LOCUS       sequence-414454-       11317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cytosolic low affinity ratiometric ATP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Marvin JS, Kokotos AC, Kumar M, Pulido C, Tkachuk AN, Yao JS, Brown 
            TA, Ryan TA
  TITLE     iATPSnFR2: A high-dynamic-range fluorescent sensor for monitoring 
            intracellular ATP.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 May 21;121(21):e2314604121. doi: 
            10.1073/pnas.2314604121. Epub 2024 May 15.
  PUBMED    38748581
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-05-21"; volume: "121(21):e2314604121. 
            doi"; pages: " 10.1073/pnas.2314604121. Epub 2024 May 15"
COMMENT     Sequence Label: plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag
FEATURES             Location/Qualifiers
     source          1..11317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     770..790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             833..1013
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1060..1185
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2096..2140
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2438..2555
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2600..3888
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     primer_bind     4443..4464
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4567..4588)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6007..6595
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6060..6080)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6478..6489)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6598..6614)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6599..6615)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7120..7300
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7326..7343)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7332..7556
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7602..8030
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7689..7708)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7899..7920
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8039..8059)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8044..8373
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8224..8359
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8286..8305
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8421..8468
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8487..8861
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8991..9112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9028..9047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9082..9101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9161..9177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9161..9177)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9174..9196)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9185..9201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9209..9215,9216..9233,9234..9239))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9254..9275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9392..9409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9563..10151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9643..9662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10322..11113,11114..11182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10945..10964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11183..11287)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct
      841 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      901 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      961 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     1021 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     1081 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     1141 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     1201 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     1261 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     1321 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     1381 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     1441 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     1501 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     2401 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     2461 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2521 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     2581 atccagtttg gttaattaac attatggcct taggtcactt catctccatg gggttcttct
     2641 tctgattttc tagaaaatga gatgggggtg cagagagctt cctcagtgac ctgcccaggg
     2701 tcacatcaga aatgtcagag ctagaacttg aactcagatt actaatctta aattccatgc
     2761 cttgggggca tgcaagtacg atatacagaa ggagtgaact cattagggca gatgaccaat
     2821 gagtttagga aagaagagtc cagggcaggg tacatctaca ccacccgccc agccctgggt
     2881 gagtccagcc acgttcacct cattatagtt gcctctctcc agtcctacct tgacgggaag
     2941 cacaagcaga aactgggaca ggagccccag gagaccaaat cttcatggtc cctctgggag
     3001 gatgggtggg gagagctgtg gcagaggcct caggaggggc cctgctgctc agtggtgaca
     3061 gataggggtg agaaagcaga cagagtcatt ccgtcagcat tctgggtctg tttggtactt
     3121 cttctcacgc taaggtggcg gtgtgatatg cacaatggct aaaaagcagg gagagctgga
     3181 aagaaacaag gacagagaca gaggccaagt caaccagacc aattcccaga ggaagcaaag
     3241 aaaccattac agagactaca agggggaagg gaaggagaga tgaattagct tcccctgtaa
     3301 accttagaac ccagctgttg ccagggcaac ggggcaatac ctgtctcttc agaggagatg
     3361 aagttgccag ggtaactaca tcctgtcttt ctcaaggacc atcccagaat gtggcaccca
     3421 ctagccgtta ccatagcaac tgcctctttg ccccacttaa tcccatcccg tctgttaaaa
     3481 gggccctata gttggaggtg ggggaggtag gaagagcgat gatcacttgt ggactaagtt
     3541 tgttcgcatc cccttctcca accccctcag tacatcaccc tgggggaaca gggtccactt
     3601 gctcctgggc ccacacagtc ctgcagtatt gtgtatataa ggccagggca aagaggagca
     3661 ggttttaaag tgaaaggcag gcaggtgttg gggaggcagt taccggggca acgggaacag
     3721 ggcgtttcgg aggtggttgc catggggacc tggatgctga cgaaggctcg cgaggctgtg
     3781 agcagccaca gtgccctgct cagaagcccc aagctcgtca gtcaagccgg ttctccgttt
     3841 gcactcagga gcacgggcag gcgagtggcc cctagttctg ggggcagcgg gggatccgcc
     3901 accatgaaga ctattcacgt gagtgtcgta actcccgacg ggcctgtata tgaggatgac
     3961 gttgaaatgg tgagcgtcaa agcaaaaagt ggcgagctcg gtattctccc aggccacatt
     4021 cccttgaaag ctcccctaga gatcagtgcc gcacgcctga agaaaggggg caaaacacag
     4081 tatatcgctg tgtcaggcgg caacctcgaa gtgcggcctg acaaggtgac catctatgcc
     4141 caggcagccg agagggctga ggatatcgat gtcctgcgcg ccaaggccgc caaggagaga
