LOCUS       sequence-414429-       10186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing QuasAr6a_Citrine with an alternate codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Tian H, Davis HC, Wong-Campos JD, Park P, Fan LZ, Gmeiner B, Begum 
            S, Werley CA, Borja GB, Upadhyay H, Shah H, Jacques J, Qi Y, Parot 
            V, Deisseroth K, Cohen AE
  TITLE     Video-based pooled screening yields improved far-red genetically 
            encoded voltage indicators.
  JOURNAL   Nat Methods. 2023 Jul;20(7):1082-1094. doi: 
            10.1038/s41592-022-01743-5. Epub 2023 Jan 9.
  PUBMED    36624211
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-022-01743-5"; journalName: "Nat Methods"; date: 
            "2023-07"; volume: "20"; issue: "7"; pages: "1082-1094"
COMMENT     Sequence Label: HT121_Fsyn_QuasAr6a(Q3_codon)_citrine
FEATURES             Location/Qualifiers
     source          1..10186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2632..3079
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             join(3924..3926,3927..4637)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3966..3987)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4227..4246)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4574..4595
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4878..5466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4931..4951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5349..5360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5469..5485)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5470..5486)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5991..6171
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6197..6214)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6203..6427
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6473..6901
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6560..6579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6770..6791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6910..6930)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6915..7244
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7095..7230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7157..7176
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7292..7339
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7358..7732
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7862..7983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7899..7918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7953..7972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8032..8048)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8032..8048)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8045..8067)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8056..8072
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8080..8086,8087..8104,8105..8110))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8125..8146
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8263..8280)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8434..9022)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8514..8533)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9193..9984,9985..10053))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9816..9835
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10054..10158)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agtgtctaga ctgcagaggg ccctgcgtat gagtgcaagt
     2641 gggttttagg accaggatga ggcggggtgg gggtgcctac ctgacgaccg accccgaccc
     2701 actggacaag cacccaaccc ccattcccca aattgcgcat cccctatcag agagggggag
     2761 gggaaacagg atgcggcgag gcgcgtgcgc actgccagct tcagcaccgc ggacagtgcc
     2821 ttcgcccccg cctggcggcg cgcgccaccg ccgcctcagc actgaaggcg cgctgacgtc
     2881 actcgccggt cccccgcaaa ctccccttcc cggccacctt ggtcgcgtcc gcgccgccgc
     2941 cggcccagcc ggaccgcacc acgcgaggcg cgagataggg gggcacgggc gcgaccatct
     3001 gcgctgcggc gccggcgact cagcgctgcc tcagtctgcg gtgggcagcg gaggagtcgt
     3061 gtcgtgcctg agagcgcagt cgagaaggat ccaccatggt aagtatcgct ctgcaggctg
     3121 gttacgacct actgggtgac ggcagacctg aaagcctgtg gctgggcatc ggcactctgc
     3181 tgatgctgat tggaaccttc tactttctgg tccgcgccgg cggagagacc gataaggatg
     3241 cccgggaata ttacgctgtg actatcctgg tgtccggaat cgcatccgcc gcatatctgt
     3301 ctatgttctt tggtatcggg cttactgagg tgcccgtcgg gggcgaaatc ttgaacatct
     3361 attatgccag gtacgcccag tggctgctga ccaccccact tctgctgctg cacctggccc
     3421 ttctcgctaa ggtggatcgg gtgaccatcg gcaccctggt gggtggcgac gccctgatga
     3481 tcgtcactgg cctcatcgga accttgagcc acacggccat agccagatac agttggtgct
     3541 tgttctctac aatttgcatg atagtggtgc tctatgttct ggctacatcc ctgcgatctg
     3601 ctgcaaggga gcggggcccc gaggtggcat ctacctttaa catcctgaca gctctggtct
     3661 tggtgctgtg gaccgcttac cctatcatct ggatcatagg cactgagggc gctggcgtgg
     3721 tgggcctggg catcgaaact ctgctgttta tggtgttgga cgtgactgcc aaggtcggct
     3781 ttggctttat cctgttgaga tcccggagca ttctgcagga caccgaggca ccagaaccca
     3841 gtgccggtgc cgatacgttg atgaagagta gaatcacaag cgaaggcgag tacatccccc
     3901 tggatcaaat agacataaat gtagtgagca agggcgagga gctgttcacc ggggtggtgc
     3961 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4021 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4081 tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc
     4141 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4201 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4261 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4321 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4381 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     4441 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4501 tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg
     4561 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4621 tggacgagct gtacaaatca aggatcacct ctgagggcga gtatatccct ctggatcaga
     4681 tcgacattaa cgtcggtgga aaatcccgca taacctctga gggcgagtac atccctctgg
     4741 atcaaatcga tataaacgtg gggggaaaaa gtcgaatcac ttctgagggc gagtatatac
     4801 ccctcgatca gattgacatc aatgtggggg gcttttgtta tgagaatgaa taagaattcg
     4861 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     5281 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5461 ccccgcatcg ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca
     5521 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     5581 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5641 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5701 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     5761 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     5821 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     5881 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     5941 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     6001 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     6061 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     6121 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     6181 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     6241 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     6301 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     6361 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     6421 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     6481 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6541 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6601 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6661 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6721 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6781 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6841 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6901 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     6961 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     7021 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     7081 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7141 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     7201 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     7261 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
     7321 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
     7381 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
     7441 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
     7501 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
     7561 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
     7621 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
     7681 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
     7741 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     7801 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     7861 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7981 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
     8041 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     8101 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     8161 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     8221 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     8281 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8341 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8401 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8461 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8521 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8581 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8641 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8701 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8761 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     8821 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     8881 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     8941 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9001 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9061 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9121 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9181 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9241 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9301 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9361 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9421 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9481 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9541 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9601 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9661 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9721 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9781 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     9841 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     9901 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     9961 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10021 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10081 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10141 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
