LOCUS       sequence-41410-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the eGFP reporter gene was not detected in the brain 
            and eye..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1159
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(592..610)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ccttgctcac catggtggcg gaagttccta taccttttga agaataggaa cttcggcgcg
       61 cccaaagtcc agctaggctg tctgcaccag ttgctcaacc ctgctccctc agccctctct
      121 tggaaaggag gaaatgaaag tctcacttcc tgtactggag atgatcccac ccacagcctg
      181 actcaggggc ctcagctcta gccacctctc tcttagtcat tagtttttgt aattaagtta
      241 ggggactgtt ggccctgctt catcagggca aagtcaacag tgtcacatgg gtgcaaacgg
      301 agtcctaggg cagttgatcc acagggatcc atgtgtcaaa ctcagcgaaa cttgcttggc
      361 agttgtggca agtagctatg ggaacagctg aggtacaggc ccctcccttg gtggggaaat
      421 cggtttccac tcctttcgta aggaggaagg aggctgaggg gagggggccc ctgggaggga
      481 gcctgccctg ggttgctaac catctcctct ctgccaaaag ggccggccta ggagctcgaa
      541 ttcattaagg gttattgaat atgatcggaa ttaattcttg aagacgaaag ggcctcgtga
      601 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tgcggccgcc
      661 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      721 acattcaaat atgtatccgc tcatgagaca ataaccctga taaa
//
