LOCUS       sequence-414010-        8954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a lysosomal tag (Tmem192-HA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8954)
  AUTHORS   Abu-Remaileh M, Wyant GA, Kim C, Laqtom NN, Abbasi M, Chan SH, 
            Freinkman E, Sabatini DM
  TITLE     Lysosomal metabolomics reveals V-ATPase- and mTOR-dependent 
            regulation of amino acid efflux from lysosomes.
  JOURNAL   Science. 2017 Nov 10;358(6364):807-813. doi: 
            10.1126/science.aan6298. Epub 2017 Oct 26.
  PUBMED    29074583
REFERENCE   2  (bases 1 to 8954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aan6298"; journalName: "Science"; date: 
            "2017-11-10- 10"; volume: "358"; issue: "6364"; pages: "807-813"
COMMENT     Sequence Label: pLJC5-Tmem192-3xHA
FEATURES             Location/Qualifiers
     source          1..8954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     259..278
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1274..1300
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1313..1339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1352..1378
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    1435..1552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1601..2111
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2030..2048
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2133..2732
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2133..2152)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2629..2649
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             2860..3093
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3165..3286
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3202..3221)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3256..3275
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3326..3461
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3481..3500)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3482..3500)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3510..3527)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3510..3526)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3519..3541)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3668..4123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3755..3774)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3965..3986
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4150..4253
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4254..4322,4323..5114)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4472..4491)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5285..5873
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5774..5793
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6027..6044
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6161..6182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6197..6202,6203..6220,6221..6227)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6235..6251
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6240..6262
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6259..6275
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6259..6275
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6294..6314
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6296..6314
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6342..6568
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6569..6749
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6796..6921
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7414..7647
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7832..7876
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        join(8165..8954,1..422)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
       61 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
      121 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
      181 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
      241 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
      301 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
      361 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
      421 acgaagcgct agcgctaccg gtaccatggc ggcggggggc aggatggagg acggttcctt
      481 ggatatcacc cagagtattg aagacgaccc acttctggat gcccagcttc tcccacacca
      541 ctcattacaa gctcacttta gaccccgatt ccatcctctt cctacagtca tcatagtgaa
      601 tcttctgtgg tttattcatc tcgtgtttgt tgttttagca tttttaacag gtgtgctttg
      661 ttcttatcct aatccaaatg aggacaagtg cccaggaaat tacacaaacc cattgaaagt
      721 tcagacggtt ataatccttg ggaaagttat tttgtggatt ctccatttac tccttgaatg
      781 ctacatccag tatcaccaca gcaaaatcag aaaccgaggc tataacttga tctaccgatc
      841 aacaaggcat ctcaagagac ttgcgttgat gatacagtcc tctggcaaca cagtgcttct
      901 cctcatactg tgcatgcagc actccttccc agagcctggc agattgtatc ttgacctcat
      961 tctggccatc ttggcactgg aactcatctg ttccctgata tgtctcctca tttacacagt
     1021 gaaaatccgg agatttaata aagctaaacc agagcctgat atacttgaag aagaaaaaat
     1081 ctatgcttac cccagcaata ttacctcgga gactggattc agaactattt caagcctaga
     1141 agaaattgtt gaaaagcaag gagacaccat tgaatacctg aagcgacaca atgcgctgct
     1201 gagtaagcga ttgttggctc tcacttcctc agacctgggc tgtcagccaa gtagaacggg
     1261 aggtggaggt agttatccct atgacgtgcc tgattacgcc ggcacaggat cctaccccta
     1321 tgatgtgcct gactacgctg gcagcgccgg atacccttat gatgtgcctg attatgctgg
     1381 agggagcggc taagaattct cgacctcgag acaaatggca gtattcatcc acaattttaa
     1441 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     1501 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     1561 ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg ttgcgccttt
     1621 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     1681 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     1741 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     1801 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     1861 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     1921 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     1981 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     2041 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     2101 tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc acggtgcgcc
     2161 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     2221 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     2281 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     2341 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     2401 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     2461 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     2521 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     2581 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     2641 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     2701 cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga
     2761 ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact tacaaggcag
     2821 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     2881 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     2941 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3001 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3061 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     3121 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     3181 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     3241 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     3301 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     3361 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     3421 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     3481 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     3541 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3601 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3661 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     3721 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     3781 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     3841 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     3901 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     3961 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     4021 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     4081 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     4141 gaaatgtgtg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4261 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4321 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4381 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4441 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4501 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4561 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4621 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4681 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4741 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4801 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4861 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4921 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4981 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5041 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5101 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5161 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5221 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5401 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5881 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5941 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6001 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6061 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     6121 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     6181 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     6241 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     6301 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     6361 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     6421 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     6481 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     6541 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     6601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6721 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     6781 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     6841 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     6901 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     6961 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     7021 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     7081 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     7141 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     7201 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagca
     7261 gcagctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     7321 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     7381 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     7441 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     7501 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     7561 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     7621 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     7681 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     7741 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     7801 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     7861 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     7921 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     7981 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     8041 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     8101 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagc ctcgagcggg
     8161 atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc
     8221 gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg
     8281 acagcggccc gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt
     8341 ttaggacggg acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc
     8401 gaggaaaagt agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc
     8461 cgatgattat ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt
     8521 gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg
     8581 ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga
     8641 ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg
     8701 agcgcagcaa aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct
     8761 gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg
     8821 ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga
     8881 ccctgacgtg aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg
     8941 cagttatggc ggtg
//