     4201 gccgagcgcc gactccaatc acaggtcctg gtcgggaacg tctatatcac cgccgacaag
     4261 cagaagaacg gcatcaaggc gaacttcaag atccgccaca acgtggagga cggcagcatg
     4321 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4381 gacaaccact acctgagcac ccagtccgtg ctgagcaaag accctaacga gaagcgcgat
     4441 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4501 tacaagggcg gtaccggagg gagcatgagc aagggcgagg agctgttcac cggggtggtg
     4561 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gcgcggcgag
     4621 ggcgagggcg atgccaccaa cggcaagctg accctgaagt tcatctgcac caccggcaag
     4681 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4741 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4801 gtccaggagc gcaccatcag cttcaaggac gacggcacct acaagacccg cgccgaggtg
     4861 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4921 gacggcaaca tcctggggca caagctggag tacaacttta tttgtgtgga catcgatttt
     4981 aagcgcgccg agctcttgct taagcgcgca atgaataggc tctcagttgc cgaaatgaag
     5041 ctgcagggcg gaggctcggg cggaggtggg tcgggtggcg gcggatcaat ggccgagatc
     5101 ggcaccggct tccccttcga cccccactac gtggaggtgc tgggcgagcg catgcactac
     5161 gttgacgtgg gcccccgcga cggcaccccc gtgctgttcc tgcacggcaa ccccaccagc
     5221 agctacgtgt ggcgcaacat catcccccac gtggccccca cccaccgcac tatcgccccc
     5281 gacctgatcg gcatgggcaa gagcgacaag cccgacctgg gctacttctt cgacgaccac
     5341 gtgcgcttca tggacgcctt catcgaggcc ctgggcctgg aggaggtggt gctggtgatc
     5401 cacgactggg gcagcgccct gggcttccac tgggccaagc gcaaccccga gcgcgtgaag
     5461 ggcatcgcct tcatggagtt catccgcccc atccccacct gggacgagtg gcccgagttc
     5521 gcccgcgaga ccttccaggc cttccgcacc accgacgtgg gccgcaagct gatcatcgac
     5581 cagaacgtgt tcatcgaggg caccctgccc atgggcgtgg tgcgccccct gaccgaggtg
     5641 gagatggacc actaccgcga gcccttcctg aaccccgtgg accgcgagcc cctgtggcgc
     5701 ttccccaacg agctgcccat cgccggcgag cccgccaaca tcgtggccct ggtggaggag
     5761 tacatggact ggctgcacca gagccccgtg cccaagctgc tgttctgggg cacccccggc
     5821 gtgctgatcc cccccgccga ggccgcccgc ctggccaaga gcctgcccaa cgttaaggcc
     5881 gtggacatcg gccccggcct gaacctgctc caggaggaca accccgacct gatcggcagc
     5941 gagatcgccc gctggctgag caccctcgag atcagcggct aggaattcga tatcaagctt
     6001 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6061 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6121 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     6181 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     6241 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     6301 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     6361 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     6421 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     6481 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     6541 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     6601 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     6661 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     6721 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     6781 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     6841 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     6901 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     6961 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     7021 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     7081 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     7141 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     7201 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     7261 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     7321 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     7381 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     7441 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     7501 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     7561 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     7621 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7681 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7741 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     7801 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     7861 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     7921 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     7981 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     8041 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     8101 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     8161 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     8221 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8281 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     8341 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     8401 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     8461 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     8521 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     8581 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     8641 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     8701 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     8761 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     8821 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     8881 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     8941 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     9001 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9061 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9121 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     9181 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     9241 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     9301 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     9361 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9961 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    10021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    10081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    10261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    10381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10621 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10921 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    11041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    11101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    11161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    11221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    11281 ttccgcgcac atttccccga aaagtgccac ctgacgt
//
