LOCUS       sequence-414381-        9388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pET-6xHis-MBP-TEV-BabTIR-APAZ/BabAgo - Heterologous BabSPARTA 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Finocchio G, Koopal B, Potocnik A, Heijstek C, Westphal AH, Jinek M,
            Swarts DC
  TITLE     Target DNA-dependent activation mechanism of the prokaryotic immune 
            system SPARTA.
  JOURNAL   Nucleic Acids Res. 2024 Feb 28;52(4):2012-2029. doi: 
            10.1093/nar/gkad1248.
  PUBMED    38224450
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2024-02-28- 28"; volume: "52"; issue: 
            "4"; pages: "2012-2029"
COMMENT     Sequence Label: pAP007
FEATURES             Location/Qualifiers
     source          1..9388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(278..1360)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1322..1341
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1361..1438)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1644..1663
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1747..1766
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1747..1765
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1766..1790
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1805..1827
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1846..1863
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1873..2973
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     2947..2970
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             2986..3006
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             5998..6015
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6068..6086)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6082..6129
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6166..6621
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6253..6272)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6463..6484
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6714..7529)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6714..7529)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7437..7456
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7521..7540
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7651..8239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8140..8159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8393..8410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8425..8564
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8490..8509)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8650..8672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8666..8857)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
       61 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
      121 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
      181 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
      241 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
      301 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
      361 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
      421 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
      481 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
      541 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
      601 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg ataccctcat
      661 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
      721 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
      781 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
      841 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
      901 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
      961 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     1021 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     1081 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     1141 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     1201 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     1261 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     1321 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     1381 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     1441 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     1501 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     1561 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     1621 tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct
     1681 gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg
     1741 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
     1801 taattttgtt taactttaag aaggagatat accatgggtt cttctcacca tcaccatcac
     1861 catggttctt ctatgaaaat cgaagaaggt aaactggtaa tctggattaa cggcgataaa
     1921 ggctataacg gtctcgctga agtcggtaag aaattcgaga aagataccgg aattaaagtc
     1981 accgttgagc atccggataa actggaagag aaattcccac aggttgcggc aactggcgat
     2041 ggccctgaca ttatcttctg ggcacacgac cgctttggtg gctacgctca atctggcctg
     2101 ttggctgaaa tcaccccgga caaagcgttc caggacaagc tgtatccgtt tacctgggat
     2161 gccgtacgtt acaacggcaa gctgattgct tacccgatcg ctgttgaagc gttatcgctg
     2221 atttataaca aagatctgct gccgaacccg ccaaaaacct gggaagagat cccggcgctg
     2281 gataaagaac tgaaagcgaa aggtaagagc gcgctgatgt tcaacctgca agaaccgtac
     2341 ttcacctggc cgctgattgc tgctgacggg ggttatgcgt tcaagtatga aaacggcaag
     2401 tacgacatta aagacgtggg cgtggataac gctggcgcga aagcgggtct gaccttcctg
     2461 gttgacctga ttaaaaacaa acacatgaat gcagacaccg attactccat cgcagaagct
     2521 gcctttaata aaggcgaaac agcgatgacc atcaacggcc cgtgggcatg gtccaacatc
     2581 gacaccagca aagtgaatta tggtgtaacg gtactgccga ccttcaaggg tcaaccatcc
     2641 aaaccgttcg ttggcgtgct gagcgcaggt attaacgccg ccagtccgaa caaagagctg
     2701 gcaaaagagt tcctcgaaaa ctatctgctg actgatgaag gtctggaagc ggttaataaa
     2761 gacaaaccgc tgggtgccgt agcgctgaag tcttacgagg aagagttggc gaaagatcca
     2821 cgtattgccg ccactatgga aaacgcccag aaaggtgaaa tcatgccgaa catcccgcag
     2881 atgtccgctt tctggtatgc cgtgcgtact gcggtgatca acgccgccag cggtcgtcag
     2941 actgtcgatg aagccctgaa agacgcgcag actaatggga tcgaggaaaa cctgtacttc
     3001 caatccaatg caagaaacaa aatcttcata agccatgccg cacctgatga caacgatttt
     3061 acaaaatggt tggcattaaa gctcattgcg ctgggttatg aagtatggtg tgatgttctg
     3121 ttcttggata aaggagctga tttttggaaa gtaatagata aggaaatccg tgaaggggca
     3181 attaagttct tgctggcaac ttcagaaata gctattaagc gagatggtgt gctaaaagaa
