LOCUS       sequence-414430-        8957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for RC-I-1-H11 (de novo virus-like particle 
            designed by David Baker lab) with N-terminal HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8957)
  TITLE     Expression Plasmids for Production of Particles Decorated with 
            HaloTag
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EP002: pLenti CMV Blast::HaloTag-RC-I-1-H11
FEATURES             Location/Qualifiers
     source          1..8957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(92..139)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(187..516)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      201..336
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     501..521
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     misc_feature    572..1160
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             678..689
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1087..1107
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1191..1215
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2394..2418
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2487..2511)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2595..2619)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(2616..2819)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2645..2665)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2820..3123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    3146..3262
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(3529..3573)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3758..3991
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4484..4609
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4656..4836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4837..5063)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5091..5111)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5091..5109)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5130..5146)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5130..5146)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5143..5165)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5154..5170
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5178..5184,5185..5202,5203..5208))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5223..5244
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5361..5378)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5532..6120)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5612..5631)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6291..7082,7083..7151))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6914..6933
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7152..7256)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7282..7737)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7419..7440)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7631..7650
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7864..7886
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7878..7895
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7879..7895
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7905..7924
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7905..7923
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7944..8079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8119..8240
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8130..8149)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8184..8203
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             8312..8545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(join(8632..8957,1..73))
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 tggggatgct gttgattgta gccgttgctc tttcaatgag ggtggattct tcttgagaca
       61 aaggcttggc catggtttag ttcctcacct tgtcgtatta tactatgccg atatactatg
      121 ccgatgatta attgtcaaca cgtgctgatc agatccgaaa atggatatac aagctcccgg
      181 gagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
      241 agaggcagag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
      301 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
      361 ggttgctgac taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga
      421 ctttccacac ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      481 ggagcctggg gactttccac accctaactg acacacattc cacagaatta attccaaact
      541 cattactaac cggatatctg cagaattcca ggcggggagg cggcccaaag ggagatccga
      601 ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag caggccgcgg
      661 gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt cccgcgcaga
      721 atccaggtgg caacacaggc gagcagccat ggaaaggacg tcagcttccc cgacaacacc
      781 acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag gcaggcggcg
      841 atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag gagctgacag
      901 gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca caccacgcca
      961 cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga tttatacaag
     1021 gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat taaagcagcg
     1081 tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct ttcacaaatt
     1141 ttgtaatcca gaggttgatt gtcgagcggc cgccactgtg ctggatatca accactttgt
     1201 acaagaaagc tgggtctagt cacagcctcc gaaaaacggt ctcagcaatc aatctagcca
     1261 gtgggtcccc ggattccagc agttcgcgaa gtgcggcaaa gagggcaggg tcaccttcct
     1321 ccaggagtcg ctccaagagt ctcagcactt cgtcgggcct acccagctcc agcaggaatc
     1381 ttgcctcggc tgcagccaaa tgtctccttc tctcttcttc catggaaccg ccagagccac
     1441 cagaaccacc ggaacctcca gaaccaccac ttcccccaga tccgccagtt gtaggttcgc
     1501 cagaaatctc cagtgtactc agccacctag ctatttctga accaatgagg tccgggttat
     1561 cctcttgcag gaggttcagg ccgggcccaa tatcgacggc cttgcagttg gggagggact
     1621 ttgcaagccg tgcagcttca gctggaggaa tcagcactcc gggtgttccc caaaacagca
     1681 gtttggggac gggagattga tggagccagt ccatatactc ttccaccaag gcgacgatgt
     1741 tagcgggttc gccggcgatg ggcaattcgt ttggaaacct ccacagaggc tcgcggtcca
     1801 ctgggttcag gaaaggctcg cggtaatggt ccatctccac ttcggtcaag ggtcgcacga
     1861 cacccatggg caaggttccc tcgatgaaga cgttctgatc gataatcaac tttctaccca
     1921 catctgtagt ccggaaggcc tggaatgtct ccctcgcaaa ctctggccac tcatcccaag
     1981 ttggtatagg gcgaataaat tccataaaag caatcccttt taccctctct gggttgcgtt
     2041 tggcccaatg aaatccaagt gcactgcccc agtcgtgaat gaccaaaacc acctcctcca
     2101 agccaagtgc ctcaatgaag gcgtccatga acctgacgtg gtcgtcgaag aagtatccga
     2161 ggtccggttt gtcgctcttg cccataccaa tgagatcagg ggcgatacag cgatgtgtag
     2221 gagccacatg gggaatgatg tttctccata cgtaggagga agttgggttt ccgtgaagaa
     2281 acaacacagg agttccatcc ctgggtccca cgtccacgta atgcatcctc tcgccaagca
     2341 cttccacata atgggggtcg aaaggaaatc cggtcccaat catggtggag cctgcttttt
     2401 tgtacaagaa agctgggtct agatatctcg agtgcggccg cgaattcggt accggatcca
     2461 gtcgactgaa ttggttccca tggtggagcc tgcttttttg tacaaacttg ttgatatctg
