LOCUS       sequence-414099-        9429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox inducible myc tag truncated SOX9 without DNA-binding domain 
            (noHMG).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9429)
  AUTHORS   Yang Y, Gomez N, Infarinato N, Adam RC, Sribour M, Baek I, Laurin M,
            Fuchs E
  TITLE     The pioneer factor SOX9 competes for epigenetic factors to switch 
            stem cell fates.
  JOURNAL   Nat Cell Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y.
  PUBMED    37488435
REFERENCE   2  (bases 1 to 9429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2023 Jul 24. doi: 10.1038/s41556-023-01184-y."
COMMENT     Sequence Label: plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
FEATURES             Location/Qualifiers
     source          1..9429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(121..140)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      934..1522
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1423..1442
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1676..1693
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1810..1831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1846..1851,1852..1869,1870..1876)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1884..1900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1889..1911
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1908..1924
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1908..1924
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1943..1963
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1945..1963
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1991..2217
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2218..2398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2445..2570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3063..3296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3481..3525
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3823..3940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4383..4401
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4419..4437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4455..4473
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4491..4509
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4527..4545
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4563..4581
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4599..4617
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4662..4686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4779..4799
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4800..4829
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        6124..6624
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6543..6561
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6633..7235
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7132..7152
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7252..7840
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7305..7325)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7723..7734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7938..8171
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8243..8364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8280..8299)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8334..8353
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8404..8539
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8559..8578)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8560..8578)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8588..8605)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8588..8604)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8597..8619)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8746..9201
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8833..8852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9043..9064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9227..9331
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9332..9400,9401..9429,1..763)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
       61 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      121 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      181 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      241 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      301 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      361 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
      421 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
      481 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
      541 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
      601 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
      661 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
      721 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
      781 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
      841 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
      901 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
      961 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1021 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1081 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1141 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1201 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1261 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1321 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1381 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1441 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     1501 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     1561 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     1621 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     1681 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     1741 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     1801 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     1861 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     1921 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     1981 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     2041 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     2101 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     2161 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     2221 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2281 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2341 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2401 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     2461 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     2521 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     2581 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     2641 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     2701 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     2761 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2821 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2881 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2941 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     3001 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     3061 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     3121 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     3181 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     3241 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     3301 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     3361 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     3421 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     3481 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     3541 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     3601 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     3661 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     3721 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     3781 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     3841 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     3901 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     3961 cagagatcca gtttggttag taccgggccc ggtgctttgc tctgagccag cccaccagtt
     4021 tggaatgact cctttttatg acttgaattt tcaagtataa agtctagtgc taaatttaat
     4081 ttgaacaact gtatagtttt tgctggttgg gggaaggaaa aaaaatggtg gcagtgtttt
     4141 tttcagaatt agaagtgaaa tgaaaacttg ttgtgtgtga ggatttctaa tgacatgtgg
     4201 tggttgcata ctgagtgaag ccggtgagca ttctgccatg tcaccccctc gtgctcagta
     4261 atgtacttta cagaaatcct aaactcaaaa gattgatata aaccatgctt cttgtgtata
     4321 tccggtctct tctctgggta gtctcactca gcctgcattt ctgccatcga tggtaccttt
     4381 actccctatc agtgatagag aacgtatgaa gagtttactc cctatcagtg atagagaacg
     4441 tatgcagact ttactcccta tcagtgatag agaacgtata aggagtttac tccctatcag
     4501 tgatagagaa cgtatgacca gtttactccc tatcagtgat agagaacgta tctacagttt
     4561 actccctatc agtgatagag aacgtatatc cagtttactc cctatcagtg atagagaacg
     4621 tataagcttt aggcgtgtac ggtgggcgcc tataaaagca gagctcgttt agtgaaccgt
     4681 cagatcgcct ggagcaattc cacaacactt ttgtcttata ccaactttcc gtaccacttc
     4741 ctaccctcgt aaagctagca ccggtcgcca ccatgagccc taagaagaaa cggaaagtgg
     4801 aacaaaaact tatttctgaa gaagatctga atctcctgga ccccttcatg aagatgaccg
     4861 acgagcagga gaagggcctg tctggcgccc ccagccccac catgtcggag gactcggctg
     4921 gttcgccctg tccctcgggc tccggctcgg acacggagaa cacccggccc caggagaaca
     4981 ccttccccaa gggcgagccg gatctgaaga aggagagcga ggaagataag ttccccgtgt
     5041 gcatccgcga ggcggtcagc caggtgctga agggctacga ctggacgctg gtgcccatgc
     5101 ccgtgcgcgt caacggctcc agcaagaaca aggtgaagaa cggacaagcg gaggccgaag
     5161 aggccacgga acagactcac atctctccta atgctatctt caaggcgctg caagccgact
     5221 ccccacattc ctcctccggc atgagtgagg tgcactcccc gggcgagcac tctgggcaat
     5281 ctcagggtcc gccgacccca cccaccactc ccaaaaccga cgtgcaagct ggcaaagttg
     5341 atctgaagcg agaggggcgc cctctggcag aggggggcag acagcccccc atcgacttcc
     5401 gcgacgtgga catcggtgaa ctgagcagcg acgtcatctc caacattgag accttcgacg
     5461 tcaatgagtt tgaccaatac ttgccaccca acggccaccc aggggttccg gccacccacg
     5521 gccaggtcac ctacactggc agttacggca tcagcagcac cgcacccacc cctgcgaccg
     5581 cgggccacgt gtggatgtcg aagcagcagg cgccgccccc tcctccgcag cagcctccgc
     5641 aggccccgca agccccacag gcgcctccgc agcagcaagc acccccgcag cagccgcagg
     5701 caccccagca gcagcaggca cacacgctca ccacgctgag cagcgagcca ggccagtccc
     5761 agcgaacgca catcaagacg gagcagctga gccccagcca ctacagcgag cagcagcagc
     5821 actccccgca acagatctcc tacagcccct tcaaccttcc tcactacagc ccctcctacc
     5881 cgcccatcac ccgctcgcaa tacgactacg ctgaccatca gaactccggc tcctactaca
     5941 gtcacgcagc cggccagggc tcagggctct actccacctt cacttacatg aaccccgcgc
     6001 agcgccccat gtacaccccc atcgctgaca cctccggggt cccttccatc ccgcagaccc
     6061 acagcccgca gcactgggaa caaccagtct acacacagct caccagaccc tgaacgcgtg
     6121 gggttgcgcc ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt
     6181 ggttccggga aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc
     6241 agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg
     6301 cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc
     6361 gcacgtctca ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga
     6421 ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg
     6481 gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg
     6541 cggtgttccg cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac
     6601 cgaatcaccg acctctctcc ccagggggat ccatggccac cgagtacaag cccacggtgc
     6661 gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg
     6721 actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc
     6781 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     6841 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     6901 ccgagatcgg ctcgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     6961 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     7021 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     7081 aggcggccga gcgcgctggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     7141 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     7201 gcacctggtg catgacccgc aagcccggtg cctgaggatc ctctagatcc taatcaacct
     7261 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7321 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7381 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7441 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7501 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7561 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7621 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7681 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7741 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7801 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
     7861 tgcatcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7921 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     7981 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8041 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8101 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8161 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     8221 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     8281 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8341 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     8401 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8461 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8521 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     8581 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8641 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8701 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     8761 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8821 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8881 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8941 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9001 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9061 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9121 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     9181 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     9241 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     9301 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     9361 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     9421 agtaaaaga
//
