LOCUS       sequence-414079-        6180 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-ABL1
FEATURES             Location/Qualifiers
     source          1..6180
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(127..154)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(246..332)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     381..403
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     395..412
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     396..412
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    428..527
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3918..4017)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4034..4053)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4035..4053)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4058..4074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4058..4074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4505..5296
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5389..5977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5878..5897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6131..6148
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
       61 agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag agtttgtaga
      121 aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct ggcagtttat
      181 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa atccgctccc
      241 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
      301 cagtcttccg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt
      361 aacgctagca tggatgtttt cccagtcacg acgttgtaaa acgacggcca gtcttaagct
      421 cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
      481 tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gttggcacca tgttggagat
      541 ctgcctgaag ctggtgggct gcaaatccaa gaaggggctg tcctcgtcct ccagctgtta
      601 tctggaagaa gcccttcagc ggccagtagc atctgacttt gagcctcagg gtctgagtga
      661 agccgctcgt tggaactcca aggaaaacct tctcgctgga cccagtgaaa atgaccccaa
      721 ccttttcgtt gcactgtatg attttgtggc cagtggagat aacactctaa gcataactaa
      781 aggtgaaaag ctccgggtct taggctataa tcacaatggg gaatggtgtg aagcccaaac
      841 caaaaatggc caaggctggg tcccaagcaa ctacatcacg ccagtcaaca gtctggagaa
      901 acactcctgg taccatgggc ctgtgtcccg caatgccgct gagtatctgc tgagcagcgg
      961 gatcaatggc agcttcttgg tgcgtgagag tgagagcagt cctggccaga ggtccatctc
     1021 gctgagatac gaagggaggg tgtaccatta caggatcaac actgcttctg atggcaagct
     1081 ctacgtctcc tccgagagcc gcttcaacac cctggccgag ttggttcatc atcattcaac
     1141 ggtggccgac gggctcatca ccacgctcca ttatccagcc ccaaagcgca acaagcccac
     1201 tgtctatggt gtgtccccca actacgacaa gtgggagatg gaacgcacgg acatcaccat
     1261 gaagcacaag ctgggcgggg gccagtacgg ggaggtgtac gagggcgtgt ggaagaaata
     1321 cagcctgacg gtggccgtga agaccttgaa ggaggacacc atggaggtgg aagagttctt
     1381 gaaagaagct gcagtcatga aagagatcaa acaccctaac ctggtgcagc tccttggggt
     1441 ctgcacccgg gagcccccgt tctatatcat cactgagttc atgacctacg ggaacctcct
     1501 ggactacctg agggagtgca accggcagga ggtgaacgcc gtggtgctgc tgtacatggc
     1561 cactcagatc tcgtcagcca tggagtacct ggagaagaaa aacttcatcc acagagatct
     1621 tgctgcccga aactgcctgg taggggagaa ccacttggtg aaggtagctg attttggcct
     1681 gagcaggttg atgacagggg acacctacac agcccatgct ggagccaagt tccccatcaa
     1741 atggactgca cccgagagcc tggcctacaa caagttctcc atcaagtccg acgtctgggc
     1801 atttggagta ttgctttggg aaattgctac ctatggcatg tccccttacc cgggaattga
     1861 cctgtcccag gtgtatgagc tgctagagaa ggactaccgc atggagcgcc cagaaggctg
     1921 cccagagaag gtctatgaac tcatgcgagc atgttggcag tggaatccct ctgaccggcc
     1981 ctcctttgct gaaatccacc aagcctttga aacaatgttc caggaatcca gtatctcaga
     2041 cgaagtggaa aaggagctgg ggaaacaagg cgtccgtggg gctgtgagta ccttgctgca
     2101 ggccccagag ctgcccacca agacgaggac ctccaggaga gctgcagagc acagagacac
     2161 cactgacgtg cctgagatgc ctcactccaa gggccaggga gagagcgatc ctctggacca
     2221 tgagcctgcc gtgtctccat tgctccctcg aaaagagcga ggtcccccgg agggcggcct
     2281 gaatgaagat gagcgccttc tccccaaaga caaaaagacc aacttgttca gcgccttgat
     2341 caagaagaag aagaagacag ccccaacccc tcccaaacgc agcagctcct tccgggagat
     2401 ggacggccag ccggagcgca gaggggccgg cgaggaagag ggccgagaca tcagcaacgg