     3241 attgccgttg cggaaaaagt gaaaaagcaa ctgaaggatg ataatttcat cattccctta
     3301 atcattgacg aaaatctttc ttatgacgat ttgccgcccg aaattattcg ccttaatgcg
     3361 gtagacttca aaaagtcttg ggctgtaggt ttgcaagatt tattgaaagc acttgatgac
     3421 caaaaagtag aaaagaacag tccagacccc gacaagagca atgccttgta tcaacagatt
     3481 tttctgcaca ataaaggaat aattgaacga gaagaaattt atgattccaa ttggttcagc
     3541 attttatcat ttcccaaaga attgcggttt catgattatg aaaagttgat gccaaaagga
     3601 tttgatgtcc gtgagcttac ttatcctgcc gttcgctaca aaaactatct ctgcacgttt
     3661 gcttgggaat atgatttcat gcatcagtta ccaaaaacag aaacatacaa cagcagccaa
     3721 acaattagaa taccaactga agaaatacta tcgggcaaat acgattcgcc attcattggc
     3781 aactttgaat gtcagcggtt gattgttcaa ctattgaata aggcgtttga attgagaatg
     3841 aaagagaaag gagtgcggga atatcctatg tcaaacaaaa tgggttattg gtttgaaaag
     3901 ggaaagctgg agaaggataa gttcaataaa gtgctgttgg ttggtaagca aaaagataaa
     3961 cattggcact tcggtatatc agcagctggt aaactctatc cgtttcctgt gttgatgatt
     4021 tcgtcacata tctttttcac caaagatggt aaagaactca ttgaaagcaa aaaaatacaa
     4081 catgcagcac gaagaagaca gggtaaaaat tggtggaatg acgattggcg aaataaacta
     4141 cttgccttcg taaaatactt gtcagatgat gaaaacagtt tttatttgga agtagggagt
     4201 gaagagaaaa tttatatctc aaacgaacct gttcagtttg tgggaaaagt gagctacaat
     4261 atgcctgaga aaaataacct gaaagatgaa gcggaaattt cagatttaaa tgatttgaat
     4321 gaatttgatg gagaaatttt tgaagaaacc gactctgaat gactcgaagc ttagatttta
     4381 aataacaata ctaaggagga attacatatg aaggaattga tttacataca tgaaccgaat
     4441 atactttttg caaatggaca aaaatgtgca gacccaagag acggactagc tttgtttggc
     4501 ccattcacaa agatatatgg cattaaaagc ggtgtagttg gaactcagta cggtttaagc
     4561 atcttcaaaa attacataaa tcacatacaa aagcccatct ataacgctaa taacattacc
     4621 cgacccatgt ttcctggatt tgaagccgta tttggttgca aatgggatgc agataatgtt
     4681 gttttcaaag aagtgaccaa agaagaaata gagaaaattt tatataccga aagcaaccat
     4741 aaaaggacgt atgatttggt atcattgttt attaataaaa taattacagc aaacaagaat
     4801 gaagatgaaa aggtagatgt ttggtttcta gttattcctg atgaaatcta tcaatattgc
     4861 cgacctaatt cggttttacc aaaagatttg gttcaaacga aatcattaat cactaaatca
     4921 aaggcgaaat catttcggta tgaaccaacc ttgtttgaaa acataaacaa ggaattaaag
     4981 gagcaggaaa aagaagcaat tacttacaac tatgacgcac agttccatga ccaattaaaa
     5041 gcccgattgc ttgaacacac aatccccaca caaatactaa gagagagcac cttggcatgg
     5101 cgagatttca agaacaagtt tggtgcaccc aaaagagatt tctcaaaaat tgaaggacat
     5161 ttagcttgga caatttcaac agctgcattt tacaaagctg gtggtaaacc ttggaaacta
     5221 tcagatatta gaagcggagt atgctatctc gggcttgttt acaagcaaat tgaaaaaagt
     5281 agtaatccaa agaatgcttg ttgtgcagca caaatgtttt tggataatgg tgacggcaca
     5341 gtattcaaag gagaagttgg gccgtggtat aatcaagaaa aacacgaatt tcatttaaat
     5401 ccgaaagaag caaaagcatt attaactcag gcattaaatt catacaaaga acaaaatggt
     5461 gtttttccca aagagatttt tattcatgcc aagacaaagt ttaacggaca ggaatggaat
     5521 gcattccaag aagtaacacc ggaaggaact aatttggtgg gagtaacaat aactaagaca
     5581 aagcccttaa aactgtttaa atcagaagga aactatccga taatgagagg taacgccttt
     5641 atcgtaaatg aaaggagtgc attcctttgg acagtgggct atgttccaaa gacagaatca
     5701 actttgtcaa tggaagttcc caatccaatt ttcattgaaa tcaataaagg cgaagctgac
     5761 attgagcagg ttttgaaaga tgttttagca ttaactaaat tgaattacaa tgcttgtatt
     5821 tatgcagacg gagttcctgt aacacttcgc tttgcagaca aaataggaga aatattaacc
     5881 gccagcacag aactaaaagc accaccactt gcattcaaat actacattta ataacattgg
     5941 aagtggataa cggatccgaa ttcgagcgcc gtcgacaagc ttgcggccgc actcgagcac
     6001 caccaccacc accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     6061 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     6121 ggttttttgc tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg
     6181 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6241 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6301 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6361 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6421 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6481 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6541 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     6601 aactagtaac gtttacaatt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6661 tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa
     6721 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     6781 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     6841 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     6901 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     6961 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     7021 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     7081 gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     7141 cggcgcagga acactgccag cgcatcaaca atgttttcac ctgaatcagg atattcttct
     7201 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     7261 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     7321 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     7381 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     7441 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     7501 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     7561 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8221 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8401 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     8461 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8521 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     8581 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     8641 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     8701 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     8761 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     8821 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     8881 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     8941 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     9001 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
     9061 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
     9121 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg
     9181 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
     9241 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
     9301 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc
     9361 cgaaacgttt ggtggcggga ccagtgac
//
