LOCUS       sequence-41427-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4440
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..39
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     24..40
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..126)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..126)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(183..202)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(184..202)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(226..243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(477..496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
       61 ctgcagcccg ggggatccac gcgtcacgtg gctagccatg gaaccggtgg atccactagt
      121 tctagagcgg ccgccaccgc ggtggagctc gaattcatcg atgatatcag atctgccggt
      181 ctccctatag tgagtcgtat taatttcgat aagccaggtt gcttcctcgc tcactgactc
      241 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
      301 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
      361 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      421 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      481 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      541 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      601 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
      661 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
      721 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
      781 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
      841 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
      901 ttgatccggc aaacaaacca ccgctggtag cggtggtttt
//