     2461 ggcactggct ttcaccccct tggacacagc tgacccagcc aagtccccaa agcccagcaa
     2521 tggggctggg gtccccaatg gagccctccg ggagtccggg ggctcaggct tccggtctcc
     2581 ccacctgtgg aagaagtcca gcacgctgac cagcagccgc ctagccaccg gcgaggagga
     2641 gggcggtggc agctccagca agcgcttcct gcgctcttgc tccgcctcct gcgttcccca
     2701 tggggccaag gacacggagt ggaggtcagt cacgctgcct cgggacttgc agtccacggg
     2761 aagacagttt gactcgtcca catttggagg gcacaaaagt gagaagccgg ctctgcctcg
     2821 gaagagggca ggggagaaca ggtctgacca ggtgacccga ggcacagtaa cgcctccccc
     2881 caggctggtg aaaaagaatg aggaagctgc tgatgaggtc ttcaaagaca tcatggagtc
     2941 cagcccgggc tccagcccgc ccaacctgac tccaaaaccc ctccggcggc aggtcaccgt
     3001 ggcccctgcc tcgggcctcc cccacaagga agaagctgga aagggcagtg ccttagggac
     3061 ccctgctgca gctgagccag tgacccccac cagcaaagca ggctcaggtg caccaggggg
     3121 caccagcaag ggccccgccg aggagtccag agtgaggagg cacaagcact cctctgagtc
     3181 gccagggagg gacaagggga aattgtccag gctcaaacct gccccgccgc ccccaccagc
     3241 agcctctgca gggaaggctg gaggaaagcc ctcgcagagc ccgagccagg aggcggccgg
     3301 ggaggcagtc ctgggcgcaa agacaaaagc cacgagtctg gttgatgctg tgaacagtga
     3361 cgctgccaag cccagccagc cgggagaggg cctcaaaaag cccgtgctcc cggccactcc
     3421 aaagccacag tccgccaagc cgtcggggac ccccatcagc ccagcccccg ttccctccac
     3481 gttgccatca gcatcctcgg ccctggcagg ggaccagccg tcttccaccg ccttcatccc
     3541 tctcatatca acccgagtgt ctcttcggaa aacccgccag cctccagagc ggatcgccag
     3601 cggtgccatc accaagggcg tggtcctgga cagcaccgag gcgctgtgcc tcgccatctc
     3661 taggaactcc gagcagatgg ccagccacag cgcagtgctg gaggccggca aaaacctcta
     3721 cacgttctgc gtgagctatg tggattccat ccagcaaatg aggaacaagt ttgccttccg
     3781 agaggccatc aacaaactgg agaataatct ccgggagctt cagatctgcc cggcgacagc
     3841 aggcagtggt ccagcggcca ctcaggactt cagcaagctc ctcagttcgg tgaaggaaat
     3901 cagtgacata gtgcagaggc caactttctt gtacaaagtt ggcattataa gaaagcattg
     3961 cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca
     4021 tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct
     4081 ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     4141 cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca aggttgccgg
     4201 gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag cctgttcggt
     4261 tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga
     4321 acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact gtttttttgg
     4381 ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt
     4441 gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa caaagttaaa
     4501 cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     4561 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     4621 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     4681 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     4741 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     4801 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     4861 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     4921 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     4981 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     5041 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     5101 cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     5161 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     5221 ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg agatcaccaa
     5281 ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg tgagttacgc
     5341 gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5401 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5461 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5521 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5581 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5641 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5701 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5761 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5821 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5881 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5941 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6001 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6061 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6121 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
//
