LOCUS       sequence-414434-        8676 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for RC-I-1-H11 (de novo virus-like particle 
            designed by David Baker lab) with N-terminal eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8676)
  TITLE     Expression Plasmids for Production of Particles Decorated with 
            HaloTag
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EP001: pLenti CMV Blast::eGFP-RC-I-1-H11
FEATURES             Location/Qualifiers
     source          1..8676
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     53..73
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     99..123
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    207..231
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1222..1246)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    1277..1865
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1330..1350)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1748..1759)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1916..1936)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1921..2250
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2101..2236
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        2298..2345
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             2364..2762
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             2849..3082
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3154..3275
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3191..3210)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3245..3264
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3315..3450
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3470..3489)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3471..3489)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3499..3516)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3499..3515)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3508..3530)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3657..4112
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3744..3763)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3954..3975
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4138..4242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4243..4311,4312..5103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4461..4480)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5274..5862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5763..5782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6016..6033
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6150..6171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6186..6191,6192..6209,6210..6216)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6224..6240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6229..6251
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6248..6264
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6248..6264
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6283..6303
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6285..6303
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6331..6557
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6558..6738
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6785..6910
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7403..7636
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7821..7865
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8132..8248
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        8271..8574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(8575..8676,1..102)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
ORIGIN
        1 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
       61 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag
      121 atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg actctagtcc
      181 agtgtggtgg aattctgcag atatcaacaa gtttgtacaa aaaagcaggc tccaccatgt
      241 caaaaggcga ggaactcttt acgggcgtgg tacctatcct tgtcgagctg gacggagatg
      301 tgaacggcta caaatttagt gtctctggtg agggcgaggg ggatgctacc tatgggaagc
      361 tgacccttaa atttatctgc accacaggga aattgccagt gccctggccc actctggtta
      421 caacgctcac ctatggcgtc cagtgtttca gcagataccc tgaccatatg aagcgacatg
      481 acttttttaa gagcgccatg ccagaggggt acgtccagga gcgaaccatt ttcttcaaag
      541 acgacggaaa ttacaaaaca agggcggaag tcaagttcga gggcgacacc cttgtgaatc
      601 ggattgagct gaaggggatc gatttcaaag aggatgggaa tatcctcggc cataaactgg
      661 aatataatta taactctcac aacgtgtaca tcatggcgga caagcagaag aatgggatca
      721 aagccaactt caaaactcgc cacaacattg aagatggcgg cgtgcagctg gcagatcact
      781 accagcagaa cacaccaata ggagatgacc ccgtcctgct tccagacaat cattatttgt
      841 ctacgcagtc cgcgctgtcc aaagacccta acgagaaacg ggaccacatg gtaatactcg
      901 agttcgtgac agctgctggc atcacccacg gtatggacga actgtacaag ggcggatctg
      961 ggggaagtgg tggttctgga ggttccggtg gttctggtgg ctctggcggt tccatggaag
     1021 aagagagaag gagacatttg gctgcagccg aggcaagatt cctgctggag ctgggtaggc
     1081 ccgacgaagt gctgagactc ttggagcgac tcctggagga aggtgaccct gccctctttg
     1141 ccgcacttcg cgaactgctg gaatccgggg acccactggc tagattgatt gctgagaccg
     1201 tttttcggag gctgtgacta gacccagctt tcttgtacaa agtggttgat atccagcaca
     1261 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1321 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     1381 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     1441 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     1501 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     1561 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     1621 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     1681 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     1741 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     1801 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     1861 cccgcctgga attctgcaga tatccggtta gtaatgagtt tggaattaat tctgtggaat
     1921 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     1981 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     2041 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     2101 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     2161 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     2221 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     2281 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     2341 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
     2401 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     2461 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     2521 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     2581 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     2641 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     2701 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     2761 aagcacaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     2821 agccactttt taaaagaaaa ggggggactg gaagggctag ctcactccca acgaagacaa
     2881 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2941 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3001 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3061 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     3121 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3181 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3241 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     3301 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3361 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3421 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     3481 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3541 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3601 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     3661 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3721 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3781 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3841 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     3901 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     3961 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4021 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4081 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4141 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4201 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4261 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4321 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4381 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4441 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4501 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4561 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4621 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4681 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4741 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4801 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4861 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4921 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4981 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5041 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5101 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5161 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5221 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5281 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5341 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5401 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5461 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5521 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5581 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5641 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5701 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5761 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5821 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5881 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5941 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6001 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6061 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6121 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6181 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6241 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     6301 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     6361 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     6421 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     6481 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     6541 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6601 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6661 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6721 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     6781 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     6841 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     6901 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     6961 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7021 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7081 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7141 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7201 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     7261 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     7321 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     7381 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     7441 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     7501 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     7561 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     7621 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     7681 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     7741 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     7801 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     7861 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     7921 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     7981 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     8041 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     8101 tagtgaacgg atctcgacgg tatcggttaa cttttaaaag aaaagggggg attggggggt
     8161 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     8221 aaaaacaaat tacaaaattc aaaattttat cgataagctt gggagttccg cgttacataa
     8281 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     8341 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     8401 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
     8461 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     8521 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     8581 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     8641 ctccacccca ttgacgtcaa tgggagtttg ttttgg
//
