LOCUS       sequence-414387-       10833 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FR-GECO1c and CoChR in the zebrafish heart.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   Jia BZ, Qi Y, Wong-Campos JD, Megason SG, Cohen AE
  TITLE     A bioelectrical phase transition patterns the first vertebrate 
            heartbeats.
  JOURNAL   Nature. 2023 Oct;622(7981):149-155. doi: 10.1038/s41586-023-06561-z.
            Epub 2023 Sep 27.
  PUBMED    37758945
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06561-z"; journalName: "Nature"; date: 
            "2023-10"; volume: "622"; issue: "7981"; pages: "149-155"
COMMENT     Sequence Label: pBJ084_zACNE20_myl7_FR-GECO1c_P2A_CoChR_KGC_eG...
FEATURES             Location/Qualifiers
     source          1..10833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(411..435)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(814..976,977..988)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2838..2857)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2839..2857)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2867..2884)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2867..2883)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2876..2898)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3025..3480
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3112..3131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3322..3343
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3506..3610
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3611..3679,3680..4471)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3829..3848)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4642..5230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5131..5150
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5384..5401
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5518..5539
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5554..5559,5560..5577,5578..5584)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5592..5608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5597..5619
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5616..5632
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5616..5632
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5651..5671
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5653..5671
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     5708..5724
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5708..5724
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(5758..5769,5770..5957)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    6394..6528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6431..6450)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6485..6504
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(6577..7287,7288..7290))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(7399..8259)
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="LGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFSILI
                     LMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEWL
                     LTCPVILIHLSNLTGLKDDYSKRTMRLLVSDVGTIVWGATSAMSTGYVKVIFFVLGCIY
                     GANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISVY
                     GSTIGHTIIDLMSKNCWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVDQ
                     EDEETV"
     CDS             complement(8260..8316)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    10477..10501
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccgacagcgg ctccacagca ggcctgagcg ccaccaccca ccatcccaaa aaaatactga
       61 taacacaaat cagcgagtgt gtgagacaca ggagttccca tttcgcttat agtttccaca
      121 acaatgttaa gcagcaaacc gaaaagctga catattgggg tgatttctga aggatcatgt
      181 ggcactgaag actagagtaa tggtgctaaa aatgcagatt tgcctgaata gcttaaaaac
      241 tggcaatagt ttaaggacaa atcctcactt ttaccagcct attattaaga tattggctga
      301 ttattggtag ttatgaagca catattctgc aagatcttac tctacatcca taatcctctt
      361 caataccgaa acttaaccta cgtatttaac atttacattc tcactctgcg agcctgcttt