     2521 cagaattcca ccacactgga ctagagtcgg tgtcttctat ggaggtcaaa acagcgtgga
     2581 tggcgtctcc aggcgatctg acggttcact aaacgagctc tgcttatata gacctcccac
     2641 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
     2701 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
     2761 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
     2821 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     2881 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     2941 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     3001 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     3061 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     3121 acgcggaact cccaagctta tcgataaaat tttgaatttt gtaatttgtt tttgtaattc
     3181 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     3241 aatcccccct tttcttttaa aagttaaccg ataccgtcga gatccgttca ctaatcgaat
     3301 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc
     3361 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     3421 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     3481 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     3541 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     3601 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     3661 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     3721 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     3781 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     3841 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     3901 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     3961 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     4021 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     4081 tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag
     4141 cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc ttgtctaaag
     4201 cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat
     4261 tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta
     4321 tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat cgttctagct
     4381 ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc
     4441 gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat
     4501 ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct
     4561 cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt
     4621 tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta
     4681 aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac acactacttg
     4741 aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag
     4801 ctcccaggct cagatctggt ctaaccagag agacccgttt atgtatcgag ctaggcactt
     4861 aaatacaata tctctgcaat gcggcaattc agtggttcgt ccaatccatg tcagacccgt
     4921 ctgttgcctt cctaataagg cacgatcgta ccaccttact tccaccaatc ggcatgcacg
     4981 gtgctttttc tctccttgta aggcatgttg ctaactcatc gttaccatgt tgcaagacta
     5041 caagagtatt gcataagact acattaagct tgcagctcca gcttttgttc cctttagtga
     5101 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5161 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5221 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5281 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5341 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5401 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5461 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5521 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5581 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5641 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5701 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5761 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5821 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5881 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5941 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6001 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6061 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6121 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6181 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6241 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6301 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6361 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6421 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6481 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6541 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6601 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6661 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6721 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6781 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6841 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6901 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6961 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7021 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7081 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7141 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7201 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7261 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7321 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7381 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7441 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7501 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7561 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     7621 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7681 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     7741 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     7801 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     7861 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     7921 agggcgaatt gggtacgtcc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     7981 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     8041 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggat
     8101 agctagagcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     8161 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     8221 taagctgcaa taaacaagtt cctctcactc tctgatattc atttctttgc aagttataaa
     8281 tactgaataa taagatgaca tgaactacta ctgctagaga ttttccacac tgactaaaag
     8341 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     8401 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     8461 caggctcaga tctggtctaa ccagagagac ccagtacaag caaaaagcag atcttgtctt
     8521 cgttgggagt gagctagccc ttccagtccc cccttttctt ttaaaaagtg gctaagatct
     8581 acagctgcct tgtaagtcat tggtcttaaa ggtaccgagc tcgaattgtg cttagccctc
     8641 ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct gtccatcact
     8701 gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc
     8761 gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag tcaggttgcc
     8821 agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc ccccagtaaa
     8881 atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg cgctggcgac
     8941 gctgtagtct tcagaga
//
