LOCUS       sequence-414380-        4265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  J23107.SoxS-GID1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Alba Burbano D, Cardiff RAL, Tickman BI, Kiattisewee C, Maranas CJ, 
            Zalatan JG, Carothers JM
  TITLE     Engineering activatable promoters for scalable and multi-input 
            CRISPRa/i circuits.
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Jul 25;120(30):e2220358120. doi: 
            10.1073/pnas.2220358120. Epub 2023 Jul 18.
  PUBMED    37463216
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2023-07-25"; volume: "120(30):e2220358120. 
            doi"; pages: " 10.1073/pnas.2220358120. Epub 2023 Jul 18"
COMMENT     Sequence Label: pRC043
FEATURES             Location/Qualifiers
     source          1..4265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(185..289)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     357..375
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             2156..2167
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(2177..2211)
                     /label=BBa_J23107
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.36"
     primer_bind     complement(2659..2676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2830..3418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2910..2929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3589..4265,1..115,116..184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4212..4231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
       61 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      121 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      181 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      241 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      301 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      361 ggcgtatcac gaggcccttt cgtcttcaag aattctggcg aatcctctga ccagccagaa
      421 aacgaccttt ctgtggtgaa accggatgct gcaattcaga gcgccagcaa gtgggggaca
      481 gcagaagacc tgaccgccgc agagtggatg tttgacatgg tgaagactat cgcaccatca
      541 gccagaaaac cgaattttgc tgggtgggct aacgatatcc gcctgatgcg tgaacgtgac
      601 ggacgtaacc accgcgacat gtgtgtgctg ttccgctggg catgccagga caacttctgg
      661 tccggtaacg tgctgagccc ggccaagctt agcccgaaaa gtgccacctg tggcaattcc
      721 gacgtcgatt tcctgcccgg ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgtcagc
      781 attccgcatt aacgaaagcg gagatctcat ccatcacatt atggaagtga ttgttatttg
      841 gtaacagata aaagccaacc gtagctttct ctaagtgcat cagctttact tcttgaccag
      901 ccttcttaag gccttcagca tacgcaagtt gccaatcacg gatcagatcc aggccagcta
      961 ctacgactaa agattttggg aaactgaccc cctccagcga ttttccgcga ggtgaaaagg
     1021 ggttgcatgc ggggtgctcg cggtcctctc cctcgggcaa gaacgctttc caataccagt
     1081 cacgatcgcg aacagtcacg aaatacttcc cgtccaagct tttctcggat tctgtgcgct
     1141 cgttcccccc aaacatcggg ttcagtaaaa tgttgcccag aacatcgatg ccagactctc
     1201 ctgcacgcaa tgcgacatta tgagcgatgt tccctcctga agaatcgccc gctaaaaaaa
     1261 tgtgaacctt cgaatctttc ttgcttttca gccagctacg tgaattaacc caatttaacg
     1321 caatccatcc gtcgtcgtag gcacatgggt aggggttctc cggagcgcga cggtaattca
     1381 cggaaacgac cacgcatttg caaagaccga caagacgacg acataaagtg tcgtagatcg
     1441 cactgttggc ggacgagtgg gcgaacgacc ccccgtggaa aaaaaggatg acagggacaa
     1501 tgtccccatc cactggtttt tccagatcca gaatggatgg aggttgctcc tgatcggcgt
     1561 aagcaggacg atagacacgg gacagaaggt tgatacggcg atcaatcagc acgtcaaaac
     1621 tgaaaacacc gtcaactgga ttagcatttg ctgtaacttt gcggtccaga tattctgcaa
     1681 ggtgacggtt aaaggttcca tccgggcggc gtaagatatt gtacgcgacc ttgaaattcg
     1741 agatcaacac ccaagtattc aagggaacga cggtacgact ctcaataaga tttacctcgt
     1801 cggaggctgc catgctacct ccgccaccca ggcggtggcg ataatccgcg ggagtgcgat
     1861 caaactgccg cgcgaaaacg cgggagaagg tctgctgcga gacataaccc aggtccattg
     1921 cgatatcaaa aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacaggcggc
     1981 gttggcgaat gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatt cgttgcaagt
     2041 accactttga atagcctgat ttttttgcga ctacatcaat gttaagcggc tggtcaatat
     2101 gctcgtcaat ccatgcgata agatcctgaa taattttctg atgggacatg gtacctttct
     2161 cctctttaat gaattcgcta gcataatacc tagggctgag ctagccgtaa aatgcatcct
     2221 agggatatat tccggatcct tactccgtcg acaagcttgc ggccgcactc gaggaattcc
     2281 gactcaatta gttcagtcag tttcaggata ttagtcatct ctacattgat tatgagtatt
     2341 cagaaattcc ttaaatattc tgacaaatgc tctttcccta aactcccccc ataaaaaaac
     2401 ccgccgaagc ggggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc
     2461 ccagacctgc gttcagcagt tctgccaggc tggcagatgc gtcttccgga attgatccgt
     2521 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     2581 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     2641 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     2701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     3001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     3061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     3121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     3181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     3241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     3301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     3361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     3421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     3481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     3541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     3601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     3661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     4021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     4081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     4141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     4201 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     4261 acgtt
//
