LOCUS       sequence-414401-       10791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Voltron1 and jGCaMP7f in the zebrafish heart.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Jia BZ, Qi Y, Wong-Campos JD, Megason SG, Cohen AE
  TITLE     A bioelectrical phase transition patterns the first vertebrate 
            heartbeats.
  JOURNAL   Nature. 2023 Oct;622(7981):149-155. doi: 10.1038/s41586-023-06561-z.
            Epub 2023 Sep 27.
  PUBMED    37758945
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-023-06561-z"; journalName: "Nature"; date: 
            "2023-10"; volume: "622"; issue: "7981"; pages: "149-155"
COMMENT     Sequence Label: pBJ089_zACNE20_myl7_Voltron1_P2A_jGCaMP7f
FEATURES             Location/Qualifiers
     source          1..10791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(82..670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(162..181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(841..1632,1633..1701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1464..1483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1702..1806)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1832..2287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1969..1990)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2181..2200
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2455..2474
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2455..2473
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(4324..4335,4336..4498)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    4877..4901
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5644..5668)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8246..8302
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8315..8371
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     8584..8605
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8711..8732)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8972..8991)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    9575..9709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9599..9618)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9653..9672
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(10146..10333,10334..10345)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10379..10395)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10379..10395)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10432..10452)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10432..10450)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10471..10487)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10471..10487)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10484..10506)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10495..10511
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10519..10525,10526..10543,10544..10549))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10564..10585
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10702..10719)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
       61 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      121 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      181 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      241 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      301 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      361 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      421 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
      481 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
      541 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
      601 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
      661 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
      721 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
      781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
      841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
      901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      961 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     1501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     1561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     1621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     1681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     1741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     1801 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     1861 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1921 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1981 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2041 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2101 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2161 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2221 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2281 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     2341 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     2401 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
     2461 gactcactat agggcgaatt gggtaccaaa tagtaggaat tacccacctg tacaagtgct
