LOCUS       sequence-414446-       11843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses presynaptic low affinity ratiometric ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: 
            plenti.CamKII.(synapto).iATPSnFR2.A95A.A119L.miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..11843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..61)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        233..612
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..812
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     767..787
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             830..1010
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1057..1182
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1675..1908
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2093..2137
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2435..2552
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2597..3885
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     regulatory      3890..3899
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     5416..5437
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5540..5561)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6530..7118
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6583..6603)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7001..7012)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7121..7137)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7122..7138)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7643..7823
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7849..7866)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7855..8079
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8125..8553
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8212..8231)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8422..8443
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8562..8582)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8567..8896
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8747..8882
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8809..8828
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8944..8991
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9010..9384
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9514..9635
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9551..9570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9605..9624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9684..9700)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9684..9700)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9697..9719)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9708..9724
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9732..9738,9739..9756,9757..9762))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9777..9798
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9915..9932)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10086..10674)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10166..10185)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10845..11636,11637..11705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11468..11487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11706..11810)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg ctctgatgcc
       61 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
      121 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      181 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      241 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      301 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      361 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      421 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      481 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      541 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      601 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      661 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      721 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      781 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      841 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      901 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      961 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     1021 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1081 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1141 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1201 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1261 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1321 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1381 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1441 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1501 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1561 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1621 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1681 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1741 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1801 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1861 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1921 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1981 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     2041 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2101 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2161 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2221 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2281 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2341 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2401 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2461 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2521 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2581 cagtttggtt aattaacatt atggccttag gtcacttcat ctccatgggg ttcttcttct
     2641 gattttctag aaaatgagat gggggtgcag agagcttcct cagtgacctg cccagggtca
     2701 catcagaaat gtcagagcta gaacttgaac tcagattact aatcttaaat tccatgcctt
     2761 gggggcatgc aagtacgata tacagaagga gtgaactcat tagggcagat gaccaatgag
     2821 tttaggaaag aagagtccag ggcagggtac atctacacca cccgcccagc cctgggtgag
     2881 tccagccacg ttcacctcat tatagttgcc tctctccagt cctaccttga cgggaagcac
     2941 aagcagaaac tgggacagga gccccaggag accaaatctt catggtccct ctgggaggat
     3001 gggtggggag agctgtggca gaggcctcag gaggggccct gctgctcagt ggtgacagat
     3061 aggggtgaga aagcagacag agtcattccg tcagcattct gggtctgttt ggtacttctt
     3121 ctcacgctaa ggtggcggtg tgatatgcac aatggctaaa aagcagggag agctggaaag
     3181 aaacaaggac agagacagag gccaagtcaa ccagaccaat tcccagagga agcaaagaaa
     3241 ccattacaga gactacaagg gggaagggaa ggagagatga attagcttcc cctgtaaacc
     3301 ttagaaccca gctgttgcca gggcaacggg gcaatacctg tctcttcaga ggagatgaag
     3361 ttgccagggt aactacatcc tgtctttctc aaggaccatc ccagaatgtg gcacccacta
     3421 gccgttacca tagcaactgc ctctttgccc cacttaatcc catcccgtct gttaaaaggg
     3481 ccctatagtt ggaggtgggg gaggtaggaa gagcgatgat cacttgtgga ctaagtttgt
     3541 tcgcatcccc ttctccaacc ccctcagtac atcaccctgg gggaacaggg tccacttgct
     3601 cctgggccca cacagtcctg cagtattgtg tatataaggc cagggcaaag aggagcaggt
     3661 tttaaagtga aaggcaggca ggtgttgggg aggcagttac cggggcaacg ggaacagggc
     3721 gtttcggagg tggttgccat ggggacctgg atgctgacga aggctcgcga ggctgtgagc
     3781 agccacagtg ccctgctcag aagccccaag ctcgtcagtc aagccggttc tccgtttgca
     3841 ctcaggagca cgggcaggcg agtggcccct agttctgggg gcagcggggg ccaccatgga
     3901 cgtggtgaat cagctggtgg ctgggggtca gttccgggtg gtcaaggagc cccttggctt
     3961 cgtgaaggtg ctgcagtggg tctttgccat cttcgccttt gctacgtgtg gcagctacac
     4021 cggggagctt cggctgagcg tggagtgtgc caacaagacg gagagtgccc tcaacatcga
     4081 agttgaattc gagtacccct tcaggctgca ccaagtgtac tttgatgcac cctcctgcgt
     4141 caaagggggc actaccaaga tcttcctggt tggggactac tcctcgtcgg ctgaattctt
     4201 tgtcaccgtg gctgtgtttg ccttcctcta ctccatgggg gccctggcca cctacatctt
     4261 cctgcagaac aagtaccgag agaacaacaa agggcctatg atggactttc tggctacagc
