LOCUS       sequence-413998-       11236 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11236)
  AUTHORS   Nuttle X, Burt ND, Currall B, Moyses-Oliveira M, Mohajeri K, Bhavsar
            R, Lucente D, Yadav R, Tai DJC, Gusella JF, Talkowski ME
  TITLE     Parallelized engineering of mutational models using piggyBac 
            transposon delivery of CRISPR libraries.
  JOURNAL   Cell Rep Methods. 2023 Dec 5:100672. doi: 
            10.1016/j.crmeth.2023.100672.
  PUBMED    38091988
REFERENCE   2  (bases 1 to 11236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep 
            Methods. 2023 Dec 5:100672. doi: 10.1016/j.crmeth.2023.100672."
COMMENT     Sequence Label: pPB758
FEATURES             Location/Qualifiers
     source          1..11236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   5..67
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        547..925
                     /label=TRE3G promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G"
     protein_bind    555..573
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    591..609
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    627..645
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    663..681
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    699..717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    735..753
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    771..789
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     834..858
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      959..968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             971..991
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2126..2146
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2156..2212
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2216..2236
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2954..2974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2984..3037
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3041..3061
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4577..4597
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4652..4671)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4696..4744
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        4975..5186
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5199..5467
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             5501..6244
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             6245..6298
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6299..6898
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6299..6318)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6795..6815
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6931..7052
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6968..6987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7022..7041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   7821..7855
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8346..8362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8346..8362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8359..8381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8370..8386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8394..8400,8401..8418,8419..8424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8439..8460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8577..8594)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8748..9336)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8828..8847)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9507..10298,10299..10367))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10130..10149
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10368..10472)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(10498..10953)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10635..10656)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10847..10866
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11080..11102
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11094..11111
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11095..11111
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttaaccctag aaagataatc atattgtgac gtacgttaaa gataatcatg tgtaaaattg
       61 acgcatgtgt tttatcggtc tgtatatcga ggtttattta ttaatttgaa tagatattaa
      121 gttttattat atttacactt acatactaat aataaattca acaaacaatt tatttatgtt
      181 tatttattta ttaaaaaaaa caaaaactca aaatttcttc tataaagtaa caaaactttt
      241 atgagggaca gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg
      301 gggcagcagc gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag
      361 ccggcagcgt gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg
      421 aacgcttctc gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaggcctc
      481 cacggccaga ctagaggtac ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
      541 cagctcgagt ttactcccta tcagtgatag agaacgtatg aagagtttac tccctatcag
      601 tgatagagaa cgtatgcaga ctttactccc tatcagtgat agagaacgta taaggagttt
      661 actccctatc agtgatagag aacgtatgac cagtttactc cctatcagtg atagagaacg
      721 tatctacagt ttactcccta tcagtgatag agaacgtata tccagtttac tccctatcag
      781 tgatagagaa cgtataagct ttaggcgtgt acggtgggcg cctataaaag cagagctcgt
      841 ttagtgaacc gtcagatcgc ctggagcaat tccacaacac ttttgtctta taccaacttt
      901 ccgtaccact tcctaccctc gtaaaggtct agagactgga tccggtaccg aattcgccgc
      961 caccatggcc ccaaagaaga agagaaaggt gagatctgaa tccaatttca tcaacatcca
     1021 cgtgctgatc tcccactctc caagctgcct gaatagagac gatatgaaca tgcagaagga
     1081 cgccatcttt ggcggcaagc ggagagtgag gatctctagc cagtccctga agcgggccat
     1141 gagaaagtct ggctactatg cccagaatat cggcgagtcc tctctgagga ccatccacct