     2521 gaaaacttgg atgaataagc ccgtttgcca ttttctactg ctattttaaa tcttttctgt
     2581 atttgtctgc atttgtcttt acccttgaat aaatgtttta gttgtttttt ttcaattatg
     2641 actgtgttta acagacatta ttacaataat atgtaatagt agtacacact attattatgt
     2701 aatagtactt gttgactgta tttgagaact ggaccgagtg agtgttacgt cacccattca
     2761 aaatgactta cttctggctc caacaaaatg aagttaattc agttgccatt tttcactgta
     2821 tggacatcgc cgtgttggag ctagacgttg ccaataagca agaaagagcc gagatgcgtc
     2881 gagtctgagt caccgttcct atggcaaccc ctctaaccaa tcagaagtaa gcttgttgga
     2941 agtccacagc ctaccacttg aaagcgggct gcacaaaatc tgtcaaacgt tttgaacgtt
     3001 ggatgtgaga gcacatactt ttattaaggc atctggttgg tcagtttata atatcaacaa
     3061 cttgggctac agaaaagaaa agttattaca gaaattatga ttaacaagta catgttaaat
     3121 aaagatttta atatgaatgc caccactgga gcattcatgc catttggagc ttcttcctgt
     3181 ttggatcact agaaggagga ggtcactcat tacagttctc atatacagtc gttggttggt
     3241 tggttggttg gtagattgat tgattgattg attgattgat tgattgattg attgattgat
     3301 tgattgattg attgattgat tgattgattg gtagtcaaaa taagaaataa tttccacaga
     3361 ttcattacag aaatgattaa atgcatacat aaaaaactgg gggggggggg natacaacaa
     3421 cacacttaag tcacatttgc ctacgtaaag aaaagtaaag aaaatcaata gctatatttt
     3481 acatctcctt tttttgctgt cttttaatta gccttgtttt gctgttatct tgattgaaac
     3541 tgtaacttct tcacctgctt cttttctttg taggttttgc cagcccagaa acaggcatgg
     3601 ctgtgcaagg ccagagcacc atgcacaatg cgctgcatgt cttcatgaac ggctcaatgt
     3661 cctcagtcca gggctcagcc aacgacccca tcttcctcct tcaccatgct ttcattgaca
     3721 ggtaacaaac acgtcatgac attagactgc acagtttttg acaaagttca tacaatctgt
     3781 tgtttatagc tgctacaatt agtgaagttt gtgaatgtac ttggatgagc agcgaaagat
     3841 caattgagat caattgttag agtttggttg ccctgcagag caaagaacaa aaaataatct
     3901 ggtggcttta ctgcgtgagg ttattattgg tggaatagaa acacaaaaca taattgcatt
     3961 tatttgttta attttttatc ttatcttaac tttcatcttg catatttgtt tcttacatca
     4021 tttctagcat ctttgagcgc tggctaagaa ctcatcagcc tccccggtcc atctacccac
     4081 gtaccaatgc accaattggc cacaatgacg gctactacat ggtgccattc cttcctcttt
     4141 ataggaatgg agactacctc ctgtccaaca atgctcttgg atacgagtac gcctacctgt
     4201 tggacccagg tcattgcaca acaccagaaa tgccctctga tctgcaaaag acgtgaatat
     4261 ctgttcagac acccatatcc actctgttcc acacaggtca gaggtttgtc caggagttct
     4321 tgacagaggt gtaaaaagta ctcaaaaatt ttactcaagt gaaagtacaa gtacttaggg
     4381 aaaattttac tcaattaaaa gtaaaagtat ctggctagaa tcttacttga gtaaaagtaa
     4441 aaaagtactc cattaaaatt gtacttgagt attaaggaag taaaagtaaa agcaagaaag
     4501 aaaactagag attcttgttt aagcttttaa tctcaaaaaa cattaaatga aatgcataca
     4561 aggttttatc ctgctttaga actgtttgta tttaattatc aaactataag acagacaatc
     4621 taatgccagt acacgctact caaagttgta aaacctcaga tttaacttca gtagaagctg
     4681 attctcaaaa ttgttagtgt caagcctagc tcttttgggg ctgaaaagca atcctgcagt
     4741 gctgaaaagc ctctcacagg cagccgatgc gggaagaggt gtattagtct tgatagagag
     4801 gctgcaaata gcaggaaacg tgagcagaga ctccctggtg tctgaaacac aggccagatg
     4861 ggccctcgag gatatcacaa gtttgtacaa aaaagcaggc tcgcagagtg agaatgtaaa
     4921 tgttaaatac gtaggttaag tttcggtatt gaagaggatt atggatgtag agtaagatct
     4981 tgcagaatat gtgcttcata actaccaata atcagccaat atcttaataa taggctggta
     5041 aaagtgagga tttgtcctta aactattgcc agtttttaag ctattcaggc aaatctgcat
     5101 ttttagcacc attactctag tcttcagtgc cacatgatcc ttcagaaatc accccaatat
     5161 gtcagctttt cggtttgctg cttaacattg ttgtggaaac tataagcgaa atgggaactc
     5221 ctgtgtctca cacactcgct gatttgtgtt atcagtattt ttttgggatg gtgggtggtg
     5281 gcgctcaggc ctgctgtgga gccgctgtcg gcccactccc cctcaaatgg agccaggcct
     5341 gcctgataag ccacgtccca ggactagcag ggagccaggc ctatctgagc ccctgagtga
     5401 ctgggtgaca cgctcttcca tggcccgaac tgatagacag ggcacagcta tacggcacag
     5461 tgaggaagag gaaggatggg ataaggatgc aatcagcagt tttcccaaac tctgaaaacc
     5521 tctggatttg attaggcatg taggatggca aatgtattga tgatttaagg gaagattcag
     5581 ccaaaattga gtcatcttgt atccaccctt acttcaatcc aaacctgtat tactttctac
     5641 cacacccagc tttcttgtac aaagtggtga tatcaagatc tcaaagctta aatcagttgt
     5701 gttaaataag agacattcaa aataaatgta aatgagctct ccaaatcagc agacttaaca
     5761 ttctttaaaa tgattgattc aatagtgata aaaatcaggc atagccagtt gtaactttag
     5821 ataaattaca gaaaatgtca aatacagaga accgattctt ttttatgata catccaagca
     5881 cacatttaac acaatccagg caaaccccga atttcacagt cacaagcact gtttgtacaa
     5941 gagctttgcc taaggacaca cagtctctat aagtccaggt cgttggtttc actcttattt
     6001 taaacatgtg acatttttcc tgccatcctg tcttaggctg ctgtttgctt cattccatgt
     6061 cacattaaat tcctcagtag caccttttac acacacagcc aatcttttcc agaaaattca
     6121 attgctttga agagataatg tgtgaacaaa tccatttaga aaaggaaaat taagaatttg
     6181 taaaatcatc tgtaaattgt tggcattctt ctgtatatga acatcacatc atttacaggt
     6241 aaaggtctgg tcattaatta tatgacaatt tactggtatt attttgtgaa aggggctatt
     6301 ttcaatgcgt tcatccatcc ttttcatccc tcaaatctct cattcacgtc cccctcccca
     6361 tctgcacact ttatctcatt tccaccctgc tggaatctga gcacttgtgc agttatcagg
     6421 gctcctgtat ttaggaggct ctgggtgtcc atgtagggga cgaacagaaa cactgcagac
     6481 ctttatagaa gaacaattga taagagtcct catacataaa gactccatta gtaagccagt
