LOCUS       sequence-414476-        9074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon 
            optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Foss DV, Muldoon JJ, Nguyen DN, Carr D, Sahu SU, Hunsinger JM, Wyman
            SK, Krishnappa N, Mendonsa R, Schanzer EV, Shy BR, Vykunta VS, 
            Allain V, Li Z, Marson A, Eyquem J, Wilson RC
  TITLE     Peptide-mediated delivery of CRISPR enzymes for the efficient 
            editing of primary human lymphocytes.
  JOURNAL   Nat Biomed Eng. 2023 May;7(5):647-660. doi: 
            10.1038/s41551-023-01032-2. Epub 2023 Apr 25.
  PUBMED    37147433
REFERENCE   2  (bases 1 to 9074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41551-023-01032-2"; journalName: "Nat Biomed Eng"; date: 
            "2023-05"; volume: "7"; issue: "5"; pages: "647-660"
COMMENT     Sequence Label: pRM053 His-TEV-Cas9 triNLS 0C
FEATURES             Location/Qualifiers
     source          1..9074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..914
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(840..859)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1000..1022
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1016..1207)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     2665..2684
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2768..2787
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2768..2786
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2875..2897
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2917..2934
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2938..2958
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             2968..2994
                     /codon_start=1
                     /product="nuclear localization signal (Makkerh et al., 
                     1996)"
                     /label=NLS
                     /translation="PAAKKKKLD"
     CDS             3007..7107
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRISYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIESFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIINLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7111..7137
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7117..7143
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             7156..7176
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7192..7239
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(7371..7389)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7385..7432
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(7798..7803,7804..7820,7821..7826))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(7853..7871)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7939..8043
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8044..8112,8113..8904)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8262..8281)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt
      841 acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact
      901 gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
      961 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1021 ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt
     1081 cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca
     1141 gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1201 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1261 aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1321 gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg
     1381 gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc
     1441 ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt
     1501 acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc
     1561 agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac
     1621 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
     1681 acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     1741 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     1801 ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc
     1861 ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac
     1921 aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag
     1981 cggtccaatg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg
     2041 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     2101 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     2161 gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     2221 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     2281 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     2341 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     2401 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     2461 catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     2521 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     2581 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     2641 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     2701 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     2761 gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctctag tgccggctcc
     2821 ggagagctct ttaattaagc ggccgccctg caggactcga gttctagaaa taattttgtt
     2881 taactttaag aaggagatat acatatgaaa tcttctcacc atcaccatca ccatgaggaa
     2941 aacctgtact tccaatcccg cgccgctccg gcagctaaga aaaagaaact ggataatgcc
     3001 accagcgaca agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc
     3061 gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac
     3121 cggcacagca tcaagaagaa cctgatcggc gccctgctgt tcgacagcgg agaaacagcc
     3181 gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc
     3241 agctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac
     3301 agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc
     3361 ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga
     3421 aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc
     3481 cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc
     3541 gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac
     3601 cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc
     3661 agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcggc
     3721 aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc
     3781 gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg
     3841 gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc
     3901 atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc
     3961 tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg
     4021 cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac
     4081 gccggctaca tcgatggcgg agccagccag gaagagttct acaagttcat caagcccatc
     4141 ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg
     4201 cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg
     4261 cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag
     4321 atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac
     4381 agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag
     4441 gaagtggtgg acaagggcgc cagcgcccag agcttcatcg agcggatgac caacttcgat
     4501 aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc
     4561 gtgtacaacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc
     4621 ctgagcggcg agcagaaaaa agccatcgtg gacctgctgt tcaagaccaa ccggaaagtg
     4681 accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtccttcga ctccgtggaa
     4741 atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa
     4801 attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc
     4861 gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat
     4921 gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg
     4981 ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc
     5041 ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac
     5101 gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc
     5161 ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag
     5221 acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc
     5281 gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag
     5341 agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac
     5401 cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg
     5461 cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac
     5521 catatcgtgc ctcagagctt tctgaaggac gactccatcg ataacaaagt gctgactcgg
     5581 agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg
     5641 aagaactact ggcgccagct gctgaatgcc aagctgatta cccagaggaa gttcgacaat
     5701 ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga
     5761 cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg
     5821 aacactaagt acgacgagaa cgacaaactg atccgggaag tgaaagtgat caccctgaag
     5881 tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac
     5941 aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa
     6001 aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg
     6061 aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac
     6121 agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag
     6181 cggcctctga tcgagacaaa cggcgaaaca ggcgagatcg tgtgggataa gggccgggac
     6241 tttgccaccg tgcggaaagt gctgtctatg ccccaagtga atatcgtgaa aaagaccgag
     6301 gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag cgacaagctg
     6361 atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag ccccaccgtg
     6421 gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt
     6481 gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc
     6541 gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct
     6601 aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa
     6661 ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc
     6721 agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg
     6781 gaacagcaca aacactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga
     6841 gtgatcctgg ccgacgctaa tctggacaag gtgctgagcg cctacaacaa gcacagagac
     6901 aagcctatca gagagcaggc cgagaatatc atcaacctgt ttaccctgac caatctggga
     6961 gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta caccagcacc
     7021 aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg
     7081 atcgacctgt ctcagctggg aggcgacgcc tatccctatg acgtgcccga ttatgccagc
     7141 ctgggcagcg gctcccccaa gaaaaaacgc aaggtggacg gcagcggaag caaaaggccg
     7201 gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagc tcgagtgaca ttggaagtgg
     7261 ataacggatc cgcgatcgcg gcgcgccacc tggtggccgg ccggtaccac gcgtgcgcgc
     7321 tgatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
     7381 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
     7441 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
     7501 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
     7561 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
     7621 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
     7681 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
     7741 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
     7801 aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
     7861 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     7921 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7981 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataacattg aaaaaggaag
     8041 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8101 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8161 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8221 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8281 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8341 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8401 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8461 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8521 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8581 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8641 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8701 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8761 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8821 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8881 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8941 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9001 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9061 atcaaaggat cttc
//