     1201 ggcacagctg agggacgtgc tgagacagaa gctgggcgag cggtttgatc agaagatcat
     1261 cgacaagaca ctggccctgc tgagcggcaa gtccgtggat gaggccgaga agatcagcgc
     1321 cgacgcagtg accccatggg tggtgggaga gatcgcatgg ttctgtgagc aggtggccaa
     1381 ggccgaggcc gataatctgg acgataagaa gctgctgaag gtgctgaagg aggatatcgc
     1441 cgccatcaga gtgaacctgc agcagggagt ggacatcgcc ctgagcggca ggatggccac
     1501 atccggcatg atgaccgagc tgggcaaggt ggacggagca atgtccatcg cacacgccat
     1561 caccacacac caggtggact ctgatatcga ctggttcaca gccgtggacg atctgcagga
     1621 gcagggaagc gcccacctgg gaacccagga gttcagctcc ggcgtgtttt acagatatgc
     1681 caatatcaac ctggcacagc tgcaggagaa cctgggagga gcatccaggg agcaggccct
     1741 ggagatcgcc acacacgtgg tgcacatgct ggcaaccgag gtgccaggag caaagcagcg
     1801 cacctacgcc gccttcaatc ctgccgatat ggtcatggtg aacttttccg acatgccact
     1861 gtctatggcc aatgccttcg agaaggccgt gaaggccaag gatggcttcc tgcagccttc
     1921 catccaggcc tttaaccagt actgggaccg cgtggccaat ggatatggcc tgaacggagc
     1981 tgccgcccag ttttccctgt ctgatgtgga ccctatcaca gcccaggtga agcagatgcc
     2041 aaccctggag cagctgaaga gctgggtgcg gaacaatgga gaggcaaagc ggactgctga
     2101 tggcagtgaa tttgagtcca ctagtccaaa gaagaagaga aaggtgggaa gcggagcaac
     2161 caacttcagc ctgctgaagc aggccggcga tgtggaggag aatccaggcc ccatgccaaa
     2221 gaagaagaga aaggtgacgc gtgaacgctc ctacctgatc ctgagactgg caggaccaat
     2281 gcaggcatgg ggacagccta cattcgaggg aaccaggcca acaggccgct ttcctacccg
     2341 gtctggactg ctgggactgc tgggagcctg cctgggcatc cagagggacg atacctctag
     2401 cctgcaggcc ctgagcgagt ccgtgcagtt cgccgtgcgc tgtgatgagc tgatcctgga
     2461 cgataggcgc gtgtccgtga caggcctgcg ggattaccac accgtgctgg gcgccagaga
     2521 ggactatagg ggcctgaagt cccacgagac catccagaca tggcgcgagt acctgtgcga
     2581 cgcctctttt acagtggccc tgtggctgac cccacacgca acaatggtca tcagcgagct
     2641 ggagaaggcc gtgctgaagc cacggtacac cccctatctg ggccggagaa gctgccctct
     2701 gacacaccca ctgttcctgg gcacctgtca ggcctccgat ccccagaagg ccctgctgaa
     2761 ctacgagcct gtgggcggcg acatctattc tgaggagagc gtgacaggcc accacctgaa
     2821 gttcaccgcc agggatgagc caatgatcac actgccaagg cagtttgcat ccagggagtg
     2881 gtatgtgatc aagggaggaa tggacgtgag ccagaagcgg actgctgatg gcagtgaatt
     2941 tgagtcctct agaccaaaga agaagagaaa ggtgggctcc ggcgagggca ggggaagtct
     3001 tctaacatgc ggggacgtgg aggaaaatcc cggcccaatg ccaaagaaga agagaaaggt
     3061 gggatccaac ctgctgattg acaactggat tcctgtgcgc ccacggaacg gaggaaaagt
     3121 ccagattatt aatctgcaga gcctgtactg ctcccgggat cagtggagac tgagcctgcc
     3181 cagagacgat atggagctgg ccgccctggc cctgctggtg tgcatcggcc agatcatcgc
     3241 ccctgccaag gacgatgtgg agttcaggca ccgcatcatg aaccctctga ccgaggatga
     3301 gtttcagcag ctgatcgccc catggatcga catgttctat ctgaatcacg ccgagcaccc
     3361 cttcatgcag acaaagggcg tgaaggccaa cgacgtgacc cccatggaga agctgctggc
     3421 aggcgtgtcc ggagcaacaa attgcgcctt cgtgaaccag ccaggacagg gagaggccct
     3481 gtgcggaggc tgtaccgcca tcgccctgtt taatcaggca aaccaggcac ctggattcgg
     3541 aggaggcttt aagtctggac tgaggggagg aaccccagtg accacattcg tgagaggcat
     3601 cgatctgagg agcacagtgc tgctgaatgt gctgaccctg ccacggctgc agaagcagtt
     3661 tcccaatgag agccacacag agaaccagcc cacctggatc aagcctatca agtctaacga
     3721 gagcatccct gccagctcca tcggcttcgt gagaggcctg ttttggcagc cagcccacat
     3781 cgagctgtgc gaccccatcg gcatcggcaa gtgttcttgc tgtggccagg aaagcaatct
     3841 gaggtacacc ggcttcctga aggagaagtt cacctttaca gtgaacggcc tgtggcccca
     3901 ccctcactct ccatgtctgg tgacagtgaa gaagggcgag gtggaggaga agttcctggc
     3961 ctttaccaca tccgccccct cttggaccca gatcagcaga gtggtggtgg acaagatcat
     4021 ccagaacgag aatggcaaca gagtggccgc cgtggtgaat cagttcagga acatcgcccc
     4081 acagtctccc ctggagctga tcatgggcgg ctacaggaac aatcaggcca gcatcctgga
     4141 gcggagacac gatgtgctga tgtttaatca gggctggcag cagtatggca atgtgatcaa
     4201 cgagatcgtg acagtgggcc tgggctacaa gaccgccctg agaaaggccc tgtatacatt
     4261 cgccgagggc tttaagaaca aggacttcaa gggagcaggc gtgagcgtgc acgagaccgc
     4321 cgagaggcac ttttaccgcc agtccgagct gctgatcccc gatgtgctgg ccaatgtgaa
     4381 cttctcccag gccgacgaag tgatcgccga tctgagggac aagctgcacc agctgtgcga
     4441 gatgctgttt aaccagtctg tggccccata cgcccaccac cccaagctga tcagcacact
     4501 ggccctggca agggccaccc tgtataagca cctgagggag ctgaagccac agggaggacc
     4561 ttctaatgga gctagcccaa agaagaagag aaaggtgtaa aagggttcga tccctaccgg
     4621 ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     4681 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     4741 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     4801 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     4861 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc agatctgcga
     4921 aatcggatcc agctgaatct aagtggatct gcgatcgctc cggtgcccgt cagtgggcag
     4981 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
     5041 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
     5101 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
     5161 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
     5221 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
     5281 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
     5341 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
     5401 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
     5461 acagatctaa gctgtgaccg gcgcctactc tagagccgcc atgtctagac tggacaagag
     5521 caaagtcata aactctgctc tggaattact caatggagtc ggtatcgaag gcctgacgac
     5581 aaggaaactc gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa
     5641 caagcgggcc ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactc
     5701 ctgccccctg gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg
