LOCUS       sequence-414427-        9652 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the R2Bm ORF in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9652)
  AUTHORS   Wilkinson ME, Frangieh CJ, Macrae RK, Zhang F
  TITLE     Structure of the R2 non-LTR retrotransposon initiating target-primed
            reverse transcription.
  JOURNAL   Science. 2023 Apr 21;380(6642):301-308. doi: 
            10.1126/science.adg7883. Epub 2023 Apr 6.
  PUBMED    37023171
REFERENCE   2  (bases 1 to 9652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.adg7883"; journalName: "Science"; date: 
            "2023-04-21- 21"; volume: "380"; issue: "6642"; pages: "301-308"
COMMENT     Sequence Label: pMW41: pET-His-MBP-SUMO-R2Bm-SII
FEATURES             Location/Qualifiers
     source          1..9652
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(582..1373,1374..1442))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1205..1224
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1443..1535)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1616..2204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2105..2124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2358..2375
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2390..2529
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2455..2474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2615..2637
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2631..2822)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3394..4476)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4438..4457
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4477..4554)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4760..4779
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4867..4886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4867..4885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4886..4910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4925..4947
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5032..6129
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="KTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEE
                     KFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLI
                     AYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAA
                     DGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGET
                     AMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLE
                     NYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAFW
                     YAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      5986..5995
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     6103..6126
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             join(9457..9480,9481..9516,9517..9540)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     primer_bind     complement(9566..9584)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9580..9627
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttctg gcggcacgat
      481 ggcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      541 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      601 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
      661 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
      721 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
      781 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
      841 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
      901 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
      961 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1021 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1081 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1141 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1201 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1261 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1321 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1381 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1441 atactcttcc tttttcaatc atgattgaag catttatcag ggttattgtc tcatgagcgg
     1501 atacatattt gaatgtattt agaaaaataa acaaataggt catgaccaaa atcccttaac
     1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1741 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgtgtt tcgccacctc tgacttgagc
     2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2401 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2461 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2521 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2581 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2641 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2701 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2761 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2821 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2881 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2941 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     3001 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     3061 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3121 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3181 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3241 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc tagtcatgcc ccgcgcccac
     3301 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     3361 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     3421 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3481 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     3541 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     3601 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     3661 tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     3721 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     3781 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     3841 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     3901 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     3961 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     4021 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     4081 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     4141 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     4201 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     4261 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     4321 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     4381 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     4441 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     4501 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     4561 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     4621 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     4681 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     4741 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     4801 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg
     4861 cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa
     4921 taattttgtt taactttaag aaggagatat accatgagca agcatcacca tcattcaggc
     4981 catcaccata ccggacacca ccatcattca ggcagtcatc accataccgg gaaaactgaa
     5041 gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc
     5101 ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg
     5161 gaagagaaat tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca
     5221 cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa
     5281 gcgttccagg acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg
     5341 attgcttacc cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg
     5401 aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt
     5461 aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct
     5521 gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg
     5581 gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac
     5641 atgaatgcag acactgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg
     5701 atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt
     5761 gtaacggtac tgccgacctt caagggtcaa ccatccaaac cgttcgttgg cgtgctgagc
     5821 gcaggtatta acgccgccag tccgaacaaa gagctggcaa aagagttcct cgaaaactat
     5881 ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg tgccgtagcg
     5941 ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac catggaaaac
     6001 gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg gtatgccgtg
     6061 cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc cctgaaagac
     6121 gcgcagacta attcgagctc agctgcgggt ggcgaagaag ataagaaacc ggcaggtggc
     6181 gaaggtggcg gtgcccatat caacctgaaa gtgaaaggtc aagacggcaa cgaagtcttt
     6241 ttccgcatca aacgttctac ccagctgaaa aagctgatga acgcatactg tgaccgtcag
     6301 tctgtagaca tgaccgcaat tgctttcctg tttgatggtc gtcgcctgcg tgcggaacag
     6361 accccggatg aactggagat ggaagatggc gacgaaatcg acgcaatgct tcatcagact
     6421 ggtggctcaa gtggatccgg ttggacctgc cagttctgcg aacgaacctt ttcgaccaac
     6481 aggggtttgg gtgtccacaa gcgtagagcc caccctgttg agaccaatac ggatgccgct
     6541 ccgatgatgg tgaagcggcg gtggcatggc gaggaaatcg acctcctcgc tcgcaccgag
     6601 gccaggttgc tcgctgagcg gggtcagtgc tcgggtggag acctctttgg cgcgcttcca
     6661 gggtttggaa gaactctgga agcgattaag ggacaacggc ggagggagcc ttatcgggca
     6721 ttggtgcaag cgcaccttgc ccgatttggt tcccagccgg gtccctcgtc gggggggtgc
     6781 tcggccgagc ctgacttccg gcgggcttct ggagctgagg aagcgggcga ggaacgatgc
     6841 gccgaagacg ccgctgccta tgatccatcc gcagtcggtc agatgtcgcc cgatgccgct
     6901 cgggttctct ccgaactcct tgagggtgcg gggagaagac gagcgtgcag ggctatgaga
     6961 cccaagactg cagggcggcg aaacgatttg cacgatgatc ggacagctag tgcccacaaa
     7021 accagtagac aaaagcgcag ggcagagtac gcgcgtgtgc aggaactgta caagaagtgt
     7081 cgcagcagag cagcagctga ggtgatcgat ggcgcgtgtg ggggtgtcgg acactcgctc
     7141 gaggagatgg agacctattg gcgacctatc ctcgagagag tgtccgatgc acctgggcct
     7201 acaccggaag ctcttcacgc cctagggcgt gcggagtggc acgggggcaa tcgcgactac
     7261 acccagctgt ggaagccgat ctcggtggaa gagatcaagg cctcccgctt tgactggcga
     7321 acttcgccgg gcccggacgg tatacgttcg ggtcagtggc gtgcggttcc tgtgcacttg
     7381 aaggcggaaa tgttcaatgc atggatggca cgaggcgaaa tacccgaaat tctacggcag
     7441 tgccgaaccg tctttgtacc taaggtggag agaccaggtg gaccggggga atatcgaccg
     7501 atctcgatcg cgtcgattcc cctgagacac tttcactcca tcttggcccg gaggctgttg
     7561 gcttgctgcc cccctgatgc acgacagcgc ggatttatct gcgccgacgg tacgctggag
     7621 aattccgcag tactggacgc ggtgcttggg gatagcagga agaagctgcg ggaatgtcac
     7681 gtggcggtgc tagacttcgc caaggcattt gacacagtgt ctcacgaggc acttgtcgaa
     7741 ttgctgaggt tgaggggcat gcccgaacag ttctgcggct acattgctca cctatacgat
     7801 acggcgtcca ccaccttagc cgtgaacaat gaaatgagca gccctgtaaa agtgggacga
     7861 ggggttcgtc aaggggaccc tctgtcgccg atactcttca acgtggtgat ggacctcatc
     7921 ctggcttccc tgccggagag ggtcgggtat aggttggaga tggaactcgt gtccgctctg
     7981 gcctatgctg acgacctagt cctgcttgcg gggtcgaagg tagggatgca ggagtccatc
     8041 tctgctgtgg actgtgtcgg taggcagatg ggcctacgcc tgaattgcag gaaaagcgcg
     8101 gttctgtcta tgataccgga tggccaccgc aagaagcatc actacctgac tgagcgaacc
     8161 ttcaatattg gaggtaagcc gctcaggcag gtgagttgtg ttgagcggtg gcgatatctt
     8221 ggtgtcgatt ttgaggcctc tggatgcgtg acattagagc atagtatcag tagtgctctg
     8281 aataacatct caagggcacc tctcaaaccc caacagaggt tggagatttt gagagctcat
     8341 ctgattccga gattccagca cggttttgtg cttggaaaca tctcggatga ccgattgaga
     8401 atgctcgatg tccaaatccg gaaagcagtc ggacagtggc taaggctacc ggcggatgtg
     8461 cccaaggcat attatcacgc cgcagttcag gacggcggct tagcgatccc atcggtgcga
     8521 gcgaccatcc cggacctcat tgtgaggcgt ttcggggggc tcgactcgtc accatggtca
     8581 gtggcaagag ccgccgccaa atctgataag attcgtaaga aactgcggtg ggcctggaaa
     8641 cagctccgca ggttcagccg tgttgactcc acaacgcaac gaccatctgt gcgcttgttt
     8701 tggcgagaac atctgcatgc atctgttgat ggacgcgaac ttcgcgaatc cacacgcacc
     8761 ccgacatcca caaagtggat tagggagcga tgcgcgcaga taaccggacg ggacttcgtg
     8821 cagttcgtgc acactcatat caacgccctc ccatcccgca ttcgcggatc gagagggcgt
     8881 agaggtgggg gtgagtcttc gttgacctgc cgtgctggtt gcaaggttag ggagacgacg
     8941 gctcacatcc tacaacagtg tcacagaaca cacggcggcc ggattctacg acacaacaag
     9001 attgtatctt tcgtggcgaa agccatggaa gagaacaagt ggacggttga gctggagccg
     9061 aggctacgaa catcggttgg tctccgtaag ccggatatta tcgcctccag ggatggtgtc
     9121 ggagtgatcg tggacgtgca ggtggtctcg ggccagcgat cgcttgacga gctccaccgt
     9181 gagaaacgta ataaatacgg gaatcacggg gagctggttg agttggtcgc aggtagacta
     9241 ggacttccga aagctgagtg cgtgcgagcc acttcgtgca cgatatcttg gaggggagta
     9301 tggagcctga cttcttataa ggagttaagg tccataatcg ggcttcggga accgacacta
     9361 caaatcgttc cgatactggc gttgagaggt tcacacatga actggaccag gttcaatcag
     9421 atgacgtccg tcatgggggg cggcgttggt gctagctgga gccatccgca gtttgaaaaa
     9481 ggtggtggta gcggtggtgg ttcaggtggt agtgcatggt cacaccctca gtttgagaaa
     9541 aattaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     9601 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
//
