LOCUS       sequence-41432-e         363 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YAP/TAZ-responsive synthetic promoter driving luciferase expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Dupont S, Morsut L, Aragona M, Enzo E, Giulitti S, Cordenonsi M, 
            Zanconato F, Le Digabel J, Forcato M, Bicciato S, Elvassore N, 
            Piccolo S
  TITLE     Role of YAP/TAZ in mechanotransduction.
  JOURNAL   Nature. 2011 Jun 8;474(7350):179-83. doi: 10.1038/nature10137.
  PUBMED    21654799
REFERENCE   2  (bases 1 to 363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2011-06-8- 8"; volume: "474"; issue: "7350"; pages:
            "179-83"
COMMENT     Sequence Label: 8xGTIIC-luciferase
FEATURES             Location/Qualifiers
     source          1..363
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    join(50..56,57..58,59..65)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(82..88,89..90,91..97)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(129..135,136..137,138..144)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     protein_bind    join(161..167,168..169,170..176)
                     /label=2xGTIIC
                     /bound_moiety="TEF-1 family (TEAD) proteins"
                     /note="dimerized GTIIC motif from the SV40 enhancer, with 
                     high affinity for TEF-1 family (TEAD) proteins (Mahoney et 
                     al., 2005)"
     primer_bind     202..218
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     203..219
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      354..363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg ccagtgccaa gttgagacac attccacaca
       61 ttccactgca agcttgagac acattccaca cattccactg caagcttggc cagtgccaag
      121 ttgagacaca ttccacacat tccactgcaa gcttgagaca cattccacac attccactgc
      181 aagcttctag agatctgcag gtcgaggtcg acggtatcga taagcttggg ggtgggcgcc
      241 ggggggacct taaagcctct gccccccaag gagcccttcc cagacagccg ccggcaccca
      301 ccgctccgtg ggacgatccc ccaagagctt ggcattccgg tactgttggt aaagccacca
      361 tgg
//
