LOCUS       sequence-41400-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1181
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(511..529)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        608..712
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgctcaccat ggtggcggaa gttcctatac cttttgaaga ataggaactt cggcgcgccg
       61 gagcagctct gtgccggggg ctgggtggtc tggcaggatc aaggaactgg ctaggaccgc
      121 tgcctgctcc cctcacacac tggtgctgcc ggtgctcctg caaagagaga aaccgagtgg
      181 aggggatcta ggaaggctgc agtcactcag tcccagctcc ctgataaagg ctctgggagg
      241 gtctagggga gctgagggga ggggctgaga gtcaccaatc ccagggtggc tggaggggag
      301 gcggggaggc tgtagggggg aggaggatgc agacagtagc cacacaccca gacacacaca
      361 taccccacag acccagcagt gagaccattt cctaacagca cccgagctgg ctgctgtgta
      421 tggtttgtgt acagtttgag gccggcctag gagctcgaat tcattaaggg ttattgaata
      481 tgatcggaat taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta
      541 atgtcatgat aataatggtt tcttagacgt gcggccgccg tcaggtggca cttttcgggg
      601 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      661 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      721 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      781 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      841 ttacatcgaa ctggatctca acagcggtaa ga
//
