LOCUS       sequence-41406-e         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Kobayashi T, Ooms LS, Ikizler M, Chappell JD, Dermody TS
  TITLE     An improved reverse genetics system for mammalian orthoreoviruses.
  JOURNAL   Virology. 2010 Mar 15;398(2):194-200. Epub 2009 Dec 29.
  PUBMED    20042210
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2010-03-15"; volume: "398(2)"; pages: "194-200. Epub 2009 Dec
            29"
COMMENT     Sequence Label: pT7-L2-M3T1L
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(307..325)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      321..368
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(436..454)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(437..454)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcatacttaa tagtcaggtt ttgtgccacc tcaggattgg gatgttctta ccgatactat
       61 tagctggtct ccttcgctcc caacttatgt ggtgcctccg ggtgattata ctctgacacc
      121 tctgtaactc attacccctc gtgagcgtgc ctaattcatc gggtcggcat ggcatctcca
      181 cctcctcgcg gtccgacctg ggcatccgaa ggaggacgtc gtccactcgg atggctaagg
      241 gagagctcaa aaaaaaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg
      301 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
      361 gttttttgct gaaaggagga actatatccg gatcgagatc ctctagagtc gacctgcagg
      421 catgcaagct tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
      481 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
      541 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
      601 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
      661 cagcagggtc atcgctagct aatacgactc actatagcta aagtgaccgt ggtcatggct
      721 tcattcaagg gattctccgt caacactgtt ccagtttcca aggccaaacg tgatatatca
      781 tctcttg
//