     6541 gacccaggag cccagaccaa cagcaaagca gacagtgacc atggctgacg tggaaaccga
     6601 gaccggcatg attgcacagt ggattgtctt tgctattatg gctgctgctg ctattgcttt
     6661 tggagtggct gtgcactttc ggccttcaga gctgaagagc gcatactata tcaacattgc
     6721 catctgcact atcgccgcta ccgcttacta tgcaatggcc gtgaactacc aggacctgac
     6781 aatgaatggt gaaaggcagg tggtctacgc aagatatatt aactgggtgc tgaccacacc
     6841 actgctcctg ctcgatctca tcgtcatgac caagatgggc ggagtgatga tttcttgggt
     6901 catcggcgca gacattttca tgatcgtgtt tggtattctg ggcgccttcg aggatgaaca
     6961 caagttcaaa tgggtgtact ttatcgctgg atgtgtgatg caggcagtcc tgacatacgg
     7021 gatgtataac gccacttgga aagacgatct gaagaaaagc cccgagtacc atagctccta
     7081 tgtcagtctg ctcgtcttcc tgtcaatcct ctgggtgttt tatcctgtcg tgtgggcttt
     7141 cgggtctggt agtggcgtgc tgtccgtcga caatgaggcc attctcatgg gaatcctgga
     7201 tgtgctcgct aagccactgt ttggaatggg gtgcctcatt gcccatgaga ctatcttcaa
     7261 gatcggtact ggctttccat tcgaccccca ttatgtggaa gtcctgggcg agcgcatgca
     7321 ctacgtcgat gttggtccgc gcgatggcac ccctgtgctg ttcctgcacg gtaacccgac
     7381 ctcctcctac gtgtggcgca acatcatccc gcatgttgca ccgacccatc gctgcattgc
     7441 tccagacctg atcggtatgg gcaaatccga caaaccagac ctgggttatt tcttcgacga
     7501 ccacgtccgc ttcatggatg ccttcatcga agccctgggt ctggaagagg tcgtcctggt
     7561 cattcacgac tggggctccg ctctgggttt ccactgggcc aagcgcaatc cagagcgcgt
     7621 caaaggtatt gcatttatgg agttcatccg ccctatcccg acctgggacg aatggccaga
     7681 atttgcccgc gagaccttcc aggccttccg caccaccgac gtcggccgca agctgatcat
     7741 cgatcagaac gtttttatcg agggtacgct gccgatgggt gtcgtccgcc cgctgactga
     7801 agtcgagatg gaccattacc gcgagccgtt cctgaatcct gttgaccgcg agccactgtg
     7861 gcgcttccca aacgagctgc caatcgccgg tgagccagcg aacatcgtcg cgctggtcga
     7921 agaatacatg gactggctgc accagtcccc tgtcccgaag ctgctgttct ggggcacccc
     7981 aggcgttctg atcccaccgg ccgaagccgc tcgcctggcc aaaagcctgc ctaactgcaa
     8041 ggctgtggac atcggcccgg gtctgaatct gctgcaagaa gacaacccgg acctgatcgg
     8101 cagcgagatc gcgcgctggc tgtcgacgct cgagatttcc ggcgagccaa ccactaagag
     8161 cagaatcaca agcgagggag agtacatccc tctggaccag atcgacatca acgtgttctg
     8221 ttacgagaac gaggtgggct ccggagccac gaacttctct ctgttaaagc aagcaggaga
     8281 cgtggaagaa aaccccggtc ccgtcgactc atcacgtcgt aagtggaata agacaggtca
     8341 cgcagtcaga gctataggtc ggctgagctc actcgagaac gtctatatca aggccgacaa
     8401 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcggcgt
     8461 gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     8521 cgacaaccac tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga
     8581 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     8641 gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt tcaccggggt
     8701 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     8761 cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     8821 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     8881 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     8941 ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     9001 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag cttaagggca tcgacttcaa
     9061 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ctgccggacc aactgactga
     9121 agagcagatc gcagaattta aagagctgtt ctccctattt gacaaggacg gggatgggac
     9181 aataacaacc aaggagctgg ggacggtgat gcggtctctg gggcagaacc ccacagaagc
     9241 agagctgcag gacatgatca atgaagtaga tgccgacggt gacggcacaa tcgacttccc
     9301 tgagttcctg acaatgatgg caagaaaaat gaaatacaca gacagtgaag aagaaattag
     9361 agaagcgttc cgtgtgtttg ataaggatgg caatggctac atcagtgcag cagagcttcg
     9421 ccacgtgatg acaaaccttg gagagaagtt aacagatgaa gaggttgatg aaatgatcag
     9481 ggaagcagac atcgatgggg atggtcagtt aaactacgaa gagtttgtac aaatgatgac
     9541 agcgaagtga agctcgaatt aattcatcga tgatgatcca gacatgataa gatacattga
     9601 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     9661 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     9721 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     9781 aaacctctac aaatgtggta tggctgatta tgatcctcta gatcagatcc gaagatacgg
     9841 ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga
     9901 atcacatact tcctccattt tcttccactg attgactgtt ataatttccc taatttccag
     9961 gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat
    10021 gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac
    10081 atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata
    10141 gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta
    10201 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa
    10261 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa
    10321 attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga
    10381 tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
    10441 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
    10501 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
    10561 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10621 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10681 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10741 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat c
//
