LOCUS       sequence-414083-        8691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To reconstitute CD20 expression with a specific mRNA isoform 
            (variant 2) after CRISPR-Cas9-mediated knockout of CD20..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8691)
  AUTHORS   Ang Z, Paruzzo L, Hayer KE, Schmidt C, Torres-Diz M, Xu F, Zankharia
            U, Zhang Y, Soldan SS, Zheng S, Falkenstein CD, Loftus JP, Yang SY, 
            Asnani M, King Sainos P, Pillai V, Chong ER, Li M, Tasian SK, Barash
            Y, Lieberman PM, Ruella M, Schuster SJ, Thomas-Tikhonenko A
  TITLE     Alternative splicing of its 5' UTR limits CD20 mRNA translation and 
            enables resistance to CD20-directed immunotherapies.
  JOURNAL   Blood. 2023 Sep 8:blood.2023020400. doi: 10.1182/blood.2023020400.
  PUBMED    37683180
REFERENCE   2  (bases 1 to 8691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Sep 8:blood.2023020400. doi: 10.1182/blood.2023020400."
COMMENT     Sequence Label: pCDH-V2-rCD20-BSD
FEATURES             Location/Qualifiers
     source          1..8691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1966..2177
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2190..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             complement(2676..2687)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3779..4348
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3798..4350
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3942..3959)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4169..4188
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4362..4757
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4770..5358
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4823..4843)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5241..5252)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5432..5665
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5737..5858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5774..5793)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5828..5847
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5898..6033
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6066..6082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6066..6082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6079..6101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6090..6106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6114..6120,6121..6138,6139..6144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6159..6180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6297..6314)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6468..7056)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7227..8018,8019..8087))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7850..7869
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8088..8192)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8260..8278
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8316..8338)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8438..8457
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8651..8673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8665..8682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8666..8682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     2461 agctgtgacc ggcgcctact ctagaggtct atcagcgatt tcatcttcag gcctggacta
     2521 caccactcac cctcccagtg tgcttgagaa acaaactgca cccactgaac tccgcagcta
     2581 gcatccaaat cagcccttga gatttgaggc cttggagact cagaactcag cagtaggcct
     2641 tgcctcagat ccaaggtcac tcggaagagg ccatgtctac cctcaatgac actcatggag
     2701 gaaatgctga gagaagcatt cagatgcatg acacaaggta agactgccaa aaatcttgtt
     2761 cttgctctcc tcattttgtt atttgtttta tttttaggag ttttgagagc aaaatgacaa
     2821 cacccagaaa ttcagtaaat gggactttcc cggcagagcc aatgaaaggc cctattgcta
     2881 tgcaatctgg tccaaaacca ctcttcagga ggatgtcttc actggtgggc cccacgcaaa
     2941 gcttcttcat gagggaatct aagactttgg gggctgtcca gattatgaat gggctcttcc
     3001 acattgcgtt gggcggacta cttatgattc cagcagggat ctatgcaccc atctgtgtga
     3061 ctgtgtggta ccctctctgg ggaggcatta tgtatattat ttccggatca ctcctggcag
     3121 caacggagaa aaactccagg aagtgtttgg tcaaaggaaa aatgataatg aattcattga
     3181 gcctctttgc tgccatttct ggaatgattc tttcaatcat ggacatactt aatattaaaa
     3241 tttcccattt tttaaaaatg gagagtctga attttattag agctcacaca ccatatatta
     3301 acatatacaa ctgtgaacca gctaatccct ctgagaaaaa ctccccatct acccaatact
     3361 gttacagcat acaatctctg ttcttgggca ttttgtcagt gatgctgatc tttgccttct
     3421 tccaggaact tgtaatagct ggcatcgttg agaatgaatg gaaaagaacg tgctccagac
     3481 ccaaatctaa catagttctc ctgtcagcag aagaaaaaaa agaacagact attgaaataa
     3541 aagaagaagt ggttgggcta actgaaacat cttcccaacc aaagaatgaa gaagacattg
     3601 aaattattcc aatccaagaa gaggaagaag aagaaacaga gacgaacttt ccagaacctc
     3661 cccaagatca ggaatcctca ccaatagaaa atgacagctc tccttaagga tcccagtgtg
     3721 gtggtacggg aattcctgca ggcctcgagg gccggcgcgc cgcggccgct acgtaaattc
     3781 cgcccccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3841 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3901 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3961 aggtcttttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4021 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgt
     4081 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4141 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4201 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     4261 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     4321 ggttttcctt tgaaaaacac gatgataata tggccacaac catggttcct ttgtctcaag
     4381 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     4441 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     4501 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     4561 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     4621 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     4681 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     4741 atgtgtggga gggctaaatc taggtcgaca atcaacctct ggattacaaa atttgtgaaa
     4801 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     4861 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     4921 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     4981 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     5041 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     5101 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     5161 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     5221 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     5281 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     5341 tttgggccgc ctccccgcct ggtaccttta agaccaatga cttacaaggc agctgtagat
     5401 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5461 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5521 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5581 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5641 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     5701 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5761 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5821 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5881 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5941 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     6001 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac caaattcgta
     6061 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6121 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6181 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6241 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6301 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6361 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6421 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6481 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6541 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6601 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6661 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6721 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6781 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6841 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6901 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6961 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7021 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7081 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7141 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7201 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7261 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7321 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7381 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7441 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7501 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7561 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7621 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7681 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7741 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7801 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7861 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7921 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7981 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8041 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8101 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8161 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8221 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8281 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8341 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8401 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     8461 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     8521 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8581 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8641 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//