      421 tttgtacaaa cttgtgatat cctcgagggc ccatctggcc tgtgtttcag acaccaggga
      481 gtctctgctc acgtttcctg ctatttgcag cctctctatc aagactaata cacctcttcc
      541 cgcatcggct gcctgtgaga ggcttttcag cactgcagga ttgcttttca gccccaaaag
      601 agctaggctt gacactaaca attttgagaa tcagcttcta ctgaagttaa atctgaggtt
      661 ttacaacttt gagtagcgtg tactggcatt agattgtctg tcttatagtt tgataattaa
      721 atacaaacag ttctaaagca ggataaaacc ttgtatgcat ttcatttaat gttttttgag
      781 attaaaagct taaacaagaa tctctagttt tctttcttgc ttttactttt acttccttaa
      841 tactcaagta caattttaat ggagtacttt tttactttta ctcaagtaag attctagcca
      901 gatactttta cttttaattg agtaaaattt tccctaagta cttgtacttt cacttgagta
      961 aaatttttga gtacttttta cacctctgtc aagaactcct ggacaaacct ctgacctgtg
     1021 tggaacagag tggatatggg tgtctgaaca gatattcacg tcttttgcag atcagagggc
     1081 atttctggtg ttgtgcaatg acctgggtcc aacaggtagg cgtactcgta tccaagagca
     1141 ttgttggaca ggaggtagtc tccattccta taaagaggaa ggaatggcac catgtagtag
     1201 ccgtcattgt ggccaattgg tgcattggta cgtgggtaga tggaccgggg aggctgatga
     1261 gttcttagcc agcgctcaaa gatgctagaa atgatgtaag aaacaaatat gcaagatgaa
     1321 agttaagata agataaaaaa ttaaacaaat aaatgcaatt atgttttgtg tttctattcc
     1381 accaataata acctcacgca gtaaagccac cagattattt tttgttcttt gctctgcagg
     1441 gcaaccaaac tctaacaatt gatctcaatt gatctttcgc tgctcatcca agtacattca
     1501 caaacttcac taattgtagc agctataaac aacagattgt atgaactttg tcaaaaactg
     1561 tgcagtctaa tgtcatgacg tgtttgttac ctgtcaatga aagcatggtg aaggaggaag
     1621 atggggtcgt tggctgagcc ctggactgag gacattgagc cgttcatgaa gacatgcagc
     1681 gcattgtgca tggtgctctg gccttgcaca gccatgcctg tttctgggct ggcaaaacct
     1741 acaaagaaaa gaagcaggtg aagaagttac agtttcaatc aagataacag caaaacaagg
     1801 ctaattaaaa gacagcaaaa aaaggagatg taaaatatag ctattgattt tctttacttt
     1861 tctttacgta ggcaaatgtg acttaagtgt gttgttgtat cccccccccc cccagttttt
     1921 tatgtatgca tttaatcatt tctgtaatga atctgtggaa attatttctt attttgacta
     1981 ccaatcaatc aatcaatcaa tcaatcaatc aatcaatcaa tcaatcaatc aatcaatcaa
     2041 tcaatcaatc aatcaatcta ccaaccaacc aaccaaccaa cgactgtata tgagaactgt
     2101 aatgagtgac ctcctccttc tagtgatcca aacaggaaga agctccaaat ggcatgaatg
     2161 ctccagtggt ggcattcata ttaaaatctt tatttaacat gtacttgtta atcataattt
     2221 ctgtaataac ttttcttttc tgtagcccaa gttgttgata ttataaactg accaaccaga
     2281 tgccttaata aaagtatgtg ctctcacatc caacgttcaa aacgtttgac agattttgtg
     2341 cagcccgctt tcaagtggta ggctgtggac ttccaacaag cttacttctg attggttaga
     2401 ggggttgcca taggaacggt gactcagact cgacgcatct cggctctttc ttgcttattg
     2461 gcaacgtcta gctccaacac ggcgatgtcc atacagtgaa aaatggcaac tgaattaact
     2521 tcattttgtt ggagccagaa gtaagtcatt ttgaatgggt gacgtaacac tcactcggtc
     2581 cagttctcaa atacagtcaa caagtactat tacataataa tagtgtgtac tactattaca
     2641 tattattgta ataatgtctg ttaaacacag tcataattga aaaaaaacaa ctaaaacatt
     2701 tattcaaggg taaagacaaa tgcagacaaa tacagaaaag atttaaaata gcagtagaaa
     2761 atggcaaacg ggcttattca tccaagtttt cagcacttgt acaggtgggt aattcctact
     2821 atttggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     2881 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2941 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     3001 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3061 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3121 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3181 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3241 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3301 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3361 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3421 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3481 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3541 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3601 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3661 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3721 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3781 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     3841 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     3901 