc ttctcctcgc agtacgttta agccttgtcg cctccgcggt
    13441 ttctccagag tcaaagagca gagctccgat caagttcttc ttaatgctgt gcctatctgt
    13501 gttgcccagc actttaaatt tcttcgaagg taccttgtat tcatcggtga taacggccca
    13561 cccaactgag ttcgtaccaa tatcaagtcc aatgctgtac ttcttgtcca ttttccagct
    13621 gaccttccgc ttcttcttcg gtgggctaga agccattcct gcagaagtaa caccaaacaa
    13681 cagggtgagc atcgacaaaa gaaacagtac caagcaaata aatagcgtat gaaggcaggg
    13741 ctaaaaaaat ccacatatag ctgctgcata tgccatcatc caagtatatc aagatcaaaa
    13801 taattataaa acatacttgt ttattataat agataggtac tcaaggttag agcatatgaa
    13861 tagatgctgc atatgccatc atgtatatgc atcagtaaaa cccacatcaa catgtatacc
    13921 tatcctagat cgatatttcc atccatctta aactcgtaac tatgaagatg tatgacacac
    13981 acatacagtt ccaaaattaa taaatacacc aggtagtttg aaacagtatt ctactccgat
    14041 ctagaacgaa tgaacgaccg cccaaccaca ccacatcatc acaaccaagc gaacaaaaag
    14101 catctctgta tatgcatcag taaaacccgc atcaacatgt atacctatcc tagatcgata
    14161 tttccatcca tcatcttcaa ttcgtaacta tgaatatgta tggcacacac atacagatcc
    14221 aaaattaata aatccaccag gtagtttgaa acagaattct actccgatct agaacgaccg
    14281 cccaaccaga ccacatcatc acaaccaaga caaaaaaaag catgaaaaga tgacccgaca
    14341 aacaagtgca cggcatatat tgaaataaag gaaaagggca aaccaaaccc tatgcaacga
    14401 aacaaaaaaa atcatgaaat cgatcccgtc tgcggaacgg ctagagccat cccaggattc
    14461 cccaaagaga aacactggca agttagcaat cagaacgtgt ctgacgtaca ggtcgcatcc
    14521 gtgtacgaac gctagcagca cggatctaac acaaacacgg atctaacaca aacatgaaca
    14581 gaagtagaac taccgggccc taaccatgga ccggaacgcc gatctagaga aggtagagag
    14641 gggggggggg ggaggacgag cggcgtacct tgaagcggag gtgccgacgg gtggatttgg
    14701 gggagatctg gttgtgtgtg tgtgcgctcc gaacaacacg aggttgggga aagagggtgt
    14761 ggagggggtg tctatttatt acggcgggcg aggaagggaa agcgaaggag cggtgggaaa
    14821 ggaatccccc gtagctgccg tgccgtgaga ggaggaggag gccgcctgcc gtgccggctc
    14881 acgtctgccg ctccgccacg caatttctgg atgccgacag cggagcaagt ccaacggtgg
    14941 agcggaactc tcgagagggg tccagaggca gcgacagaga tgccgtgccg tctgcttcgc
    15001 ttggcccgac gcgacgctgc tggttcgctg gttggtgtcc gttagactcg tcgacggcgt
    15061 ttaacaggct ggcattatct actcgaaaca agaaaaatgt ttccttagtt tttttaattt
    15121 cttaaagggt atttgtttaa tttttagtca ctttatttta ttctatttta tatctaaatt
    15181 attaaataaa aaaactaaaa tagagtttta gttttcttaa tttagaggct aaaatagaat
    15241 aaaatagatg tactaaaaaa attagtctat aaaaaccatt aaccctaaac cctaaatgga
    15301 tgtactaata aaatggatga agtattatat aggtgaagct atttgcaaaa aaaaaggaga
    15361 acacatgcac actaaaaaga taaaactgta gagtcctgtt gtcaaaatac tcaattgtcc
    15421 tttagaccat gtctaactgt tcatttatat gattctctaa aacactgata ttattgtagt
    15481 actatagatt atattattcg tagagtaaag tttaaatata tgtataaaga tagataaact
    15541 gcacttcaaa caagtgtgac aaaaaaaata tgtggtaatt ttttataact tagacatgca
    15601 atgctcatta tctctagaga ggggcacgac cgggtcacgc tgcacctcca ctagaattcg
    15661 agctcagcgt cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
    15721 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
    15781 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
    15841 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
    15901 ttattgccaa atgtttgaac gatctgcttg acaagctcat ttcttcttct tagcctgacc
