LOCUS       sequence-414193-        5496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Homo-dimer expression vector with plasma membrane localization 
            signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Petrich A, Aji AK, Dunsing V, Chiantia S
  TITLE     Benchmarking of novel green fluorescent proteins for the 
            quantification of protein oligomerization in living cells.
  JOURNAL   PLoS One. 2023 Aug 3;18(8):e0285486. doi: 
            10.1371/journal.pone.0285486. eCollection 2023.
  PUBMED    37535571
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-08-3"; volume: "18(8):e0285486. doi"; pages: " 
            10.1371/journal.pone.0285486. eCollection 2023"
COMMENT     Sequence Label: myr-palm-Gamillus(2x)
FEATURES             Location/Qualifiers
     source          1..5496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        172..475
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        476..679
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     630..650
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      762..771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             768..1484
                     /codon_start=1
                     /product="acid-tolerant monomeric green fluorescent protein
                     (Shinoda et al., 2018)"
                     /label=Gamillus
                     /translation="MVSKGEEASGRALFQYPMTSKIELNGEINGKKFKVAGEGFTPSSG
                     RFNMHAYCTTGDLPMSWVVIASPLQYGFHMFAHYPEDITHFFQECFPGSYTLDRTLRME
                     GDGTLTTHHEYSLEDGCVTSKTTLNASGFDPKGATMTKSFVKQLPNEVKITPHGPNGIR
                     LTSTVLYLKEDGTIQIGTQDCIVTPVGGRKVTQPKAHFLHTQIIQKKDPNDTRDHIVQT
                     ELAVAGNLWHGMDELYK"
     CDS             1515..2231
                     /codon_start=1
                     /product="acid-tolerant monomeric green fluorescent protein
                     (Shinoda et al., 2018)"
                     /label=Gamillus
                     /translation="MVSKGEEASGRALFQYPMTSKIELNGEINGKKFKVAGEGFTPSSG
                     RFNMHAYCTTGDLPMSWVVIASPLQYGFHMFAHYPEDITHFFQECFPGSYTLDRTLRME
                     GDGTLTTHHEYSLEDGCVTSKTTLNASGFDPKGATMTKSFVKQLPNEVKITPHGPNGIR
                     LTSTVLYLKEDGTIQIGTQDCIVTPVGGRKVTQPKAHFLHTQIIQKKDPNDTRDHIVQT
                     ELAVAGNLWHGMDELYK"
     polyA_signal    2395..2516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2432..2451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2486..2505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2523..2978)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2660..2681)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2872..2891
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3005..3109
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3111..3468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3111..3302
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3134..3154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3319..3454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3503..4297
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3503..4297
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3557..3576)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4167..4186
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4485..4504)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4529..4576
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4905..5493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5394..5413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
       61 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta
      121 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      181 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      241 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      301 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      361 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      421 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      481 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      541 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      601 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      661 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcatg ggatgtatta
      721 aatcaaaaag gaaagacaat ctcaatgacg atgaaccggt cgccaccatg gtgagcaagg
      781 gcgaggaggc atctggcaga gccctgttcc agtaccccat gaccagcaag atcgagctga
      841 acggcgagat caacggcaag aaattcaagg tggccggcga gggcttcacc cccagcagcg
      901 gcagattcaa catgcacgcc tactgcacca ccggcgacct gcctatgagc tgggtcgtga
      961 ttgccagccc cctccagtac ggcttccaca tgttcgccca ctaccccgag gacatcacac
     1021 actttttcca ggaatgcttc cccggcagct acaccctgga ccggaccctg agaatggaag
     1081 gcgacggcac cctgaccacc caccacgagt acagcctgga ggacggctgc gtgacctcca
     1141 agaccaccct gaatgccagc ggcttcgacc ctaagggcgc caccatgacc aagagcttcg
     1201 tgaaacaact gcctaacgag gtgaagatca ccccccacgg ccccaacggc atcagactga
     1261 ccagcaccgt gctgtacctg aaggaggatg gcaccatcca gatcggcacc caggactgca
     1321 tcgtgacccc tgtgggcgga aggaaagtga cccagcccaa ggcccacttc ctgcacaccc
     1381 agatcatcca gaagaaggac cccaacgaca cccgggacca catcgtgcag acagaactgg
     1441 ccgtggccgg caatctgtgg cacggcatgg acgagctgta caagagatct ccgcctgccg
     1501 ctgcaccacc ggtcatggtg agcaagggcg aggaggcatc tggcagagcc ctgttccagt
     1561 accccatgac cagcaagatc gagctgaacg gcgagatcaa cggcaagaaa ttcaaggtgg
     1621 ccggcgaggg cttcaccccc agcagcggca gattcaacat gcacgcctac tgcaccaccg
     1681 gcgacctgcc tatgagctgg gtcgtgattg ccagccccct ccagtacggc ttccacatgt
     1741 tcgcccacta ccccgaggac atcacacact ttttccagga atgcttcccc ggcagctaca
     1801 ccctggaccg gaccctgaga atggaaggcg acggcaccct gaccacccac cacgagtaca
     1861 gcctggagga cggctgcgtg acctccaaga ccaccctgaa tgccagcggc ttcgacccta
     1921 agggcgccac catgaccaag agcttcgtga aacaactgcc taacgaggtg aagatcaccc
     1981 cccacggccc caacggcatc agactgacca gcaccgtgct gtacctgaag gaggatggca
     2041 ccatccagat cggcacccag gactgcatcg tgacccctgt gggcggaagg aaagtgaccc
     2101 agcccaaggc ccacttcctg cacacccaga tcatccagaa gaaggacccc aacgacaccc
     2161 gggaccacat cgtgcagaca gaactggccg tggccggcaa tctgtggcac ggcatggacg
     2221 agctgtacaa gtgagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
     2281 agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
     2341 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
     2401 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2461 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc
     2521 gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2581 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2641 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     2701 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     2761 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     2821 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     2881 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     2941 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa
     3001 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     3061 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga
     3121 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     3181 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     3241 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     3301 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     3361 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     3421 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga
     3481 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
     3541 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
     3601 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
     3661 gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac
     3721 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
     3781 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
     3841 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
     3901 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
     3961 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
     4021 gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
     4081 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
     4141 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
     4201 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
     4261 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
     4321 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
     4381 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     4441 gatctcatgc tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag
     4501 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt
     4561 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc
     4621 ccaccgagac cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc
     4681 ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata
     4741 gcctcaggtt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     4801 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     4861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5041 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5461 tgatgctcgt caggggggcg gagcctatgg aaaaac
//