     4321 cgtgttcgct ttcatgtggc tagttagttc atcagcctgg gccaaaggcc tgtccgatgt
     4381 gaagatggcc acggacccag agaacattat caaggagatg cccatgtgcc gccagacagg
     4441 gaacacatgc aaggaactga gggaccctgt gacttcagga ctcaacacct cagtggtgtt
     4501 tggcttcctg aacctggtgc tctgggttgg caacttatgg ttcgtgttca aggagacagg
     4561 ctgggcagcc ccattcatgc gcgcacctcc aggcgccccg gaaaagcaac cagcacctgg
     4621 cgatgcctac ggcgatgcgg gctacgggca gggccccgga ggctatgggc cccaggactc
     4681 ctacgggcct cagggtggtt atcaacccga ttacgggcag ccagccagcg gtggcggtgg
     4741 ctacgggcct cagggcgact atgggcagca aggctatggc caacagggtg cgcccacctc
     4801 cttctccaat cagatgggat ccactagtaa cggccgccag tgtgctggaa ttctgcagat
     4861 atcgatgggc agatctatga agactattca cgtgagtgtc gtaactcccg acgggcctgt
     4921 atatgaggat gacgttgaaa tggtgagcgt caaagcaaaa agtggcgagc tcggtattct
     4981 cccaggccac attcccttga aagctcccct agagatcagt gccgcacgcc tgaagaaagg
     5041 gggcaaaaca cagtatatcg ctgtgtcagg cggcaacctc gaagtgcggc ctgacaaggt
     5101 gaccatctat gcccaggcag ccgagagggc tgaggatatc gatgtcctgc gcgccaaggc
     5161 cgccaaggag agagccgagc gccgactcca atcacaggtc ctggtcggga acgtctatat
     5221 caccgccgac aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacgtgga
     5281 ggacggcagc atgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     5341 cgtgctgctg cccgacaacc actacctgag cacccagtcc gtgctgagca aagaccctaa
     5401 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5461 catggacgag ctgtacaagg gcggtaccgg agggagcatg agcaagggcg aggagctgtt
     5521 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     5581 cgtgcgcggc gagggcgagg gcgatgccac caacggcaag ctgaccctga agttcatctg
     5641 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     5701 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     5761 gcccgaaggc tacgtccagg agcgcaccat cagcttcaag gacgacggca cctacaagac
     5821 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5881 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact ttatttgtgt
     5941 ggacatcgat tttaagcgcg ccgagctctt gcttaagcgc gcaatgaata ggctctcagt
     6001 tgccgaaatg aagctgcagg gcggaggctc gggcggaggt gggtcgggtg gcggcggatc
     6061 aatggcaaac ctggacaaga tgctgaacac caccgtgacc gaggtgcgca agttcctgca
     6121 agcggacaga gtgtgcgtgt tcaagttcga ggaagattac tccggcaccg tctcgcacga
     6181 agccgtggac gacagatgga ttagcatcct gaagacccag gtgcaggaca gatacttcat
     6241 ggaaaccaga ggcgaggaat acgtccacgg cagataccag gccatcgccg acatctacac
     6301 agccaatctg gtcgagtgct acagagacct gctgatcgag tttcaggtgc gggccattct
     6361 ggctgtcccc atcctgcaag gcaagaagct gtggggcctg ctggtggccc accaactggc
     6421 cggccctcgg gagtggcaga cctgggaaat cgacttcctg aaacagcaag ccgtggtgat
     6481 gggcatcgcc atccagcaga gctaggaatt cgatatcaag cttatcgata atcaacctct
     6541 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     6601 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     6661 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     6721 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     6781 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     6841 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     6901 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     6961 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     7021 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     7081 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
     7141 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     7201 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     7261 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7321 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     7381 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     7441 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     7501 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     7561 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     7621 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7681 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7741 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7801 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     7861 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     7921 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7981 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     8041 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     8101 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8161 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8221 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8281 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8341 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8401 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8461 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8521 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     8581 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     8641 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8701 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8761 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8821 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8881 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     8941 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     9001 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     9061 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     9121 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     9181 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     9241 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     9301 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     9361 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     9421 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     9481 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     9541 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     9601 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     9661 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9721 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9781 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9841 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9901 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9961 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    10021 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10081 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10141 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10201 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10261 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10321 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10381 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10441 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10501 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10561 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10621 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10681 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10741 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10801 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10861 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10921 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10981 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11041 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11101 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11161 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11221 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11281 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11341 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11401 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11461 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11521 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11581 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11641 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11701 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11761 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11821 cgaaaagtgc cacctgacgt cga
//