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     3961 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4021 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4081 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4141 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4201 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4261 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4321 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4381 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4441 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4501 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4561 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4621 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4681 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4741 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4801 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     4861 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     4921 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4981 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5041 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5101 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5161 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5221 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5281 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     5341 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     5401 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     5461 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     5521 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     5581 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     5641 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg
     5701 cggccgctct agaactagtg gatctgctgg gcttgctgaa ggtagggggt caagaaccag
     5761 aggtgtaaag tacttgagta attttacttg attactgtac ttaagtatta tttttgggga
     5821 tttttacttt acttgagtac aattaaaaat caatactttt acttttactt aattacattt
     5881 ttttagaaaa aaaagtactt tttactcctt acaattttat ttacagtcaa aaagtactta
     5941 ttttttggag atcacttcat tctattttcc cttgctatta ccaaaccaat tgaattgcgc
     6001 tgatgcccag tttaatttaa atgttattta ttctgcctat gaaaatcgtt ttcacattat
     6061 atgaaattgg tcagacatgt tcattggtcc tttggaagtg acgtcatgtc acatctatta
     6121 ccacaatgca cagcaccttg acctggaaat tagggaaatt ataacagtca atcagtggaa
     6181 gaaaatggag gaagtatgtg attcatcagc agctgcgagc agcacagtcc aaaatcagcc
     6241 acaggatcaa gagcacccgt ggccgtatct tcggatctga tctagaggat cataatcagc
     6301 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     6361 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     6421 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6481 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcat catcgatgaa
     6541 ttaattcgag cttcacacct cgttctcgta acagaacttg tacagctcgt ccattcccag
     6601 tgtgattcca gcagctgtca cgaactccag cagcaccatg tggtctctct tctcgttagg
     6661 gtccttgctc agagcgctct gtgtgctcag gtagtggttg tcaggcagca gcacaggtcc
     6721 gtctccgata ggtgtgttct gctggtagtg gtcagccagc tgcacgcttc cgtcctcgat
     6781 gttgtgtctg atcttgaagt tcaccttgat tccgttcttc tgcttgtcag ccatgatgta
     6841 cacgttgtgg ctgttgtagt tgtactccag cttgtgtccc aggatgtttc cgtcctcctt
     6901 gaagtcgatt cccttcagct cgattctgtt caccagtgtg tctccctcga acttcacctc
     6961 agctcttgtc ttgtagtttc cgtcgtcctt gaagaagatt gttctctcct gcacgtatcc
     7021 ctcaggcata gcgctcttga agaagtcgtg ctgcttcatg tggtcagggt atctgctgaa
     7081 acactgcact ccgtatgtca gtgttgtcac cagtgtaggc caaggcacag gcagctttcc
     7141 tgttgtacag atgaacttca gtgtcagctt tccgtatgta gcgtctccct ctccctctcc
     7201 gctcacgctg aacttgtgtc cgttcacgtc tccgtccagc tccaccagga taggcaccac
     7261 tcctgtgaac agctcctctc ccttgctcac cacgttgatg tcgatctggt ccagagggat
     7321 gtactctccc tcgcttgtga ttctgctctt agcagcagcc ttgctcacag ccaccacagg
     7381 tctgtcagca gcagatctca ctgtctcctc gtcctcctgg tccaccatgg tctccacttc
     7441 catctcctcg ccggccacgt tgatcttggt cttcttccgg atgtcgccgt agatgatgat
     7501 gtgctggtgg atcagcactc tcagatagtg tcccagcagg ccccaacaat tcttgctcat
     7561 gaggtcgatg atggtgtggc caatggtaga gccgtacacg ctgatggcgt cgaatccttc
