LOCUS       sequence-414087-        8780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To reconstitute CD20 expression with a specific mRNA isoform 
            (variant 1) after CRISPR-Cas9-mediated knockout of CD20..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8780)
  AUTHORS   Ang Z, Paruzzo L, Hayer KE, Schmidt C, Torres-Diz M, Xu F, Zankharia
            U, Zhang Y, Soldan SS, Zheng S, Falkenstein CD, Loftus JP, Yang SY, 
            Asnani M, King Sainos P, Pillai V, Chong ER, Li M, Tasian SK, Barash
            Y, Lieberman PM, Ruella M, Schuster SJ, Thomas-Tikhonenko A
  TITLE     Alternative splicing of its 5' UTR limits CD20 mRNA translation and 
            enables resistance to CD20-directed immunotherapies.
  JOURNAL   Blood. 2023 Sep 8:blood.2023020400. doi: 10.1182/blood.2023020400.
  PUBMED    37683180
REFERENCE   2  (bases 1 to 8780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2023
            Sep 8:blood.2023020400. doi: 10.1182/blood.2023020400."
COMMENT     Sequence Label: pCDH-V1-rCD20-BSD
FEATURES             Location/Qualifiers
     source          1..8780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1966..2177
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             2190..2458
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             complement(2765..2776)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3868..4437
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3887..4439
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4031..4048)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4258..4277
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4451..4846
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4859..5447
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4912..4932)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5330..5341)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5521..5754
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5826..5947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5863..5882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5917..5936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5987..6122
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6155..6171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6155..6171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6168..6190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6179..6195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6203..6209,6210..6227,6228..6233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6248..6269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6386..6403)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6557..7145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7316..8107,8108..8176))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7939..7958
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8177..8281)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8349..8367
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8405..8427)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8527..8546
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8740..8762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8754..8771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8755..8771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     1981 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     2041 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2101 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2161 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     2221 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     2281 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     2341 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     2401 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     2461 agctgtgacc ggcgcctact ctagaggtct atcagcgatt tcatcttcag gcctggacta
     2521 caccactcac cctcccagtg tgcttgagaa acaaactgca cccactgaac tccgcagcta
     2581 gcatccaaat cagcccttga gatttgaggc cttggagact cagatcctga acaagagaga
     2641 acaaaatctc tactttgatg gaacttccat tctgtgggga agagactgac aataagcaat
     2701 taaataaata agaactcagc agtaggcctt gcctcagatc caaggtcact cggaagaggc
     2761 catgtctacc ctcaatgaca ctcatggagg aaatgctgag agaagcattc agatgcatga
     2821 cacaaggtaa gactgccaaa aatcttgttc ttgctctcct cattttgtta tttgttttat
     2881 ttttaggagt tttgagagca aaatgacaac acccagaaat tcagtaaatg ggactttccc
     2941 ggcagagcca atgaaaggcc ctattgctat gcaatctggt ccaaaaccac tcttcaggag
     3001 gatgtcttca ctggtgggcc ccacgcaaag cttcttcatg agggaatcta agactttggg
     3061 ggctgtccag attatgaatg ggctcttcca cattgcgttg ggcggactac ttatgattcc
     3121 agcagggatc tatgcaccca tctgtgtgac tgtgtggtac cctctctggg gaggcattat
     3181 gtatattatt tccggatcac tcctggcagc aacggagaaa aactccagga agtgtttggt
     3241 caaaggaaaa atgataatga attcattgag cctctttgct gccatttctg gaatgattct
     3301 ttcaatcatg gacatactta atattaaaat ttcccatttt ttaaaaatgg agagtctgaa
     3361 ttttattaga gctcacacac catatattaa catatacaac tgtgaaccag ctaatccctc
     3421 tgagaaaaac tccccatcta cccaatactg ttacagcata caatctctgt tcttgggcat
     3481 tttgtcagtg atgctgatct ttgccttctt ccaggaactt gtaatagctg gcatcgttga
     3541 gaatgaatgg aaaagaacgt gctccagacc caaatctaac atagttctcc tgtcagcaga
     3601 agaaaaaaaa gaacagacta ttgaaataaa agaagaagtg gttgggctaa ctgaaacatc
     3661 ttcccaacca aagaatgaag aagacattga aattattcca atccaagaag aggaagaaga
     3721 agaaacagag acgaactttc cagaacctcc ccaagatcag gaatcctcac caatagaaaa
     3781 tgacagctct ccttaaggat cccagtgtgg tggtacggga attcctgcag gcctcgaggg
     3841 ccggcgcgcc gcggccgcta cgtaaattcc gccccccccc cccctaacgt tactggccga
     3901 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     3961 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     4021 ggtctttccc ctctcgccaa aggaatgcaa ggtcttttga atgtcgtgaa ggaagcagtt
     4081 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     4141 cccccacctg gcgacaggtg cctctgcgtc caaaagccac gtgtataaga tacacctgca
     4201 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     4261 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     4321 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     4381 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat
     4441 ggccacaacc atggttcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     4501 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     4561 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     4621 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     4681 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     4741 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     4801 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaaatct aggtcgacaa
     4861 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4921 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4981 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5041 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5101 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5161 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     5221 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     5281 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     5341 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     5401 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa
     5461 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5521 tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc
     5581 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5641 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5701 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     5761 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     5821 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     5881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     5941 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     6001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6061 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6121 cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     6181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6241 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6301 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6361 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     6601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     6661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6961 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     7621 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     7681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7921 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8281 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     8341 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     8401 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     8461 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     8521 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     8581 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     8641 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     8701 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     8761 cgacggccag tgccaagctg
//