    15961 agctttctta gtagcagcag gcctcttgtg cttaacggag gtctgagcgt actcaagcca
    16021 ctctttgttg gagatggcga tcttcacctt gtccagcttc tcatcttccg ctttcttaaa
    16081 ctgcccgata gcccagagaa cctataacac gggagaaacc aaaaagcaaa ctcatttcac
    16141 gccaaatact acagatttct caaaaattaa gttataatcc atatatcctg acaagaaatt
    16201 gattaaaaca cagttgcttt acctttctcg caatgttgta agccccgttt gcatcagcat
    16261 tcttcggcag gatagcgttc tcttgagcct catagttacg gctgtcgtag aagatcccgt
    16321 cagagttctt gaccggagag ataaggaagt ccacatcggt ccttccggtg atagagttcc
    16381 tcatctggag catgagagac atgagggcca tgaatgagct gtagaatgcc ttgtcgctct
    16441 gctcgcacaa aagagccctg atatcaccct gctggtagtt gatgccgtac ttgttgaaca
    16501 gttctttgta ggcagaggtg aggcacactt cttcccagtc gaacacgttg tttttcttcg
    16561 gatttcggaa gatcctgata cggttcccgt aggagtagag cttccacttc ttaatgtagt
    16621 cggcgtcggt cctgctgaag ttcttatagt cgagagcgaa ctcgaaaaga tcctcttccg
    16681 gcacgtacat gatcctgtcg aaagacgaga tgaacttctt gctgtcggcg atcgaggtgt
    16741 acttggtctt gagcaggttc acgaaaccag tagaaggatc gatcttgctg gtgagccaag
    16801 cagggatata gaagatgaag ccgttctggg tgctcatgct cttgaaggac tcgaacttgt
    16861 tcgtgatctg gtatccctta agagcaccac cggtagcgca agggttgctc tttttgtcca
    16921 ccatgtagtt gagcttgtcg atgagcatct tctcgaactt ttggtagacc tgcttctcaa
    16981 cctgtagcaa taattaaaac aaaaatacca caaacagtta gaactgaggc aagaagaatg
    17041 aatgcagaga aaaaaaatct tagctattca tggaaatgga agtgcgacga acacctaaaa
    17101 tgcaaggagc ataaccaaat gcagaactta ccttcactct actgttcttg aacccagagt
    17161 tgaggtcctc aagagcgatc acagcatcgt acttttccac cagctcgcag atcttatgca
    17221 ccacctgaga gatgtagccg gccttcaact ctttgatgtt ttcgatagaa gtccagttct
    17281 gcctggcctc gaaacgctct ttttctttct tatcgaggag tgagtggtag tcggtcttga
    17341 tcctgatgcc gttgaaattg ttgataatct cgttcaggct gtactgctcg acgatgtttc
    17401 cctttccgtc caccacaacg atgtagagga ggtttctctc accacgatcg attccgatca
    17461 cgtaagggtt atcatcgtgc ttgagcagca ccctcacctt taattgtaga aaaccagtaa
    17521 aacatggaca attaggaggc atcgattatc taaccaaaga ctttaacaac actaatctgc
    17581 cactaatatt catgagaagt ccttacctcg gtattgatct tgaagatgtt cttcgggcac
    17641 ttgttgatag cgatcgggat atgaagctcg tactggtcct cgctgaacct tttgtccttg
    17701 tagacgtcgt aagacagcgt ggtggtcttt ttcgggttgt caggattctt gttagcgatt
    17761 ggagagttgg ccgggtgaac aaccaactct tctttcttga gtgaggccct acgcatgaag
    17821 agttcagcac caccagaaag tctgatctgc ccgtggttgt tctcgtcgaa aagcagcttg
    17881 aagtacatcg tatggaggtt aggagttccg tggctcttgt cggagaagtc cttgttatag
    17941 atctggaaca tgtagagctt gccctcttcg accaacttgt caacctgtta taacagtaaa
    18001 aattacttta gaaacaaaac aaaagtgtgg tcttttagga atgattattg atcagttcat
    18061 agtttaaaat cttgtgtttt tatcgtaaaa gtgtggacca ggatttacct ctttcttgga
    18121 ggcgctctcg aagctaactt tgtaaccctg ttcctcaacc tcacggtaga acccggcgat
    18181 atccttgtac ttttctgtct cgctgaagtt aaagtcgtaa gcgttagacc acttcgggta
    18241 acgcgagatg ctgtccttga agaaatcgat gagcttgtgg cagtcgttga ggttgaacat
    18301 gtcgcctttc ttgaaggtcc cgttcttgta gattttctgg atgtcctcag acgggttgta
    18361 gtaggccatc catttcttgc tgaagaacac cttaggaagc atcttgttcg gtcccggaag
    18421 gagcttgtag ttgatcttct catagttccc gttcacgtca tccttgtcga tcttttggag
    18481 gcacttggcg tatttcttgt ccatgatggc caggtagtac ttagacccgt acctaatgaa
