LOCUS       sequence-414009-        7647 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP-LC3-RFP-LC3-Delta-G in mammalian cells and zebrafish 
            to measure autophagic flux.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, 
            Kodama A, Ishihara T, Mizushima T, Mizushima N
  TITLE     An Autophagic Flux Probe that Releases an Internal Control.
  JOURNAL   Mol Cell. 2016 Nov 17;64(4):835-849. doi: 
            10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3.
  PUBMED    27818143
REFERENCE   2  (bases 1 to 7647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.09.037"; journalName: "Mol Cell"; date: 
            "2016-11-17- 17"; volume: "64"; issue: "4"; pages: "835-849"
COMMENT     Sequence Label: pcDNA3-GFP-LC3-RFP-LC3-Delta-G
FEATURES             Location/Qualifiers
     source          1..7647
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(46..375)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      60..195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(114..133)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     360..380
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(389..817)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(499..520)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     711..730
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    863..1087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     1076..1093
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        1113..1131
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1113..1130
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(1601..2275)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(1601..2275)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1660..1683)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1858..1877)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2127..2145
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(join(2699..3409,3410..3412,3413..3415))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2699..3409,3410..3412,3413..3415))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2741..2762)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3090..3109
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3349..3370
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3449..3468)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3450..3468)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3513..3716)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3542..3562)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3717..4096
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4268..4287
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4362..4466
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4467..4535,4536..5327)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4685..4704)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5498..6086
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5987..6006
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6240..6257
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6374..6395
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6410..6415,6416..6433,6434..6440)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6448..6464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6453..6475
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6472..6488
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6472..6488
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6537..6658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6548..6567)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6602..6621
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6832..7626)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6943..6962)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7553..7572
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 ctcttgatca gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag
       61 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc
      121 ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg
      181 agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg
      241 catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga
      301 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
      361 ccctaactga cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca
      421 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
      481 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      541 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      601 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      661 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      721 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      781 acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag
      841 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg
      901 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac
      961 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca
     1021 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa
     1081 ggcacagtcg aggctgatca gcgagctcta gcatttaggt gacactatag aatagggccc
     1141 tctagatgca tgctcgagcg gccgccagtg tgatggatat ctgcagaatt ccagcacact
     1201 ggcggccgtt actagtggat ccttagaacg tctcctggga ggcatagacc atgtacagga
     1261 agccgtcttc atctctctcg ctctcgtaca cttcagagat gggtgtggac acactcacca
     1321 tgctgtgccc attcaccagg aggaagaagg cttggttagc attgagctgc aggcgccttc
     1381 taattatctt gatgagttcg ctcatattca cgtgatcagg tacaaggaac ttggtcttgt
     1441 ccaggacggg cagctgcttc tcacccttgt atcgctctat aatcactggg atcttggtgg
     1501 ggtgctgctc ccggatgagc cggacatctt ccactctttg ttcgaagctc cggcgctgtt
     1561 tgaaggtctt ctcggacggc atggaattca attcatcgat ggcgccggtg gagtggcggc
     1621 cctcggcgcg ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
     1681 gcttgatgtc ggtcttgtag gcgccgggca gctgcacggg cttcttggcc atgtaggtgg
     1741 tcttgacctc ggcgtcgtag tggccgccgt ccttcagctt cagcctcatc ttgatctcgc
     1801 ccttcagggc gccgtcctcg gggtacatcc gctcggtgga ggcctcccag cccatggtct
     1861 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
     1921 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     1981 agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga
     2041 tgtcggcggg gtgcttcacg taggccttgg agccgtactg gaactgaggg gacaggatgt
     2101 cccaggcgaa gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct
     2161 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
     2221 ccatgcgcac cttgaagcgc atgaactcct tgatgacgtc ctcggaggag gccatcccga
     2281 acgtctcctg ggaggcatag accatgtaca ggaagccgtc ttcatctctc tcgctctcgt
     2341 acacttcaga gatgggtgtg gacacactca ccatgctgtg cccattcacc aggaggaaga
     2401 aggcttggtt agcattgagc tgcaggcgcc ttctaattat cttgatgagt tcgctcatat
     2461 tcacgtgatc aggtacaagg aacttggtct tgtccaggac gggcagctgc ttctcaccct
     2521 tgtatcgctc tataatcact gggatcttgg tggggtgctg ctcccggatg agccggacat
     2581 cttccactct ttgttcgaag ctccggcgct gtttgaaggt cttctcggac ggcatggaat
     2641 tcaattcatc gatagatccg ccgccacccg acccaccacc gcccgagcca ccgccaccct
     2701 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     2761 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     2821 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     2881 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     2941 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     3001 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     3061 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     3121 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     3181 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     3241 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     3301 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     3361 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     3421 atccgagctc ggtaccaagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc
     3481 cagtaagcag tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt
     3541 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc
     3601 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat
     3661 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg
     3721 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     3781 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     3841 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     3901 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     3961 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     4021 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     4081 gtcaataatc aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc
     4141 ctaaccctaa gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa
     4201 attttgctcg cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt
     4261 aactatgcgg catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc
     4321 ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     4381 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4441 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4501 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4561 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4621 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4681 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4741 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4801 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4861 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4921 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4981 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5041 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5101 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     5161 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     5221 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     5281 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     5341 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     5401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5641 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6121 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     6181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     6241 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     6301 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     6361 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     6421 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     6481 gctatgacca tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag
     6541 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     6601 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg
     6661 ggtgggcgaa gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc
     6721 ggaaaacgat tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg
     6781 atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa
     6841 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     6901 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     6961 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     7021 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     7081 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     7141 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     7201 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     7261 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     7321 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     7381 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     7441 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     7501 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     7561 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     7621 aatcatgcga aacgatcctc atcctgt
//
