LOCUS       sequence-41403-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Mills E, Truong K
  TITLE     Engineering Ca2+/calmodulin-mediated modulation of protein 
            translocation by overlapping binding and signaling peptide 
            sequences.
  JOURNAL   Cell Calcium. 2010 Apr;47(4):369-77. doi: 
            10.1016/j.ceca.2010.01.008. Epub 2010 Feb 18.
  PUBMED    20167369
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ceca.2010.01.008"; journalName: "Cell Calcium"; date: 
            "2010-04"; volume: "47"; issue: "4"; pages: "369-77"
COMMENT     Sequence Label: IELMV
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(468..577)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     protein_bind    593..617
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(617..636)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(618..636)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(678..697)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 ggcgaagcac tgcaggccgt agcccagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatca gcttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccagt ttaaacaggc tagcaatttt
      301 tttaaaccga ttcgccgcag ataccgcgat gaagttcttc cgtagttttt tccgaatccg
      361 actagcgtct agcgctacat aacatagttt ttctttaatg tcataacccc gtacataccg
      421 ttgtacagta atcgccgccc gactagtccc catggtatat ctcctttgat tgtaaataaa
      481 atgtaattta cagtatagta ttttaattaa tatacaaatg atttgataat aattcttatt
      541 taactataat atattgtgtt gggttgaatt aaaggtcccg gattaactcc ggggaattgt
      601 tatccgctca caattcccct atagtgagtc gtattaattt cggtccgatc caattctttg
      661 ccaaaatgat gagacagcac aataaccagc acgttgccca ggagctgt
//
