LOCUS       sequence-414038-       10205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of SYPB::CRY2olig(535) in neurons of Danio rerio.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10205)
  AUTHORS   Vettkotter D, Schneider M, Goulden BD, Dill H, Liewald J, Zeiler S, 
            Guldan J, Ates YA, Watanabe S, Gottschalk A
  TITLE     Rapid and reversible optogenetic silencing of synaptic transmission 
            by clustering of synaptic vesicles.
  JOURNAL   Nat Commun. 2022 Dec 19;13(1):7827. doi: 10.1038/s41467-022-35324-z.
  PUBMED    36535932
REFERENCE   2  (bases 1 to 10205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-19"; volume: "13(1):7827. doi"; pages: " 
            10.1038/s41467-022-35324-z"
COMMENT     Sequence Label: pHD57 [Tol2-UAS:sypb-egfp-cry2-polyA]
FEATURES             Location/Qualifiers
     source          1..10205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    53..187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(489..1202,1203..1205,1206..1208))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(492..1202,1203..1205,1206..1208))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(2431..2618,2619..2630)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2664..2680)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2717..2735)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2756..2772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2769..2791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2780..2796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2804..2810,2811..2828,2829..2834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2849..2870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2987..3004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3158..3746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3238..3257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3917..4708,4709..4777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4540..4559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4778..4882)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5045..5066)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5519..5530,5531..5693)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6066..6082
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6102..6122
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6124..6142
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6167..6183
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6168..6184
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6534..6550)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6587..6605)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6605..6629
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      6660..6669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7563..7565,7566..7568,7569..8279)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7563..7565,7566..7568,7569..8279)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     protein_bind    complement(9975..9999)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10001..10135
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(join(10198..10205,1..13))
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga
       61 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      241 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
      301 tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      421 gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga ggctcgagag
      481 gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      541 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      601 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      661 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      721 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      781 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      841 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      901 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      961 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
     1021 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     1081 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     1141 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     1201 ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac tggcttacta
     1261 atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt ctgcagtgtt
     1321 tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc tgataactgc
     1381 acaagtgctc agattccagc agggtggaaa atgagataaa gtgtgcagat ggggaggggg
     1441 acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa atagcccctt
     1501 tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta cctgtaaatg
     1561 atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac aaattcttaa
     1621 ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt gaattttctg
     1681 gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga catggaatga
     1741 agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa aataagagtg
     1801 aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct tgtacaaaca
     1861 gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt gcttggatgt
     1921 atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc taaagttaca
     1981 actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat gttaagtctg
     2041 ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca caactgattt
     2101 aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
     2161 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
     2221 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
     2281 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac
     2341 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt
     2401 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg
     2461 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag
     2521 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa
     2581 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc
     2641 ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga
     2701 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     2761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     2881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3781 ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca aaaaggatct
