LOCUS       sequence-414093-        9719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral vector for expressing human B3GALT5 with C-terminal 
            myc-DDK tag, includes nucleotide change in PAM targeting sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9719)
  TITLE     Fucosylated glycoproteins and fucosylated glycolipids play opposing 
            roles in cholera intoxication
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: lentiMutB3GALT5-blast
FEATURES             Location/Qualifiers
     source          1..9719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             5..61
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             62..460
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    485..1073
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(538..558)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(956..967)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1076..1092)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1077..1093)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1598..1778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1804..1821)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1810..2034
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2080..2508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2167..2186)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2377..2398
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2517..2537)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2522..2851
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2702..2837
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2764..2783
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        2899..2946
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2965..3339
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3469..3590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3506..3525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3560..3579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3639..3655)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3639..3655)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3652..3674)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3663..3679
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3687..3693,3694..3711,3712..3717))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3732..3753
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3870..3887)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4041..4629)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4121..4140)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4800..5591,5592..5660))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5423..5442
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5661..5765)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5840..5859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6031..6410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6412..6610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6565..6585
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             6628..6808
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6855..6980
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7473..7706
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7891..7935
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8233..8350
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8443..8654
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             9643..9672
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9691..9714
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
       61 gatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
      121 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
      181 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
      241 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
      301 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
      361 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
      421 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaattcgata tcaagcttat
      481 cggtaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
      541 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
      601 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
      661 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
      721 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
      781 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
      841 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
      901 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
      961 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     1021 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     1081 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     1141 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     1201 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     1261 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     1321 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     1381 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     1441 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     1501 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     1561 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     1621 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     1681 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     1741 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     1801 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     1861 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     1921 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     1981 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     2041 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     2101 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2161 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2221 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2281 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2341 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2401 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2461 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     2521 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     2581 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     2641 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     2701 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     2761 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     2821 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     2881 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     2941 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     3001 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     3061 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     3121 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     3181 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     3241 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     3301 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     3361 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     3421 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     3481 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     3541 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     3601 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     3661 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     3721 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     3781 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     3841 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     3901 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     3961 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4021 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4081 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4141 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4201 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4261 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4321 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4381 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4441 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4501 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4561 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     4621 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     4681 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     4741 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     4801 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     4861 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     4921 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     4981 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5041 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5101 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5161 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5221 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5281 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5341 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5401 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5461 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5521 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5581 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5641 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     5701 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     5761 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     5821 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     5881 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     5941 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     6001 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     6061 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     6121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     6181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     6241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     6421 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     6481 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     6541 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     6601 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     6661 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     6721 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     6781 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     6841 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     6901 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     6961 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     7021 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     7081 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     7141 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     7201 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     7261 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     7321 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     7381 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     7441 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     7501 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     7561 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     7621 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     7681 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     7741 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     7801 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     7861 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     7921 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     7981 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     8041 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     8101 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     8161 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     8221 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     8281 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     8341 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttagctagct
     8401 aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc
     8461 ccacagtccc cgagaagttg gggggagggg tcggcaattg atccggtgcc tagagaaggt
     8521 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     8581 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     8641 ccgccagaac acaggaccgg ttctagagcg aggagatctg ccgccgcgat cgccatggct
     8701 ttcccgaaga tgagattgat gtatatttgc cttctggttc tgggggctct ttgtttgtat
     8761 tttagcatgt acagtctaaa tcctttcaaa gaacagtcct ttgtttacaa gaaagacggg
     8821 aacttcctta agctcccaga tacagactgc aggcagacac ctcccttcct cgtcctgctg
     8881 gtgacctcat cccacaaaca gttggctgag cgcatggcca tccggcagac gtgggggaaa
     8941 gagaggacgg tgaagggaaa gcagctgaag acattcttcc tcctggggac caccagcagt
     9001 gcagcggaaa caaaagaggt ggaccaggag agccagcgac acggggacat tatccagaag
     9061 gatttcctag acgtctatta caatctgacc ctgaagacca tgatgggcat agaatgggtc
     9121 catcgctttt gtcctcaggc agcgtttgtg atgaaaacag actcagacat gttcatcaat
     9181 gttgactatc tgactgaact gcttctgaag aaaaacagaa caaccaggtt tttcactggc
     9241 ttcttgaaac tcaatgagtt tcccatcagg cagccattca gcaagtggtt tgtcagtaaa
     9301 tctgaatatc cgtgggacag gtacccacca ttctgctccg gcaccggcta cgtgttttct
     9361 ggcgacgtgg cgagtcaggt gtacaatgtc tccaagagcg tcccatacat taaactggaa
     9421 gacgtgtttg tggggctctg cctcgaaagg ctgaacatca gattggagga gctccactcc
     9481 cagccgacct tttttccagg gggcttacgc ttctccgtat gcctcttcag gaggatcgtg
     9541 gcctgccact tcatcaagcc tcggactctc ttggactact ggcaggctct agagaattcc
     9601 cggggggaag attgtccgcc tgtcacgcgt acgcggccgc tcgagcagaa actcatctca
     9661 gaagaggatc tggcagcaaa tgatatcctg gattacaagg atgacgacga taagggatc
//