     5761 ctgtgctctc ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga
     5821 gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct
     5881 ggagaacgca ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga
     5941 ggaacaggag catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc
     6001 cccacttctg aaacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct
     6061 tttcggcctg gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg
     6121 gccgaccgac gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga
     6181 ctttgacctt gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc
     6241 cggggagggc agaggaagtc tactaacatg cggtgacgtg gaggagaatc ccggccctat
     6301 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6361 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     6421 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6481 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6541 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6601 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6661 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6721 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6781 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6841 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa
     6901 tcaacctctg gattacaaaa tttgtgggta aacttgttta ttgcagctta taatggttac
     6961 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     7021 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggaattgact caaatgatgt
     7081 caattagtct atcagaagct catctggtct cccttccggg ggacaagaca tccctgttta
     7141 atatttaaac agcagtgttc ccaaactggg ttcttatatc ccttgctctg gtcaaccagg
     7201 ttgcagggtt tcctgtcctc acaggaacga agtccctaaa gaaacagtgg cagccaggtt
     7261 tagccccgga attgactgga ttcctttttt agggccgcgg cccattggta tggctttttc
     7321 cccgtatccc cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc
     7381 gatcccgtgc caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc
     7441 ggggggagcg ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg
     7501 agggacgtaa ttacatccct gggggctttg ggggggggct gtccctgata tctataacaa
     7561 gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa tataattatc
     7621 gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg
     7681 gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct
     7741 ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag
     7801 agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat
     7861 ctagctgcat caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg
     7921 gcgctatctg ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc
     7981 atggaaagag tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt
     8041 taccatgatg attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca
     8101 ggccacctgg gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc
     8161 gaagcgcatc agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca
     8221 gattaatgac agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg
     8281 gatgccgcag aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa
     8341 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8461 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8521 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8581 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8641 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8701 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8761 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     8821 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     8881 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     8941 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9001 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9061 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     9421 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9481 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9541 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9601 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9661 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9721 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9781 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     9841 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     9901 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     9961 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10021 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10081 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10141 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10201 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10261 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10321 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10381 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10441 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
    10501 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
    10561 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
    10621 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
    10681 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
    10741 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
    10801 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
    10861 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
    10921 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
    10981 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
    11041 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
    11101 cgacggccag tgagcgcgcc tcgttcattc acgtttttga acccgtggag gacgggcaga
    11161 ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat gaagatgctc gacacgctgc
    11221 agaacacgca gctaga
//