     3841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     4921 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4981 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     5041 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     5101 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     5161 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     5221 ttgacgggga aaggctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc
     5281 aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg
     5341 aatggagact acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac
     5401 ccaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
     5461 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     5521 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     5581 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
     5641 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
     5701 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
     5761 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
     5821 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
     5881 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
     5941 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     6001 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc
     6061 tcgagcagga aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt
     6121 tggtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggt
     6181 atcgataagc ttaggcctcc aaggcggagt actgtcctcc gggctggcgg agtactgtcc
     6241 tccggcaagg tcggagtact gtcctccgac actagaggtc ggagtactgt cctccgacgc
     6301 aaggcggagt actgtcctcc gggctgcgga gtactgtcct ccggcaaggt cggagtactg
     6361 tcctccgaca ctagaggtcg gagtactgtc ctccgacgca aggcggagta ctgtcctccg
     6421 ggctggcgga gtactgtcct ccggcaaggg tcgactctag agggtatata atggatccca
     6481 tcgcgtctca gcctcacttt gagctcctcc acacgaattc ctgcagcccg ggggatccac
     6541 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     6601 taattcaagt ttgtacaaaa aagcaggctc gatcgactag tttggatatc catggatccg
     6661 ccaccatgga tgttgccaac cagttggtcg ccactgggca gtttacaatc gtcaaacagc
     6721 cattaggatt catcaaaatc ttacaatggt tctttgccat cttcgcgttc gccacctgcg
     6781 gtggttactc tggagtgttt cgtatgagcg tggagtgtga gaaccgctca gagagtgacc
     6841 tgaacattga ggttgagttt gcgtacccgt tcagactgca ccaggtatgg tttgatgccc
     6901 ctacgtgtaa aggatcagaa cccgagcgac tctttctggt gggagacaac tcttcctcag
     6961 cagagttctt cgtcaccatc ggcgtcttct ccttccttta ttcaatggca gccatttctg
     7021 tgtacatctt cctgttggag aaataccgcg agggaaacaa aggagctcag gctgatttca
     7081 ttgtgacagc ggttttcacc ttcatgtggc tggtgagttc atcggcgtgg gctaaaggtc
     7141 tctcagacgt gaagagagcc accgaccctg atgacgtcat caacctcatc cctgcgtgcg
     7201 aacgagagga aaaccgatgc aaagagctgc acgaaccggt ggtgtctggc ctgaacacat
     7261 ctgtggcatt cgggttctgt aatgtgatcc tatggggagg caacctctgg ttcatcttca
     7321 aggaaaccgg ctggatggcg gctttcgctg gcacctacat gccatctgga gagaagcagc
     7381 cagctccaga ctccttcggc cagggctatg gacaggaagg ttatggacag gaccagtatg
     7441 ccagctctca gggtggatat cagcctgact acggccagca gggtggatat gaaggcggtg
     7501 gatacaatca aggagtgtat ggtcagggag agcccacatc cttctccaac gagatggaat
     7561 tcatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     7621 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     7681 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     7741 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     7801 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     7861 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     7921 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     7981 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     8041 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     8101 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     8161 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     8221 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaaga
     8281 gcggccgcgg tggcggtgga tcaggcggtg gtggatctgg cggtggtggg agcggggtac
     8341 cggtagaaaa aatgaagatg gataaaaaga caatagtgtg gttcagaaga gatttgagga
     8401 ttgaagacaa ccctgcactt gcagcagctg ctcatgaggg aagcgtgttc cctgttttta
     8461 tttggtgtcc tgaggaggag ggacaattct accctggaag agcttctaga tggtggatga
     8521 aacagtctct cgctcatctt agccagagtc tcaaggctct cggaagcgat ctcacactta
     8581 ttaaaactca taatacgatt tctgcgattc tcgactgtat tcgggtgact ggagctacaa
     8641 aagttgtttt caatcatctc tacgaccctg tgtcgcttgt gcgggatcac actgtaaagg
     8701 aaaagttggt cgagcgcggg attagcgtcc agagttataa cggagacttg ctctacgagc
     8761 cgtgggaaat atattgcgag aaggggaaac ctttcacgag ttttaacagc tattggaaga
     8821 aatgccttga catgtcgatt gagagtgtga tgctccctcc tccttggcgg ctcatgccta
     8881 taacagcagc ggctgaggcg atttgggcgt gttcgattga ggagttgggg ttggaaaacg
     8941 aagcggaaaa accgagtaac gcgctcctta caagagcttg gagccctgga tggagtaacg
     9001 ctgacaagct tttgaacgaa tttattgaaa agcaactcat agactacgca aagaatagta
     9061 agaaagtggt ggggaacagc acatctttgc tcagcccgta cctccacttt ggggagataa
     9121 gtgttagaca tgtgtttcaa tgtgcgcgga tgaaacagat tatatgggca agagacaaga
     9181 atagtgaggg agaggagagt gcagacctct tcctcagggg aattggactt agagaataca
     9241 gccggtacat atgttttaat tttccgttca cacatgaaca gtcgctcctc agtcacctcc
     9301 ggttctttcc ttgggacgct gacgtggaca agtttaaggc gtggagacag gggaggactg
     9361 gatacccgct cgtcgacgcg ggaatgagag aactctgggc tactggatgg atgcacaata
     9421 gaataagagt cattgtgtct agtttcgctg tcaagttcct cctcctccct tggaaatggg
     9481 gaatgaagta cttttgggac acactcctcg acgctgacct cgagtgtgat attctcgggt
     9541 ggcaatacat tagcgggagt attccggacg ggcatgaact cgaccggctc gataacccgg
     9601 cgcttcaggg ggcgaaatac gatcctgagg gagaatatat aaggcagtgg ctcccggaac
     9661 tcgcgagact ccctacagag tggattcacc acccttggga tgctcctctt actgtactca
     9721 aagctagcgg agtcgagctc ggaacaaatt acgcgaaacc gattgtagat attgatacag
     9781 ctcgcgaatt gttggctaaa gctatttcta gaactcgcgg agcacaaatt atgattggag
     9841 cagcacctga cgaaattgta gcagacagtt ttgaagcgct tggggctaac actattaaag
     9901 agcctggact ctgccctagc gtcagcagca acgatcagca ggtaccttcg gctgtgtaac
     9961 gatcgactag tcgaacccag ctttcttgta caaagtgggg gatccagaca tgataagata
    10021 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
    10081 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
    10141 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttccaa
    10201 cttta
//