     7621 tggtcccacg acgaacagca cggggaacat gccccaagac aggaagaaca gccaagccat
     7681 gatccgcacg acggttctag gtctgccctt tggcaccacg tggtagctct cgatatacac
     7741 cttggcggcg tggaagaagg tgttggcgcc gtagatgcaa cccagcacga agaagatgac
     7801 cttgacgtag cctgtgctca tggcgcttgt agctccccac acgatggttc ccacgtctga
     7861 caccagcagc ctcatggtcc gcttgctgta gtcgtccttc aggccggtca ggttgctcag
     7921 gtggatcagg atgacgggac aagtcagcag ccactctgcg tatctcagcc actggactcg
     7981 gtgtccgtta gccaggtaca gcatgctggg gtcgtcgaac tcgtggaaga actcgatgat
     8041 gaccttggtc agctcgacgc aacagacgta gacctcctcc caaccgcaag tggctctcca
     8101 agtctggtag gcgtagaaca tcaggatcag gatgctgaag ccgaaggcga accactggag
     8161 gatgttggcc accagctgct ctgtatcgct tcccaggctg tcggtccaag ccaggcaaaa
     8221 gcactggtcg ataggcacaa tggcgctgcc gtttcccagg ggaccggggt tttcttctac
     8281 gtctcctgct tgctttaaca gagagaagtt cgtggctccg gagcccttgg cggtcatcat
     8341 ctgcacgaac tcttcgtagt tcacttgtcc gtcgccgtcg atgtcggcca ctctgatcat
     8401 ctcgtcaacc tcttcgtcag tcagcttctc gcccagatcg gccatcacgc gtctcagttc
     8461 ggcggctccg atgtagccgt tgccatcctt atcgaaaacc tggaaggctt cccggatctc
     8521 ttcctcggtc actctgtagc tcatcttccg ggacatcatg gtcaggaact cagggtaatc
     8581 gaaggttccg tcgccatcgg cgtccacctc gttgatcatg tcctggagtt cggcttctgt
     8641 tggattctgg cccaggctcc gcatcacgga gcccagttct ttggtggtga tggtgccgtc
     8701 gccgtccttg tcgaacaggc tgaaggcttc tttgaactcg gcgatcagtt cttctgtcag
     8761 cgtgccatcg gtgggcaccc agccgagggt tttcttctgc atcacgggtc catcgctggg
     8821 gaagttcacg cccagcagct tcacattgta gatcaggtag ccgtcctgga ggcttgtatc
     8881 ctgtgtcacg gtcagcacgc cgccatcctc gtatgtggtc actctctccc aggtgaagcc
     8941 ctcggggaag ctctgcttga agaaatcggg gatgccctgg ggatagttga tgaaggtctt
     9001 gctgccgtac ataaaacagg tggccaggat gtcaaaggcg aaaggcagag ggccaccttc
     9061 caccaccttg atccgctggg tctgtgttcc ctcatagggc ttgccctcgc cttcggcagt
     9121 acacttgaag tggtggttgc cgacggtccc ttccaagtag agcttcatcc gcatgttttc
     9181 tttgatcagc tcagacacag atccgcctgt tcctccgcct ccgccaagtc caacatatct
     9241 ggccacggcc acttcgtgct gttccacgta tgtctcgttg tcggcctctt tgattctttc
     9301 cagcttccgg cccacctggt gttcgccagg catcttcagg ttcttggcgg gcttcttgct
     9361 tctgtaggtg gttttcaggt tgcacttcag gtgtccgccg ccaacgagtc tcagggccat
     9421 gtcacatgtg cctgccaggc caccgtccac aggcaccagg gtctcggtca gaaaaccgaa
     9481 ggggttgccg aagctccgct tccgcagcat ggacttgacc aggatcactg tggtcaggct
     9541 agggatctgc ttcacgctgc ccatggtcac tgtctgcttt gctgttggtc tgggctcctg
     9601 ggtcactggc ttactaatgg agtctttatg tatgaggact cttatcaatt gttcttctat
     9661 aaaggtctgc agtgtttctg ttcgtcccct acatggacac ccagagcctc ctaaatacag
     9721 gagccctgat aactgcacaa gtgctcagat tccagcaggg tggaaatgag ataaagtgtg
     9781 cagatgggga gggggacgtg aatgagagat ttgagggatg aaaaggatgg atgaacgcat
     9841 tgaaaatagc ccctttcaca aaataatacc agtaaattgt catataatta atgaccagac
     9901 ctttacctgt aaatgatgtg atgttcatat acagaagaat gccaacaatt tacagatgat
     9961 tttacaaatt cttaattttc cttttctaaa tggatttgtt cacacattat ctcttcaaag
    10021 caattgaatt ttctggaaaa gattggctgt gtgtgtaaaa ggtgctactg aggaatttaa
    10081 tgtgacatgg aatgaagcaa acagcagcct aagacaggat ggcaggaaaa atgtcacatg
    10141 tttaaaataa gagtgaaacc aacgacctgg acttatagag actgtgtgtc cttaggcaaa
    10201 gctcttgtac aaacagtgct tgtgactgtg aaattcgggg tttgcctgga ttgtgttaaa
    10261 tgtgtgcttg gatgtatcat aaaaaagaat cggttctctg tatttgacat tttctgtaat
    10321 ttatctaaag ttacaactgg ctatgcctga tttttatcac tattgaatca atcattttaa
    10381 agaatgttaa gtctgctgat ttggagagct catttacatt tattttgaat gtctcttatt
    10441 taacacaact gatttaagct ttgagatctt gatatcacca ctttgtacaa gaaagctggg
    10501 tgtggtagaa agtaatacag gtttggattg aagtaagggt ggatacaaga tgactcaatt
    10561 ttggctgaat cttcccttaa atcatcaata catttgccat cctacatgcc taatcaaatc
    10621 cagaggtttt cagagtttgg gaaaactgct gattgcatcc ttatcccatc cttcctcttc
    10681 ctcactgtgc cgtatagctg tgccctgtct atcagttcgg gccatggaag agcgtgtcac
    10741 ccagtcactc aggggctcag ataggcctgg ctccctgcta gtcctgggac gtggcttatc
    10801 aggcaggcct ggctccattt gagggggagt ggg
//
