LOCUS       sequence-414451-       10867 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cytosolic medium affinity ratiometric ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: plenti.CamKII.(cyto).iATPSnFR2.A95K.miRFP670nano3
FEATURES             Location/Qualifiers
     source          1..10867
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..72)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        244..623
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        625..823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     778..798
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             841..1021
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1068..1193
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2104..2148
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2446..2563
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2608..3896
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     4451..4472
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4575..4596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    5565..6153
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5618..5638)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6036..6047)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6156..6172)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6157..6173)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6678..6858
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6884..6901)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6890..7114
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7160..7588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7247..7266)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7457..7478
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7597..7617)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7602..7931
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7782..7917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7844..7863
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7979..8026
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8045..8419
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8549..8670
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8586..8605)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8640..8659
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8719..8735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8719..8735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8732..8754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8743..8759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8767..8773,8774..8791,8792..8797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8812..8833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8950..8967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9121..9709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9201..9220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9880..10671,10672..10740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10503..10522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10741..10845)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
       61 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
      121 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
      181 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
      241 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
      301 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      361 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      421 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      481 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      541 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      601 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
      661 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      721 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      781 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
      841 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
      901 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      961 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     1021 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     1081 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     1141 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     1201 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     1261 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     1321 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     1381 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     1441 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     1501 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt taattaacat tatggcctta ggtcacttca tctccatggg
     2641 gttcttcttc tgattttcta gaaaatgaga tgggggtgca gagagcttcc tcagtgacct
     2701 gcccagggtc acatcagaaa tgtcagagct agaacttgaa ctcagattac taatcttaaa
     2761 ttccatgcct tgggggcatg caagtacgat atacagaagg agtgaactca ttagggcaga
     2821 tgaccaatga gtttaggaaa gaagagtcca gggcagggta catctacacc acccgcccag
     2881 ccctgggtga gtccagccac gttcacctca ttatagttgc ctctctccag tcctaccttg
     2941 acgggaagca caagcagaaa ctgggacagg agccccagga gaccaaatct tcatggtccc
     3001 tctgggagga tgggtgggga gagctgtggc agaggcctca ggaggggccc tgctgctcag
     3061 tggtgacaga taggggtgag aaagcagaca gagtcattcc gtcagcattc tgggtctgtt
     3121 tggtacttct tctcacgcta aggtggcggt gtgatatgca caatggctaa aaagcaggga
     3181 gagctggaaa gaaacaagga cagagacaga ggccaagtca accagaccaa ttcccagagg
     3241 aagcaaagaa accattacag agactacaag ggggaaggga aggagagatg aattagcttc
     3301 ccctgtaaac cttagaaccc agctgttgcc agggcaacgg ggcaatacct gtctcttcag
     3361 aggagatgaa gttgccaggg taactacatc ctgtctttct caaggaccat cccagaatgt
     3421 ggcacccact agccgttacc atagcaactg cctctttgcc ccacttaatc ccatcccgtc
     3481 tgttaaaagg gccctatagt tggaggtggg ggaggtagga agagcgatga tcacttgtgg
     3541 actaagtttg ttcgcatccc cttctccaac cccctcagta catcaccctg ggggaacagg
     3601 gtccacttgc tcctgggccc acacagtcct gcagtattgt gtatataagg ccagggcaaa
     3661 gaggagcagg ttttaaagtg aaaggcaggc aggtgttggg gaggcagtta ccggggcaac
     3721 gggaacaggg cgtttcggag gtggttgcca tggggacctg gatgctgacg aaggctcgcg
     3781 aggctgtgag cagccacagt gccctgctca gaagccccaa gctcgtcagt caagccggtt
     3841 ctccgtttgc actcaggagc acgggcaggc gagtggcccc tagttctggg ggcagcgggg
     3901 gatccgccac catgaagact attcacgtga gtgtcgtaac tcccgacggg cctgtatatg
     3961 aggatgacgt tgaaatggtg agcgtcaaag caaaaagtgg cgagctcggt attctcccag
     4021 gccacattcc cttgaaagct cccctagaga tcagtgccgc acgcctgaag aaagggggca
     4081 aaacacagta tatcgctgtg tcaggcggca acctcgaagt gcggcctgac aaggtgacca
     4141 tctatgccca ggcagccgag agggctgagg atatcgatgt cctgcgcgcc aagaaagcca
     4201 aggagagagc cgagcgccga ctccaatcac aggtcctggt cgggaacgtc tatatcaccg
     4261 ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac gtggaggacg
     4321 gcagcatgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     4381 tgctgcccga caaccactac ctgagcaccc agtccgtgct gagcaaagac cctaacgaga
     4441 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     4501 acgagctgta caagggcggt accggaggga gcatgagcaa gggcgaggag ctgttcaccg
     4561 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc
     4621 gcggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca
     4681 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     4741 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     4801 aaggctacgt ccaggagcgc accatcagct tcaaggacga cggcacctac aagacccgcg
     4861 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     4921 tcaaggagga cggcaacatc ctggggcaca agctggagta caactttatt tgtgtggaca
     4981 tcgattttaa gcgcgccgag ctcgccctta agcgcgcaat gaataggctc tcagttgccg
     5041 aaatgaagct gcagggcgga ggctcgggcg gaggtgggtc gggtggcggc ggatcaatgg
     5101 caaacctgga caagatgctg aacaccaccg tgaccgaggt gcgcaagttc ctgcaagcgg
     5161 acagagtgtg cgtgttcaag ttcgaggaag attactccgg caccgtctcg cacgaagccg
     5221 tggacgacag atggattagc atcctgaaga cccaggtgca ggacagatac ttcatggaaa
     5281 ccagaggcga ggaatacgtc cacggcagat accaggccat cgccgacatc tacacagcca
     5341 atctggtcga gtgctacaga gacctgctga tcgagtttca ggtgcgggcc attctggctg
     5401 tccccatcct gcaaggcaag aagctgtggg gcctgctggt ggcccaccaa ctggccggcc
     5461 ctcgggagtg gcagacctgg gaaatcgact tcctgaaaca gcaagccgtg gtgatgggca
     5521 tcgccatcca gcagagctag gaattcgata tcaagcttat cgataatcaa cctctggatt
     5581 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5641 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5701 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5761 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5821 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5881 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5941 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     6001 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     6061 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     6121 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag
     6181 aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc
     6241 tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac
     6301 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     6361 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     6421 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     6481 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     6541 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     6601 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     6661 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6841 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     6901 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     6961 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     7021 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     7081 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     7141 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     7201 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     7261 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     7321 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     7381 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     7441 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     7501 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     7561 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     7621 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7681 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7741 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     7801 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     7861 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     7921 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
     7981 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
     8041 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
     8101 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
     8161 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
     8221 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
     8281 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
     8341 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
     8401 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
     8461 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     8521 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     8581 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     8641 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     8701 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8761 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8821 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8881 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8941 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9001 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9061 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9121 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9181 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9241 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9301 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9361 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9421 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9481 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9541 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9601 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9661 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9721 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9781 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9841 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9901 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9961 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10021 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10081 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10141 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10201 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10261 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10321 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10381 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10441 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10501 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10561 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10621 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10681 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10741 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10801 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10861 agtgcca
//