    18541 ttaatagaaa aatcaggcaa attagaaaag aaataattct caaaagagaa caactgaggt
    18601 cagaaaatag cattcatcaa ctgaaactag gtatttcttc atatgatact aacctgagga
    18661 tggtggccct gtaatctgtc tctttgtctt tgtcccatcc gcccatgaac tgcgggttct
    18721 gaaagtacaa cttgaacttg tccttgctgt aaggcttctg ggtcacgtag tttctgatgg
    18781 cgtcgtagat atggtccacc ttgaggagga tatcgtaagc gagcacgaaa tccccgtaga
    18841 aagactcgtc cctattagtc tctttgccct ctccgaagaa ggccttgata tagttctcga
    18901 aggacttcac gctgtcgagc aaatccttca tgatcgcgac gacagcgtcg ttcttcttca
    18961 gactcttctc gaggacgaaa tcagcatcga acaacttctc agaggacccg tacacctaat
    19021 aatatcatca gaggaaaact caattcttat atcttacaga aatgaacaac aaaatacatg
    19081 tagcagagag gaaaacaatc ataagtaaca acttaccttg tagatctcgt cgaccttctg
    19141 gatgatgatc tctttgagct tctcgaccac gctgagatca gcatcagcgt attcttgaag
    19201 ctgctcgagg ctgaaagagc cgatcttctt gaacgactta cgcctgtcat cctcgtactt
    19261 ctcagtgacg acagccttct tcttgaggtg gatgtcatcg tactcagcat tccacttgtc
    19321 cctgatcacg ttccactcgc cgaaaatatc cttgctgatg gtgctgatag caggtccgtt
    19381 cttaacgaag atcccagcgc tgctgtactc gtcgaagttc ttgaaaagct tctccaactt
    19441 cttgatggac gagaagatct cgctgttctt gttgagggtg ttacggaaaa cctcaagcac
    19501 ttcctcgtca gaggtgtaac cctcaccgta aaatgagagg ctctcacgat cagagagaac
    19561 ctgcaagaaa cagcaaagag agagttaaga tataagtgaa caagaaagtc ttgggatcga
    19621 catacataca atcaattgat caaattccta ggattaaaca tatgaaatag gaaagacaga
    19681 cctgcttgta gagcggcttg aacttcggga gcttctgctt cgttttctgg ttgtacaggt
    19741 tgatgtactc gttgagtccc ttaatcttct ccccagactc ggtcacgaat cctccgataa
    19801 tagcgttgta cacgtcgatg ccctcttggg tgagaacgaa gttgaagaac tccccttcaa
    19861 aaaagtcctc gacgtcgtag tcgctgttga ggatcttctc tttgatttct tgcacctcgt
    19921 gcttatcgaa gatggcgtcc acctgagatt gtttgcaaag aaaaatctca aagcatgtac
    19981 aatctataaa cttatgatgg aaaaagaaaa gaaaggtaac taaggaatgt taccttctcg
    20041 aagatgtcca tgttgctgat gtaacgcgtg aggttctcgt tgatgcatct gaaagcgata
    20101 gaggtgctct tggcctcttc gctgaacatg ttttccctgt ttctgaagaa tccggtgaag
    20161 gcagtagtga atccgttgaa gctgttcacg agagcgatct catccttatc gtccaggaac
    20221 tcaggcagga ttgtctcgat aatatccttc ttgaagaggc tcttgtatcc ctcgtttccc
    20281 ttgaaagcct tggcgatctc tttacggagg ttgatctcga ggttctcaag ctctttgttc
    20341 tctttctcgg tcctggtttt cttacggaag aggctgatgt agttgttgag gttcttgagc
    20401 ttgatgctgt ggagcacatc gttgatgaag ctgaggtagt acctgtcgag gagcttcttc
    20461 acgcccttgt aatcttcagc tctcttctca tcctcgacga ggagcctctt gttgtcgata
    20521 ttctcttggg tctttcccac agggatagcc ttgaacctga gggtcttaga gaggctgtag
    20581 cagttggtaa acttctcgag cttgctcatt cctgcagaag taacaccaaa caacagggtg
    20641 agcatcgaca aaagaaacag taccaagcaa ataaatagcg tatgaaggca gggctaaaaa
    20701 aatccacata tagctgctgc atatgccatc atccaagtat atcaagatca aaataattat
    20761 aaaacatact tgtttattat aatagatagg tactcaaggt tagagcatat gaatagatgc
    20821 tgcatatgcc atcatgtata tgcatcagta aaacccacat caacatgtat acctatccta
    20881 gatcgatatt tccatccatc ttaaactcgt aactatgaag atgtatgaca cacacataca
    20941 gttccaaaat taataaatac accaggtagt ttgaaacagt attctactcc gatctagaac
    21001 gaatgaacga ccgcccaacc acaccacatc atcacaacca agcgaacaaa aagcatctct
