LOCUS       sequence-41446-e         648 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with 
            IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..648
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(30..49)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          32..164
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(212..463)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(254..275)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     603..622
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgaca cctgtggaga gaaaggcaaa gtggatgtca
       61 gtaagaccaa taggtgccta tcagaaacgc aagagtcttc tctgtctcga caagcccagt
      121 ttctattggt ctccttaaac ctgtcttgta accttgatac ttacctgcgg cctcgacctt
      181 agctattcat cggtacctcg actctagatt acttgtacag ctcgtccatg ccgagagtga
      241 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      301 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
      361 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
      421 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
      481 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
      541 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
      601 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctgga
//
