LOCUS       sequence-41422-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V207 pRETRO-Triple-Flag SD - Acceptor
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..120
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(504..537)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttcgccct cactccttct ctaggcgccg gaattagatc tctcgagcca catggactac
       61 aaggaccacg acggcgacta caaggaccac gacatcgact acaaggacga cgacgacaag
      121 gtaagtagat cttgacctct caaaagcggg catgacttct gcgctaagat tgtcagtttc
      181 caaaaacgag gaggatttga tattcacctg gcccgcggtg atgcctttga gggtggccgc
      241 atccatctgg tcagaaaaga caatcttttt gttgtcaagc ttggtggcaa acgacccgta
      301 gagggcgttg gacagcaact tggcgatgga gcgcagggtt tggtttttgt cgcgatcggc
      361 gcgctccttg gccgcgatgt ttagctgcac gtattcgcgc gcaacgcacc gccattcggg
      421 aaagacggtg gtgcgctcgt cgggcaccag gtgcacgcgc caacagcggt tgtgcagggt
      481 gacaaggtca acgctaagct tcgataactt cgtatagcat acattatacg aagttataga
      541 tccaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
      601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgcca
//