    21061 gtatatgcat cagtaaaacc cgcatcaaca tgtataccta tcctagatcg atatttccat
    21121 ccatcatctt caattcgtaa ctatgaatat gtatggcaca cacatacaga tccaaaatta
    21181 ataaatccac caggtagttt gaaacagaat tctactccga tctagaacga ccgcccaacc
    21241 agaccacatc atcacaacca agacaaaaaa aagcatgaaa agatgacccg acaaacaagt
    21301 gcacggcata tattgaaata aaggaaaagg gcaaaccaaa ccctatgcaa cgaaacaaaa
    21361 aaaatcatga aatcgatccc gtctgcggaa cggctagagc catcccagga ttccccaaag
    21421 agaaacactg gcaagttagc aatcagaacg tgtctgacgt acaggtcgca tccgtgtacg
    21481 aacgctagca gcacggatct aacacaaaca cggatctaac acaaacatga acagaagtag
    21541 aactaccggg ccctaaccat ggaccggaac gccgatctag agaaggtaga gagggggggg
    21601 gggggaggac gagcggcgta ccttgaagcg gaggtgccga cgggtggatt tgggggagat
    21661 ctggttgtgt gtgtgtgcgc tccgaacaac acgaggttgg ggaaagaggg tgtggagggg
    21721 gtgtctattt attacggcgg gcgaggaagg gaaagcgaag gagcggtggg aaaggaatcc
    21781 cccgtagctg ccgtgccgtg agaggaggag gaggccgcct gccgtgccgg ctcacgtctg
    21841 ccgctccgcc acgcaatttc tggatgccga cagcggagca agtccaacgg tggagcggaa
    21901 ctctcgagag gggtccagag gcagcgacag agatgccgtg ccgtctgctt cgcttggccc
    21961 gacgcgacgc tgctggttcg ctggttggtg tccgttagac tcgtcgacgg cgtttaacag
    22021 gctggcatta tctactcgaa acaagaaaaa tgtttcctta gtttttttaa tttcttaaag
    22081 ggtatttgtt taatttttag tcactttatt ttattctatt ttatatctaa attattaaat
    22141 aaaaaaacta aaatagagtt ttagttttct taatttagag gctaaaatag aataaaatag
    22201 atgtactaaa aaaattagtc tataaaaacc attaacccta aaccctaaat ggatgtacta
    22261 ataaaatgga tgaagtatta tataggtgaa gctatttgca aaaaaaaagg agaacacatg
    22321 cacactaaaa agataaaact gtagagtcct gttgtcaaaa tactcaattg tcctttagac
    22381 catgtctaac tgttcattta tatgattctc taaaacactg atattattgt agtactatag
    22441 attatattat tcgtagagta aagtttaaat atatgtataa agatagataa actgcacttc
    22501 aaacaagtgt gacaaaaaaa atatgtggta attttttata acttagacat gcaatgctca
    22561 ttatctctag agaggggcac gaccgggtca cgctgcacct ccttactgcc tgagacccaa
    22621 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
    22681 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
    22741 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
    22801 gataacaatt tcacacatac tagagaaaga ggagaaatac tagatggctt cctccgagga
    22861 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga
    22921 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact
    22981 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca
    23041 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc
    23101 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaggacggtg gtgttgttac
    23161 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg
    23221 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc
    23281 caccgaacgt atgtacccgg aggacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa
    23341 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc
    23401 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga
    23461 ggactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta
    23521 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag
    23581 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
    23641 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggtc tcaggga
//
